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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
13/04/2021 |
Data da última atualização: |
13/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUSA, T. P. de; CHAIBUB, A. A.; CORTES, M. V. de C. B.; BATISTA, T. F. C.; BEZERRA, G. de A.; SILVA, G. B. da; FILIPPI, M. C. C. de. |
Afiliação: |
THATYANE PEREIRA DE SOUSA, UFG; AMANDA ABDALLAH CHAIBUB, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; TELMA FATIMA COELHO BATISTA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; GUSTAVO DE ANDRADE BEZERRA, UFG; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
Título: |
Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Archives of Microbiology, 2021. |
ISSN: |
0302-8933 |
DOI: |
https://doi.org/10.1007/s00203-021-02307-5 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. MenosThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M... Mostrar Tudo |
Palavras-Chave: |
Antagonism; Bioagent; Mycoparasitism. |
Thesagro: |
Antagonismo; Arroz; Brusone; Controle Biológico; Marcador Molecular; Oryza Sativa; Trichoderma. |
Thesaurus Nal: |
Biological control; Mycoparasites; Rice. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02690naa a2200373 a 4500 001 2131247 005 2021-04-13 008 2021 bl uuuu u00u1 u #d 022 $a0302-8933 024 7 $ahttps://doi.org/10.1007/s00203-021-02307-5$2DOI 100 1 $aSOUSA, T. P. de 245 $aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.$h[electronic resource] 260 $c2021 520 $aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. 650 $aBiological control 650 $aMycoparasites 650 $aRice 650 $aAntagonismo 650 $aArroz 650 $aBrusone 650 $aControle Biológico 650 $aMarcador Molecular 650 $aOryza Sativa 650 $aTrichoderma 653 $aAntagonism 653 $aBioagent 653 $aMycoparasitism 700 1 $aCHAIBUB, A. A. 700 1 $aCORTES, M. V. de C. B. 700 1 $aBATISTA, T. F. C. 700 1 $aBEZERRA, G. de A. 700 1 $aSILVA, G. B. da 700 1 $aFILIPPI, M. C. C. de 773 $tArchives of Microbiology, 2021.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
10/12/2013 |
Data da última atualização: |
24/02/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FRANCISCO, V. C.; ZENATTI, D. P.; PERSEGUINI, A. C.; FERREIRA, A. U. C.; PEREIRA, C. A. M.; NASSU, R. T. |
Afiliação: |
RENATA TIEKO NASSU, CPPSE. |
Título: |
Análise sensorial e qualidade da carne bovina proveniente de cruzamento entre raças adaptadas e não adaptadas. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: CONGRESSO DE INICIAÇÃO CIENTÍFICA UNICEP, 15., 2013, São Carlos, SP. Anais... São Carlos: UNICEP, 2013. |
Idioma: |
Português |
Palavras-Chave: |
Análise sensorial; Carne bovina; Raças adaptadas; Raças não adaptadas. |
Thesagro: |
Cruzamento; Qualidade. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/93745/1/PROCI-2013.00186.pdf
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Marc: |
LEADER 00764nam a2200229 a 4500 001 1973393 005 2016-02-24 008 2013 bl uuuu u00u1 u #d 100 1 $aFRANCISCO, V. C. 245 $aAnálise sensorial e qualidade da carne bovina proveniente de cruzamento entre raças adaptadas e não adaptadas. 260 $aIn: CONGRESSO DE INICIAÇÃO CIENTÍFICA UNICEP, 15., 2013, São Carlos, SP. Anais... São Carlos: UNICEP$c2013 650 $aCruzamento 650 $aQualidade 653 $aAnálise sensorial 653 $aCarne bovina 653 $aRaças adaptadas 653 $aRaças não adaptadas 700 1 $aZENATTI, D. P. 700 1 $aPERSEGUINI, A. C. 700 1 $aFERREIRA, A. U. C. 700 1 $aPEREIRA, C. A. M. 700 1 $aNASSU, R. T.
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