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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/03/2021 |
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Data da última atualização: |
11/11/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ZAMAN, M.; KLEINEIDAM, K.; BAKKEN, L.; BERENDT, J.; BRACKEN, C.; BUTTERBACH-BAHL, K.; CAI, Z.; CHANG, S. X.; CLOUGH, T.; DAWAR, K.; DING, W. X.; DÖRSCH, P.; MARTINS, M. dos R.; ECKHARDT, C.; FIEDLER, T.; FROSCH, T.; GOOPY, J.; GORRES, C. M.; GUPTA, A.; HENJES, S.; HOFMMAN, M. E. G.; HORN, M. A.; JAHANGIR, M. M. R.; JANSEN-WILLEMS, A.; LENHART, K.; HENG, L.; LEWICKA-SZCZEBAK, D.; LUCIC, G.; MERBOLD, L.; MOHN, J.; MOLSTAD, L.; MOSER, G.; MURPHY, P.; SANZ-COBENA, A.; SIMEK, M.; URQUIAGA, S.; WELL, R.; WRAGE-MÖNNIG, N.; ZAMAN, S.; SHANG, J.; MÜLLER, C. |
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Título: |
Isotopic techniques to Measure N2O, N2 and their sources. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: ZAMAN, M.; HENG, L.; Müller, C. (Ed.). Measuring emission of agricultural greenhouse gases and developing mitigation options using nuclear and related techniques: applications of nuclear techniques for GHGs. London: Springer, 2021. Chapter 7. |
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Páginas: |
p. 213-301 |
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ISBN: |
978-3-030-55396-8 |
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DOI: |
https://doi.org/10.1007/978-3-030-55396-8_7 |
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Idioma: |
Inglês |
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Conteúdo: |
GHG emissions are usually the result of several simultaneous processes. Furthermore, some gases such as N2 are very difficult to quantify and require special techniques. Therefore, in this chapter, the focus is on stable isotope methods. Both natural abundance techniques and enrichment techniques are used. Especially in the last decade, a number of methodological advances have been made. Thus, this chapter provides an overview and description of a number of current state-of-theart techniques, especially techniques using the stable isotope 15N. Basic principles and recent advances of the 15N gas flux method are presented to quantify N2 fluxes, but also the latest isotopologue and isotopomer methods to identify pathways for N2O production. The second part of the chapter is devoted to 15N tracing techniques, the theoretical background and recent methodological advances. A range of different methods is presented from analytical to numerical tools to identify and quantify pathway-specific N2O emissions. While this chapter is chiefly concerned with gaseous N emissions, a lot of the techniques can also be applied to other gases such as methane (CH4), as outlined in Sect. 5.3. |
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Palavras-Chave: |
N tracer technique. |
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Thesaurus Nal: |
climate change; greenhouse gas emissions. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 03115naa a2200673 a 4500
001 2130524
005 2022-11-11
008 2021 bl uuuu u00u1 u #d
020 $a978-3-030-55396-8
024 7 $ahttps://doi.org/10.1007/978-3-030-55396-8_7$2DOI
100 1 $aZAMAN, M.
245 $aIsotopic techniques to Measure N2O, N2 and their sources.$h[electronic resource]
260 $c2021
300 $ap. 213-301
520 $aGHG emissions are usually the result of several simultaneous processes. Furthermore, some gases such as N2 are very difficult to quantify and require special techniques. Therefore, in this chapter, the focus is on stable isotope methods. Both natural abundance techniques and enrichment techniques are used. Especially in the last decade, a number of methodological advances have been made. Thus, this chapter provides an overview and description of a number of current state-of-theart techniques, especially techniques using the stable isotope 15N. Basic principles and recent advances of the 15N gas flux method are presented to quantify N2 fluxes, but also the latest isotopologue and isotopomer methods to identify pathways for N2O production. The second part of the chapter is devoted to 15N tracing techniques, the theoretical background and recent methodological advances. A range of different methods is presented from analytical to numerical tools to identify and quantify pathway-specific N2O emissions. While this chapter is chiefly concerned with gaseous N emissions, a lot of the techniques can also be applied to other gases such as methane (CH4), as outlined in Sect. 5.3.
650 $aclimate change
650 $agreenhouse gas emissions
653 $aN tracer technique
700 1 $aKLEINEIDAM, K.
700 1 $aBAKKEN, L.
700 1 $aBERENDT, J.
700 1 $aBRACKEN, C.
700 1 $aBUTTERBACH-BAHL, K.
700 1 $aCAI, Z.
700 1 $aCHANG, S. X.
700 1 $aCLOUGH, T.
700 1 $aDAWAR, K.
700 1 $aDING, W. X.
700 1 $aDÖRSCH, P.
700 1 $aMARTINS, M. dos R.
700 1 $aECKHARDT, C.
700 1 $aFIEDLER, T.
700 1 $aFROSCH, T.
700 1 $aGOOPY, J.
700 1 $aGORRES, C. M.
700 1 $aGUPTA, A.
700 1 $aHENJES, S.
700 1 $aHOFMMAN, M. E. G.
700 1 $aHORN, M. A.
700 1 $aJAHANGIR, M. M. R.
700 1 $aJANSEN-WILLEMS, A.
700 1 $aLENHART, K.
700 1 $aHENG, L.
700 1 $aLEWICKA-SZCZEBAK, D.
700 1 $aLUCIC, G.
700 1 $aMERBOLD, L.
700 1 $aMOHN, J.
700 1 $aMOLSTAD, L.
700 1 $aMOSER, G.
700 1 $aMURPHY, P.
700 1 $aSANZ-COBENA, A.
700 1 $aSIMEK, M.
700 1 $aURQUIAGA, S.
700 1 $aWELL, R.
700 1 $aWRAGE-MÖNNIG, N.
700 1 $aZAMAN, S.
700 1 $aSHANG, J.
700 1 $aMÜLLER, C.
773 $tIn: ZAMAN, M.; HENG, L.; Müller, C. (Ed.). Measuring emission of agricultural greenhouse gases and developing mitigation options using nuclear and related techniques: applications of nuclear techniques for GHGs. London: Springer, 2021. Chapter 7.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
08/12/2004 |
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Data da última atualização: |
03/09/2018 |
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Autoria: |
COLLARES, E. A. S.; CHOER, E.; PEREIRA, A. da S. |
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Afiliação: |
Eliana Antonia Silveira Collares, Universidade Federal de Pelotas - UFPel/Colegiado de Pós-graduação em Agronomia; Eva Choer, CPACT; ARIONE DA SILVA PEREIRA, CPACT. |
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Título: |
Characterization of potato genotypes using molecular markers. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 9, p. 871-878, set. 2004. |
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Idioma: |
Inglês
Português |
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Notas: |
Título em português: Caracterização de genótipos de batata por marcadores moleculares. |
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Conteúdo: |
The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes. |
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Palavras-Chave: |
isoenzimas; RAPD; similaridade genética. |
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Thesagro: |
Batata; Eletroforese; Genótipo; Isoenzima; Marcador Molecular; Solanum Tuberosum. |
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Thesaurus NAL: |
electrophoresis; genetic similarity; isozymes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/29018/1/39n09a06.pdf
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Marc: |
LEADER 01781naa a2200301 a 4500
001 1112526
005 2018-09-03
008 2004 bl uuuu u00u1 u #d
100 1 $aCOLLARES, E. A. S.
245 $aCharacterization of potato genotypes using molecular markers.
260 $c2004
500 $aTítulo em português: Caracterização de genótipos de batata por marcadores moleculares.
520 $aThe objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.
650 $aelectrophoresis
650 $agenetic similarity
650 $aisozymes
650 $aBatata
650 $aEletroforese
650 $aGenótipo
650 $aIsoenzima
650 $aMarcador Molecular
650 $aSolanum Tuberosum
653 $aisoenzimas
653 $aRAPD
653 $asimilaridade genética
700 1 $aCHOER, E.
700 1 $aPEREIRA, A. da S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 39, n. 9, p. 871-878, set. 2004.
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