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| Registros recuperados : 92 | |
| 45. |  | LEGAT, A. P.; LEGAT, J. F. A.; PEREIRA, A. M. L.; DINIZ, F. M.; FOGACA, F. H. dos S. Conservation, use and management of marine resources and ecosystems. In: FOGAÇA, F. H. dos S.; FURTADO, A. A. L.; SILVA, C. A. da; TAVARES-DIAS; M.; ROUTLEDGE, E. A. B. (ed.). Life below water: contributions of Embrapa. Brasília, DF: Embrapa, 2020. cap. 3, p. 31-45. (Sustainable development goals, 14).| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Caprinos e Ovinos. |
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| 47. | | LEGAT, A. P.; LEGAT, J. F. A.; PEREIRA, A. M. L.; DINIZ, F. M.; FOGACA, F. H. dos S. Conservação, uso e gestão de recursos e ecossistemas marinhos. In: FOGACA, F. H. dos S.; FURTADO, A. A. L.; SILVA, C. A. da; TAVARES-DIAS, M.; ROUTLEDGE, E. A. B. (Ed.). Vida na água: contribuições da Embrapa. Brasília, DF: Embrapa, 2018. Cap. 3. E-book: il. color. (Objetivos de Desenvolvimento Sustentável, 14).| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Caprinos e Ovinos; Embrapa Meio-Norte. |
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| 53. | | SILVA, R. de S.; MENDES, W.; MAI, M. G.; FOGACA, F. H. dos S.; PEREIRA, A. M. L.; MAGALHAES, J. A.; COSTA, N. de L. Caracterização do processamento artesanal da carne de caranguejo-uçá Ucides cordatus e perfil socioeconômico dos quebradores. PUBVET, v. 11, n. 6, p. 566-574, Jun. 2017.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Roraima. |
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| 55. | | LEGAT, J. F. A.; LEGAT, A. P.; CASTRO, P. F. de; PEREIRA, A. M. L.; GÓES, J. M. de; GÓES, L. C. F. Current fishery status of Ucides cordatus (Linnaeus, 1763) (Brachyura, Ocypodidae) in the Parnaíba Delta Region, Brazil. Nauplius, v. 13, n. 1, p. 65-70, 2005.| Biblioteca(s): Embrapa Meio Norte / UEP-Parnaíba. |
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| 56. | | LEGAT, J. F. A.; LEGAT, A. P.; CASTRO, P. F.; PEREIRA, A. M. L.; GOES, J. M.; FERNANDES-GOES, L. C. Current status of ucides cordatus (linnaeus, 1763) (crustácea, ocypodidae) fishery in the Parnaíba delta region, Brazil. In: BRAZILIAN CRUSTACEAN CONGRESS & THE CRUSTACEAM SOCIETY MEETING, 3. 2004, Florianópolis, SC. Program and Abstracts. Florianópolis: SBC, 2004. p. 123.| Biblioteca(s): Embrapa Meio-Norte. |
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| 57. | | SANTOS, R. V. dos; FOGACA, F. H. dos S.; PEREIRA, A. M. L.; MIZAEL, F. H.; RODRIGUES, L. A.; MAGALHAES, J. A. Depuration of Anomalocardia brasiliana to increase the value of shellfish collected on the Piaui Coast, Brazil. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 18., 2016. Dublin. Greening the global food supply Chain: through innovation in food science and technology: Book of Proceedings. Dublin: IFSTI, 2016. IUFOST 2016.| Biblioteca(s): Embrapa Meio-Norte. |
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| 58. | | SANTANA, W. M.; SOUSA, F. J. A.; ANJOS, V. M.; SOUSA, J. M. S.; CASTRO, P. F.; PEREIRA, A. M. L. Desempenho produtivo do camarão Litopenaeus vannamei cultivado em águas interiores sob diferentes estratégias alimentares. In: CONGRESSO BRASILEIRO DE OCEANOGRAFIA, 3.; CONGRESSO IBERO-AMERICANO DE OCEANOGRAFIA, 1., 2008, Fortaleza. Anais... Fortaleza: Associação Brasileira de Oceanografia, 2008. 3 p.| Biblioteca(s): Embrapa Meio-Norte. |
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| 59. | | OLIVEIRA, V. Q. de; PIMENTEL, O. A. L. F.; OLIVEIRA, C. R. do R.; SANTOS, E. P. dos; PEREIRA, A. M. L.; GALVEZ, A. O.; BRITO, L. O. Effect of ionic adjustment frequency in low-salinity water on zootechnical performance, water quality and mineral composition of Litopenaeus vannamei in a synbiotic nursery system. Aquaculture, v. 561, 738632, 2022.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Tabuleiros Costeiros. |
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| 60. | | BORSA, M.; SEIBERT, C. H.; ROSA, R. D.; STOCO, P. H.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. Detection of infectious myonecrosis virus in penaeid shrimps using immunoassays: usefulness of monoclonal antibodies directed to the viral major capsid protein. Archives of Virology, New York, v. 156, n. 1, p. 9-16, jan. 2011.| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 92 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
28/12/2010 |
|
Data da última atualização: |
20/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SEIBERT, C. H.; BORSA, M.; ROSA, R. D.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. |
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Afiliação: |
CAROLINE H. SEIBERT, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MARIANA BORSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; RAFAEL D. ROSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; EDUARDO CARGNIN-FERREIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ALITIENE MOURA LEMOS PEREIRA, CPAMN; EDMUNDO C. GRISARD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; CARLOS R. ZANETTI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; AGUINALDO R. PINTO, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 169, n. 1, p. 169-175, 2010. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Infectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps. |
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Palavras-Chave: |
Proteína recombinante; Vírus da mionecrose infecciosa. |
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Thesagro: |
Anticorpo Monoclonal; Camarão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24511/1/JOURNAL.pdf
|
|
Marc: |
LEADER 02064naa a2200253 a 4500
001 1870999
005 2022-06-20
008 2010 bl uuuu u00u1 u #d
100 1 $aSEIBERT, C. H.
245 $aDetection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies.
260 $c2010
520 $aInfectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps.
650 $aAnticorpo Monoclonal
650 $aCamarão
653 $aProteína recombinante
653 $aVírus da mionecrose infecciosa
700 1 $aBORSA, M.
700 1 $aROSA, R. D.
700 1 $aCARGNIN-FERREIRA, E.
700 1 $aPEREIRA, A. M. L.
700 1 $aGRISARD, E. C.
700 1 $aZANETTI, C. R.
700 1 $aPINTO, A. R.
773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 169, n. 1, p. 169-175, 2010.
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