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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
07/12/2015 |
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Data da última atualização: |
11/02/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BARRETO, A. G.; NOGUEIRA, R. I.; MATTOS, L. da S. de; GODOY, R. C. B. de; FREITAS, S. P. |
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Afiliação: |
Angela Gava Barreto, CEFET/RJ; REGINA ISABEL NOGUEIRA, CTAA; LUZIMAR DA SILVA DE M DO NASCIMENTO, CTAA; ROSSANA CATIE BUENO DE GODOY, CNPF; Suely Pereira Freitas, UFRJ. |
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Título: |
Effect of drying temperature on the nutritional quality of the pinhão (araucária angustifolia) flour. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: EUROPEAN DRYING CONFERENCE, 5.; 2015, Budapest. Eurodrying'2015. Budapest: [s. n.], 2015. |
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Páginas: |
8 p. |
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Idioma: |
Inglês |
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Notas: |
Autoria: MATTOS, L. da S. de [i.e. NASCIMENTO, L. da S. de M.]. |
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Conteúdo: |
At present, there is quite limited market for pinhão products due to their low level of industrialization. This study aimed to evaluate the drying process of pinhão at 40 oC, 50 oC and 60 °C for essential amino acids preservation in the final product. Effective diffusivity (Deff) was 15.6x10-10m2.s-1at 60 oC and the correspondent activation energy was 31.13 kJ. mol-1. Compared to raw pinhão, it was observed greater retention of the amino acid content for the pinhão flour obtained at 50 oC. |
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Palavras-Chave: |
Drying kinetics. |
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Thesaurus Nal: |
activation energy; diffusivity; essential amino acids. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134962/1/Full-paper-Eurodrying-Angela-Gava-final.pdf
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Marc: |
LEADER 01288nam a2200229 a 4500
001 2030758
005 2016-02-11
008 2015 bl uuuu u00u1 u #d
100 1 $aBARRETO, A. G.
245 $aEffect of drying temperature on the nutritional quality of the pinhão (araucária angustifolia) flour.$h[electronic resource]
260 $aIn: EUROPEAN DRYING CONFERENCE, 5.; 2015, Budapest. Eurodrying'2015. Budapest: [s. n.]$c2015
300 $a8 p.
500 $aAutoria: MATTOS, L. da S. de [i.e. NASCIMENTO, L. da S. de M.].
520 $aAt present, there is quite limited market for pinhão products due to their low level of industrialization. This study aimed to evaluate the drying process of pinhão at 40 oC, 50 oC and 60 °C for essential amino acids preservation in the final product. Effective diffusivity (Deff) was 15.6x10-10m2.s-1at 60 oC and the correspondent activation energy was 31.13 kJ. mol-1. Compared to raw pinhão, it was observed greater retention of the amino acid content for the pinhão flour obtained at 50 oC.
650 $aactivation energy
650 $adiffusivity
650 $aessential amino acids
653 $aDrying kinetics
700 1 $aNOGUEIRA, R. I.
700 1 $aMATTOS, L. da S. de
700 1 $aGODOY, R. C. B. de
700 1 $aFREITAS, S. P.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F. |
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Afiliação: |
MAGDA L. T. SANTOS, UFERSA; ALANA A. BORGES, UFERSA; LUIZA B. QUEIROZ NETA, UFERSA; MARIA V. O. SANTOS, UFERSA; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA; ALEXANDRA F. PEREIRA, UFERSA. |
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Título: |
In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016. |
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Idioma: |
Português |
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Conteúdo: |
The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries. MenosThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve th... Mostrar Tudo |
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Palavras-Chave: |
Mitotic factor; Somatic tissue. |
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Thesagro: |
Tayassu Tajacu. |
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Thesaurus NAL: |
Culture media; Protein sources; Somatic cells; Wild animals. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155804/1/In-vitro-culutre-of-somatic.pdf
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Marc: |
LEADER 02511naa a2200277 a 4500
001 2064367
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. L. T.
245 $aIn vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.$h[electronic resource]
260 $c2016
520 $aThe maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
650 $aCulture media
650 $aProtein sources
650 $aSomatic cells
650 $aWild animals
650 $aTayassu Tajacu
653 $aMitotic factor
653 $aSomatic tissue
700 1 $aBORGES, A. A.
700 1 $aQUEIROZ NETA, L. B.
700 1 $aSANTOS, M. V. O.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
700 1 $aPEREIRA, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 12, p. 1194-1202, dez. 2016.
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