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| Registros recuperados : 3 | |
| 1. |  | JIMENEZ, C. R.; PENITENTE-FILHO, J. M.; TORRES, C. A. A.; MADEIROS, A. M.; SILVA, L. S. Vitrification of bovine preantral follicles with dimenthylsulfoxide and sucrose plus a-tocpherol. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 3, p. 209-215, mar. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | RODRIGUES, J. N. D.; GUIMARÃES, J. D.; FONSECA, J. F. da; PENITENTE-FILHO, J. M.; RANGEL, P. S. C.; RAMÍREZ LÓPEZ, C. J.; FREITAS, R. S.; CASTILHO, E. F. de; RODRIGUES, L. F. de S.; OLIVEIRA, M. E. F.; GARCIA, A. R. Climatic seasons and time of the day influence thermoregulation and testicular hemodynamics in Santa Inês rams raised under humid tropical conditions. Journal of Thermal Biology, v. 114, article 103546, May, 2023.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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| 3. | | DIAS, J. H.; VERGANI; GONÇALVES, J. D.; OLIVEIRA, T. A.; PENITENTE-FILHO, J. M.; PEREIRA, V. S. do A.; ESTEVES, S. N.; GARCIA, A. R.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Different doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes. Small Ruminant Research, v. 220, n. 103, mar. 2023, 106914.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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| Registros recuperados : 3 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
08/04/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
JIMENEZ, C. R.; PENITENTE-FILHO, J. M.; TORRES, C. A. A.; MADEIROS, A. M.; SILVA, L. S. |
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Afiliação: |
CAROLINA R. JIMENEZ, USP; JURANDY M. PENITENTE-FILHO, UFV; CIRO A. A. TORRES, UFV; AMANDA M. MEDEIROS, UFV; LEANDRO S. SILVA, UFV. |
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Título: |
Vitrification of bovine preantral follicles with dimenthylsulfoxide and sucrose plus a-tocpherol. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 3, p. 209-215, mar. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the vitrification of bovine preantral follicles with dimethylsulfoxide (D) and sucrose (S) plus α-tocopherol 5mmol/L (T5) or 10mmol/L (T10) and, evaluate the thawed with minimal essential medium (m) with or without sucrose (s). Ovaries of cows were collected from slaughterhouse for the experiment I (n=66) and II (n=51). In the laboratory ovarian fragments were randomly assigned either to fresh control and 8 vitrification treatments (Controle and Dm; Dms, DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Ovarian fragments were placed in vitrification solution (5 min) and immersed in liquid nitrogen (-196°C), after a week, the fragments were thawed and analyzed. In the experiments I, preantral follicles were morphologically observed for histological evaluation, (normal; degenerated and developing of stage). In the experiment II, preantral follicles were mechanically isolated from ovarian tissue and examined with trypan blue, where dead and live corresponded to stained or non-stained. The treatments DSm, DSms and DST10m were effective in preserving the morphology in situ. However, the viability of isolated preantral follicles after vitrification remained high only in treatment DST10m. Thus, DST10m preserves survival rates and morphological integrity during vitrification of bovine preantral follicles. |
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Palavras-Chave: |
Células granulosas; Crioprotetor; Dimenthylsulfoxide; Dimetilsulfóxido; Oócito. |
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Thesagro: |
Antioxidante; Criopreservação. |
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Thesaurus NAL: |
antioxidants; Cryopreservation; Granulosa cells; Oocytes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142197/1/Vitrificantion-of-bovine.pdf
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Marc: |
LEADER 02241naa a2200301 a 4500
001 2042974
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aJIMENEZ, C. R.
245 $aVitrification of bovine preantral follicles with dimenthylsulfoxide and sucrose plus a-tocpherol.
260 $c2016
520 $aThe objective of this study was to evaluate the vitrification of bovine preantral follicles with dimethylsulfoxide (D) and sucrose (S) plus α-tocopherol 5mmol/L (T5) or 10mmol/L (T10) and, evaluate the thawed with minimal essential medium (m) with or without sucrose (s). Ovaries of cows were collected from slaughterhouse for the experiment I (n=66) and II (n=51). In the laboratory ovarian fragments were randomly assigned either to fresh control and 8 vitrification treatments (Controle and Dm; Dms, DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Ovarian fragments were placed in vitrification solution (5 min) and immersed in liquid nitrogen (-196°C), after a week, the fragments were thawed and analyzed. In the experiments I, preantral follicles were morphologically observed for histological evaluation, (normal; degenerated and developing of stage). In the experiment II, preantral follicles were mechanically isolated from ovarian tissue and examined with trypan blue, where dead and live corresponded to stained or non-stained. The treatments DSm, DSms and DST10m were effective in preserving the morphology in situ. However, the viability of isolated preantral follicles after vitrification remained high only in treatment DST10m. Thus, DST10m preserves survival rates and morphological integrity during vitrification of bovine preantral follicles.
650 $aantioxidants
650 $aCryopreservation
650 $aGranulosa cells
650 $aOocytes
650 $aAntioxidante
650 $aCriopreservação
653 $aCélulas granulosas
653 $aCrioprotetor
653 $aDimenthylsulfoxide
653 $aDimetilsulfóxido
653 $aOócito
700 1 $aPENITENTE-FILHO, J. M.
700 1 $aTORRES, C. A. A.
700 1 $aMADEIROS, A. M.
700 1 $aSILVA, L. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 3, p. 209-215, mar. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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