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| Registros recuperados : 58 | |
| 41. |  | LIMA, C. C. V. de; AYRES, M. C. C; PINHEIRO, R. R.; COSTA, J. N.; ANDRIOLI, A.; SOUZA, T. S. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; DAMASCENO, E. M.; SILVA, J. B. R. da. Lentivírus caprino no sêmen de ovinos. Biológico, São Paulo, v. 77, p. 119, 2015. Suplemento 2, ref. 238. Edição dos Resumos XI Congresso Brasileiro de Buiatria, XVII Congresso Latinoamericano de Buiatria, jul. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 42. | | PEIXOTO, R. M.; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; SOUSA, M. M. de; AZEVEDO, D. A. A. de; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Mycoplasma agalactiae em rebanhos leiteiros no estado do Ceará em associação com o vírus da artrite encefalite caprina. Acta Scientiae Veterinariae, v. 46, Pub. 1533, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 43. | | AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. Small Ruminant Research, v. 177, p. 160-166, 2019.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
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| 44. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; PINHEIRO, A. A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul./set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 45. | | SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAÚJO, J. F.; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; ANDRIOLI, A.; BEZERRA, S. T. da C. S.; TEIXEIRA, M. F. da S. Sodium dodecyl sulfate as a viral inactivator and future perspectives in the control of small ruminant lentiviruses. Arquivos do Instituto Biológico, v. 86, p. 1-9, e0752018, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 46. | | ARAÚJO, J. F.; PINHEIRO, R. R.; ANDRIOLI, A. A.; ALVES, F. S. F.; FACCIOLI-MARTINS, P. Y.; ELOY, A. M. X.; SANTOS, V. W. S. dos; PEIXOTO, R. M.; LIMA, A. M. C. Soroprevalência e fatores de risco da infecção por Chlamydophila abortus em caprinos do estado do Rio Grande do Norte, Brasil. Acta Scientiae Veterinariae, v. 46, Pub. 1592, 2018. [ALICE ANDRIOLI PINHEIRO].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 47. | | PEIXOTO, R. M.; MAGALHÃES, J. L. L.; PEREIRA, T. M. A.; VASCONCELOS FILHO, P. de T.; LANDIM, A. V.; LIMA, F. R. G.; LEITE, E. R.; VASCONCELOS, A. M. de. Custo da alimentação de cabritos leiteiros submetidos a diferentes períodos de aleitamento, criados na região de Sobral-CE. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 22., 2012, Cuiabá. Anais... Cuiabá: Universidade Federal de Mato Grosso, 2012. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 48. | | AZEVEDO, D. A. A. de; PINHEIRO, R. R.; SANTOS, V. W. S. dos; DAMASCENO, E. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Comparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados. Acta Scientiae Veterinariae, v. 47, Pub. 1668, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 49. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; SOUSA, M. M. de; AMARAL, G. R. Prevalência sorológica de Mycoplasma agalactiae em rebanhos de caprinos leiteiros na mesorregião metropolitana de Fortaleza - CE. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 12., 2017, Juazeiro, BA. Construindo pontes entre o ensino, a pesquisa e a extensão: anais. Petrolina: Univasf: Embrapa Semiárido: Instituto Federal de Educação, Ciência e Tecnologia, Sertão de Pernambuco, 2017. 1 CD-ROM. p. 1284-1286.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 50. | | MARTINS, R. de J.; VESCHI, J. L. A.; CARMO, F. B.; AZEVEDO, V.; SEYFFERT, N; MIYOSHI, A.; MEYER, R.; PORTELA, R.; PEIXOTO, R. M.; COSTA, M. M. da; ZAFALON, L. F.; GOUVEIA, A. M. G. Avaliação da presença de anticorpos anti-Corynebacterium pseudotuberculosis em caprinos leiteiros do Território do Sisal, BA. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2010. p. 25-31. (Embrapa Semiárido. Documentos, 228.).| Biblioteca(s): Embrapa Semiárido. |
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| 51. | | PEIXOTO, R. M.; ANDRIOLI, A.; SANTOS, D. O.; PINHEIRO, R. R.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SILVA, D. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Avaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado. Acta Scientiae Veterinariae, v. 45, n. 1487, p. 1-8, 2017. [ALICE ANDRIOLI PINHEIRO].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 52. | | ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 53. | | ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. Vertical transmissibility of small ruminant lentivirus. PLoSONE, v. 15, n. 11, e0239916, Nov. 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 54. | | SANTOS, K. F. dos; ELOY, A. M. X.; MATOS, M. N. C.; PEIXOTO, R. M.; ARAGÃO, P. de T. T. D. de; PINHEIRO, R. R.; CUNHA, R. M. S. da. Use of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma. Small Ruminant Research, v. 181, p. 39-44, Dec. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 55. | | SOUZA, S. C. R.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; ANDRIOLI, A.; LIMA, A. M. C.; MENDES, B. K. M.; MAGALHÃES, N. M. de A.; AMARAL, G. P.; TEIXEIRA, M. F. da S. In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk. Brazilian Journal of Microbiology, v. 54, n. 3, Sept. 2023. On-line first.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 56. | | SOUSA, A. L. M.; PINHEIRO, R. R.; ARAÚJO, J. F.; SANTOS, V. W. S.; AZEVEDO, D. A. A.; PEIXOTO, R. M.; SOUZA, V. de; ANDRIOLI, A.; DAMASCENO, E. M.; DANTAS, T. V. M.; TEIXEIRA, M. F. S. In vitro and in vivo evaluation of sodium dodecyl sulfate (SDS) as an inactivator of caprine lentivirus (CLV) in colostrum and milk. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 70, n. 5, p. 1459-1467, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 57. | | LIMA, C. C. V.; AYRES, M. C. C.; PINHEIRO, R. R.; COSTA, J. N.; ANDRIOLI, A.; SOUZA, T. S.; AZEVEDO, D. A. A.; SANTOS, V. W. S.; ARAÚJO, J. F.; SOUSA, A. L. M.; PEIXOTO, R. M.; DAMASCENO, E. M.; COSTA NETO, A. O. Caprine lentivirus in sheep milk and semen. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 69, n. 2, mar./apr. 2017. [ALICE ANDRIOLI PINHEIRO].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 58. | | SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAUJO, J. F.; PEIXOTO, R. M.; AZEVEDO, D. A. A. de; LIMA, A. M. C.; CANUTO, K. M.; RIBEIRO, P. R. V.; SOUZA, A. S. de Q.; SOUZA, S. C. R.; AMORIM, S. L. de; AMARAL, G. P.; SOUZA, V. de; MORAIS, S. M. de; ANDRIOLI, A.; TEIXEIRA, M. F. da S. In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk. Scientific Reports, v. 13, article 4677, Mar. 2023.| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 58 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO, State University of Ceará - Fortaleza, Ceará, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO, Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO, State University of Ceará - Fortaleza, Ceará, Brazil; ANA MILENA CESAR LIMA, Federal University of Piauí - Teresina, Piauí, Brazil; JULIANA ARAÚJO NOBRE, State University of Ceará - Fortaleza, Ceará, Brazil; GABRIEL PAULA AMARAL, State University of Acaraú Valley, Sobral, Ceará, Brazil; IANE SOUSA BRANDÃO, State University of Acaraú Valley, Sobral, Ceará, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, State University of Ceará - Fortaleza, Ceará, Brazil. |
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Título: |
Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021. |
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DOI: |
https://doi.org/10.1016/j.cimid.2021.101693 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission. MenosAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally i... Mostrar Tudo |
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Palavras-Chave: |
Amniotic sac; Intrauterine transmission; Retrovirus; SRLVs; Vertical disease transmission. |
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Thesaurus NAL: |
Body fluids; Disease diagnosis; Goat diseases; Goats; Risk factors. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.cimid.2021.101693$2DOI
100 1 $aARAÚJO, J. F.
245 $aDetection and isolation of small ruminant lentivirus in the amniotic fluid of goats.$h[electronic resource]
260 $c2021
520 $aAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.
650 $aBody fluids
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aRisk factors
653 $aAmniotic sac
653 $aIntrauterine transmission
653 $aRetrovirus
653 $aSRLVs
653 $aVertical disease transmission
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aPEIXOTO, R. M.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aLIMA, A. M. C.
700 1 $aNOBRE, J. A.
700 1 $aAMARAL, G. P.
700 1 $aBRANDÃO, I. S.
700 1 $aTEIXEIRA, M. F. da S.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 78, 101693, Oct. 2021.
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