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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
11/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, B. A. de O.; FERREIRA, A. L.; ACUNHA, R. M. G.; ALMEIDA, R. G. dos S.; SANTOS, J. G. dos; FERNANDES, C. E.; CHAVES, F. C. M.; CHAGAS, E. C.; CARDOSO, C. A. L.; CAMPOS, C. M. de; COSTA, D. C. |
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Afiliação: |
BRENDA APARECIDA DE OLIVEIRA SILVA, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; ANDRE LIMA FERREIRA, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; RUBIA MARA GOMES ACUNHA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; RÔMULLO GUILHERME DOS SANTOS ALMEIDA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; JUSSARA GARRIDO DOS SANTOS, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; CARLOS EURICO FERNANDES, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; FRANCISCO CELIO MAIA CHAVES, CPAA; EDSANDRA CAMPOS CHAGAS, CPAA; CLAUDIA ANDREA LIMA CARDOSO, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; CRISTIANE MELDAU DE CAMPOS, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; DELIANE CRISTINA COSTA, UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL. |
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Título: |
Anesthetic efficiency of essential oil of Zingiber officinale for Astyanax lacustris: induction time, recovery time, ventilatory frequency, and gill histopathology. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Aquaculture International, 2023. |
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DOI: |
https://doi.org/10.1007/s10499-023-01344-9 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
This study tested the use of the essential oil of Zingiber ofcinale (EOZO) as an anesthetic for Astyanax lacustris and its possible histopathological efects on the gills. |
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Palavras-Chave: |
Gill damage; Ginger essential oil; Lambari-do-rabo-amarelo; Phytoconstituent; Zingiber ofcinale. |
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Thesagro: |
Óleo Essencial. |
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Thesaurus Nal: |
Anesthesia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01272naa a2200349 a 4500
001 2159963
005 2024-01-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10499-023-01344-9$2DOI
100 1 $aSILVA, B. A. de O.
245 $aAnesthetic efficiency of essential oil of Zingiber officinale for Astyanax lacustris$binduction time, recovery time, ventilatory frequency, and gill histopathology.$h[electronic resource]
260 $c2023
500 $aOnline first.
520 $aThis study tested the use of the essential oil of Zingiber ofcinale (EOZO) as an anesthetic for Astyanax lacustris and its possible histopathological efects on the gills.
650 $aAnesthesia
650 $aÓleo Essencial
653 $aGill damage
653 $aGinger essential oil
653 $aLambari-do-rabo-amarelo
653 $aPhytoconstituent
653 $aZingiber ofcinale
700 1 $aFERREIRA, A. L.
700 1 $aACUNHA, R. M. G.
700 1 $aALMEIDA, R. G. dos S.
700 1 $aSANTOS, J. G. dos
700 1 $aFERNANDES, C. E.
700 1 $aCHAVES, F. C. M.
700 1 $aCHAGAS, E. C.
700 1 $aCARDOSO, C. A. L.
700 1 $aCAMPOS, C. M. de
700 1 $aCOSTA, D. C.
773 $tAquaculture International, 2023.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
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Título: |
Vertical transmissibility of small ruminant lentivirus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
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Palavras-Chave: |
SRLV. |
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Thesaurus NAL: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
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Marc: |
LEADER 02915naa a2200325 a 4500
001 2127175
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI
100 1 $aARAÚJO, J. F.
245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource]
260 $c2020
520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
650 $aGenetic techniques and protocols
650 $aGoat diseases
650 $aGoats
650 $aLentivirus
650 $aSheep diseases
653 $aSRLV
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aSIDER, L. H.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aDAMASCENO, E. M.
700 1 $aSOUZA, S. C. R.
700 1 $aTEIXEIRA, M. F. da S.
773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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