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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/01/2018 |
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Data da última atualização: |
16/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, P. C. B.; BRONDANI, C.; MENEZES, I. P. P.; VALDISSER, P. A. M. R.; BORBA, T. C. O.; DEL PELOSO, M. J.; VIANELLO, R. P. |
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Afiliação: |
P. C. B. CARDOSO; CLAUDIO BRONDANI, CNPAF; I. P. P. MENEZES; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 1, p. 1964-1978, 2014. |
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DOI: |
10.4238/2014.March.24.1 |
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Idioma: |
Inglês |
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Conteúdo: |
Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders. MenosAnalysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not dif... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética. |
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Thesagro: |
DNA; Feijão; Marcador molecular; Phaseolus vulgaris. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171156/1/CNPAF-2014-gmr.pdf
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Marc: |
LEADER 02835naa a2200265 a 4500
001 2085520
005 2018-01-16
008 2014 bl uuuu u00u1 u #d
024 7 $a10.4238/2014.March.24.1$2DOI
100 1 $aCARDOSO, P. C. B.
245 $aDiscrimination of common bean cultivars using multiplexed microsatellite markers.$h[electronic resource]
260 $c2014
520 $aAnalysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders.
650 $aDNA
650 $aFeijão
650 $aMarcador molecular
650 $aPhaseolus vulgaris
653 $aDiversidade genética
700 1 $aBRONDANI, C.
700 1 $aMENEZES, I. P. P.
700 1 $aVALDISSER, P. A. M. R.
700 1 $aBORBA, T. C. O.
700 1 $aDEL PELOSO, M. J.
700 1 $aVIANELLO, R. P.
773 $tGenetics and Molecular Research$gv. 13, n. 1, p. 1964-1978, 2014.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 11. |  | PAZ, F. das C. A.; BRITO, P. F. A.; ABOMORAD, F. J. E.; CASCAIS, F. de A. A. Comportamento preliminar de clones de seringueira em Rio Branco-AC. In: SEMINÁRIO BRASILEIRO SOBRE RECOMENDAÇÕES DE CLONES DE SERINGUEIRA, 1., 1982, Brasília, DF. Anais... Brasília, DF: SUDHEVEA: EMBRAPA: EMBRATER, 1983. p. 183-185.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 13. | | CUENCA, M. A. G.; MOREIRA, M. A. B.; NUNES, M. U. C.; MATA, S. S. da; GUES, C. G. M.; BARRETO, M. de F. P.; LOPES, V. R. M.; PAZ, F. das C. A.; SILVA, J. R. da; TORRES, J. F. Perfil do consumidor e do consumo de produtos orgânicos no Rio Grande do Norte. Aracaju: Embrapa Tabuleiros Costeiros, 2007. 17 p. (Embrapa Tabuleiros Costeiros. Documentos, 125).| Tipo: Documentos | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 14. | | CUENCA, M. A. G.; MOREIRA, M. A. B.; NUNES, M. U. C.; MATA, S. S. da; GUES, C. G. M.; BARRETO, M. de F. P.; LOPES, V. R. M.; PAZ, F. das C. A.; SILVA, J. R. da; TORRES, J. F. Caracterização das bases de produção orgância no Rio Grande do Norte. Aracaju: Embrapa Tabuleiros Costeiros, 2007. 21 p. (Embrapa Tabuleiros Costeiros. Documentos, 124).| Tipo: Documentos | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 14 | |
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