| |
|
|
| Registros recuperados : 71 | |
| 22. |  | CATTANI, A. M.; SILVEIRA, C. P.; BUFFON, V.; PASQUALI, G.; REVERS, L. F. Compreendendo a dormência de macieira utilizando a integração de sinais externos e mecanismos genéticos. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 17., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 13., 2019, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 18 e 19 de julho de 2019. (Embrapa Uva e Vinho. Documentos, 115). Resumo 06.| Biblioteca(s): Embrapa Uva e Vinho. |
|    |
| 25. | | CATTANI, A. M.; SILVEIRA, C. P.; PASQUALI, G.; REVERS, L. F. Reguladores de resposta do tipo B envolvidos na via de sinalização de citocininas auxiliam na integração entre estímulo hormonal e resposta molecular durante o ciclo de dormência de macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 16.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 12., 2018, Bento Gonçalves. Anais... Bento Gonçalves, RS: Embrapa Uva e Vinho, 26 a 27 de setembro de 2018. p. 23.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 29. | | GRATTAPAGLIA, D.; NOVAES, E.; PAPPAS, G. J.; PASQUALI, G.; KIRST, M. Single feature polymorphism discovery and validation in Eucalyptus by pseudo-testcross inheritance and mapping. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, W172.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 30. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARASCHIN, F. S.; PASQUALI, G.; MÁRGIS-PINHEIRO, M. N.; REVERS, L. F. Avaliação da função de VvAGL11 na morfogênese da semente da videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 46. (Embrapa Uva e Vinho. Documentos, 99)| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 31. | | FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Perfil transcricional em gemas dormentes de duas cultivares de macieira com requerimento de frio contrastante. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 13. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 32. | | MIOTTO, Y. E.; FALAVIGNA, V. S.; PORTO, D. D.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Dormancy-related transcript accumulation of the dehydrin gene family in apple buds. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 1. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 34. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; MARASHIN, F. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.). Plant Science, v. 269, p. 126-135, 2018.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 35. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. 179 510-519| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 36. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2010. p. 206. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 37. | | MIOTTO, Y. E.; FALAVIGNA, V. da S.; PORTO, D. D.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Identificação, classificação e caracterização transcricional das desidrinas de macieira. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 620_623, 2012.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 38. | | GARIGHAN, J. de A.; PASQUALI, G.; REVERS, L. F.; PORTO, D. D.; SOUZA, D. A.; SANTOS, H. P. dos. Hormonal balance characterization of dormant buds in apple. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 683.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 39. | | FALAVIGNA, V. da S.; MIOTTO, Y. E.; PORTO, D. D.; ANZANELLO, R.; FIALHO, F. B.; SANTOS, H. P. dos; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Análise da expressão de genes codificadores de desidrinas durante a dormência de gemas em macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 20. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 40. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. Journal of Experimental Botany, v. 68, n. 7, p. 1493-1506, mar. 2017.| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| Registros recuperados : 71 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
28/08/2014 |
|
Data da última atualização: |
02/04/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
PERINI, P.; PASQUALI, G.; MARGIS-PINHEIRO, M.; OLIVEIRA, P. R. D. de; REVERS, L. F. |
|
Afiliação: |
Pâmela Perini; Giancarlo Pasquali; Márcia Margis-Pinheiro; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV. |
|
Título: |
Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.). |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Molecular Breeding, mar. 2014. |
|
Idioma: |
Inglês |
|
Notas: |
DOI 10.1007/s11032-014-0078-3 |
|
Conteúdo: |
Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple. MenosApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances u... Mostrar Tudo |
|
Palavras-Chave: |
Expressão genética; Gala; Genes de referencia; RT-qPCR. |
|
Thesagro: |
Genetica vegetal; Maçã. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107436/1/Perini2014-Reference-Genes-Apple.pdf
|
|
Marc: |
LEADER 02287naa a2200253 a 4500
001 1993623
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aPERINI, P.
245 $aReference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.).$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11032-014-0078-3
520 $aApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple.
650 $aGenetica vegetal
650 $aMaçã
653 $aExpressão genética
653 $aGala
653 $aGenes de referencia
653 $aRT-qPCR
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aOLIVEIRA, P. R. D. de
700 1 $aREVERS, L. F.
773 $tMolecular Breeding, mar. 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
