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| Registros recuperados : 13 | |
| 1. |  | MATOS, A.; TORREZAN, R.; DEL AGUILA, E. M.; OLIVEIRA, E. M. M.; PASCHOALIN, V. M. F. Evaluación de soya geneticamente modificada por Q-PCR en el procesamiento de leche de soya. In: CONGRESO IBEROAMERICANO DE INGENIERÍA DE ALIMENTOS, 9., 2014, Valencia. Libro de actas. Valencia: Universitat Politècnia de València, 2014. v. 2, p. 505-511.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | BARROS, N. E. F.; OLIVEIRA, E. M. M.; FREITAS-SILVA, O.; SILVA, J. T.; PASCHOALIN, V. M. F. Assessment of genetically modified soybean in Brazilian processed foods. In: WORLD CONGRESS OF FOOD SCIENCE & TECHNOLOGY, 13., 2006, Nates, France. Abstracts... Nates: [s. n.], 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 5. | | PINTO JUNIOR, W. R.; DEL AGUILA, E. M.; SILVA, J. M.; SILVA, J. T.; ROSENTAL, A.; PASCHOALIN, V. M. F. Determination of proteolytic activity of metalloproteinase from industrial strains isolated from the a dairy industry-plant. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2013.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 6. | | BARROS, N. E. F. de; OLIVEIRA, E. M. M.; SILVA, O. F.; SILVA, J. T.; PASCHOALIN, V. M. F. Qualitative and quantitative assessment of genetically modified soy in enteral nutrition formulas by polymerase chain reaction based methods. Revista de Nutrição, Campinas, v. 23, n. 1, p. 49-55, jan./fev. 2010.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 8. | | MATOS, A.; DEL AGUILA, E. M.; COSTA, S. D. O.; PENTEADO, A. L.; PENTEADO, M. S.; TORREZAN, R.; DELIZA, R.; OLIVEIRA, E. M. M.; PASCHOALIN, V. M. F. Quality assessment of soy milk: RR® event traceability, detection of Salmonella enteritidis and sensory analysis. In: CHEM RIO SYMPOSIUM, 14., 2014, Rio de Janeiro. Anais... Rio de Janeiro: UFRJ, Instituto de Química, 2014.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | NUNES, R. S. C.; PINHATI, F. R.; GOLINELLI, L. P.; REBOUÇAS, T. N. H.; PASCHOALIN, V. M. F.; SILVA, J. T. da. Polymorphic microsatellites of analysis in cultivars of taro. Horticultura Brasileira, Brasilia, DF, v. 30, n. 1, p. 106-111, jan./mar. 2012.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | GOLINELLI, L. P.; CARVALHO, A. C.; CASAES, R. S.; LOPES, C. S. C.; DELIZA, R.; PASCHOALIN, V. M. F.; SILVA, J. T. Sensory analysis and species-specific PCR detect bovine milk adulteration of frescal (fresh) goat cheese. Journal of Dairy Science, v. 97, n. 11, p. 6693-6699, Nov., 2014.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | PINTO JUNIOR, W. R.; PEREIRA, P. R.; SILVA, J. M.; DEL AGUILA, E. M.; SILVA, J. T.; PASCHOALIN, V. M. F.; ROSENTHAL, A. Uso de los tra amientos termico e no termico (alta presion hidrostatica) para la inactivación de enzimas bacterianas metaloproteases presentes en la degradación de leche. In: CONGRESO IBEROAMERICANO DE INGENIERÍA DE ALIMENTOS, 9., 2014, Valencia. Libro de actas. Valencia: Universitat Politècnia de València, 2014. v. 2, p. 497-504.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | SANTOS, A. M. dos; LIMA, I.; DEL AGUILA, E. M.; COSTA, S. D. de O.; PENTEADO, A. L.; STEPHAN, M. P.; TORREZAN, R.; DELIZA, R.; OLIVEIRA, E. M. M.; PASCHOALIN, V. M. F. Quality assessment of soy milk: RR® event traceability, detection of Salmonella Enteritidis and sensory analysis. In: CHEM RIO SYMPOSIUM, 14., 2014, Rio de Janeiro. Anais...Rio de Janeiro: Instituto de Química/UFRJ, 2014.| Biblioteca(s): Embrapa Solos. |
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| 13. | | CARVALHO, R. C. T.; VASCONCELLOS, S. E. G.; ISSA, M. de A.; SOARES FILHO, P. M.; MOTA, P. M. P. C.; ARAUJO, F. R.; CARVALHO, A. C. da S.; GOMES, H. M.; SUFFYS, P. N.; FIGUEIREDO, E. E. de S.; PASCHOALIN, V. M. F. Molecular typing of mycobacterium bovis from cattle reared in Midwest Brazil. PlOs ONE, v. 11, n. 9, p. 1-16, Sept. 2016.| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
19/01/2017 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CARVALHO, R. C. T.; VASCONCELLOS, S. E. G.; ISSA, M. de A.; SOARES FILHO, P. M.; MOTA, P. M. P. C.; ARAUJO, F. R.; CARVALHO, A. C. da S.; GOMES, H. M.; SUFFYS, P. N.; FIGUEIREDO, E. E. de S.; PASCHOALIN, V. M. F. |
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Afiliação: |
RICARDO CÉSAR TAVARES CARVALHO, UFRJ; SIDRA EZIDIO GONÇALVES VASCONCELLOS, FIOCRUZ; MARINA DE AZEVEDO ISSA, LANAGRO/MAPA; PAULO MARTINS SOARES FILHO, LANAGRO/MAPA; PEDRO MOACYR PINTO COELHO MOTA, LANAGRO/MAPA; FLABIO RIBEIRO ARAUJO, CNPGC; ANA CAROLINA DA SILVA CARVALHO, UFRJ/UFMT; HARRISON MAGDINIER GOMES, FIOCRUZ; PHILIP NOEL SUFFYS, FIOCRUZ/LANAGRO/MAPA; EDUARDO EUSTÁQUIO DE SOUZA FIGUEIREDO, UFMT; VÂNIA MARGARET FLOSI PASCHOALIN, UFRJ. |
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Título: |
Molecular typing of mycobacterium bovis from cattle reared in Midwest Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PlOs ONE, v. 11, n. 9, p. 1-16, Sept. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = > 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions,municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil. MenosMycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = > 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patter... Mostrar Tudo |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus NAL: |
Bovine tuberculosis; Brazil; Cattle; Molecular epidemiology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153526/1/Molecular-Typing-of-mycobacterium.pdf
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Marc: |
LEADER 02614naa a2200301 a 4500
001 2061179
005 2017-01-19
008 2016 bl uuuu u00u1 u #d
100 1 $aCARVALHO, R. C. T.
245 $aMolecular typing of mycobacterium bovis from cattle reared in Midwest Brazil.$h[electronic resource]
260 $c2016
520 $aMycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = > 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions,municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.
650 $aBovine tuberculosis
650 $aBrazil
650 $aCattle
650 $aMolecular epidemiology
650 $aMycobacterium Bovis
700 1 $aVASCONCELLOS, S. E. G.
700 1 $aISSA, M. de A.
700 1 $aSOARES FILHO, P. M.
700 1 $aMOTA, P. M. P. C.
700 1 $aARAUJO, F. R.
700 1 $aCARVALHO, A. C. da S.
700 1 $aGOMES, H. M.
700 1 $aSUFFYS, P. N.
700 1 $aFIGUEIREDO, E. E. de S.
700 1 $aPASCHOALIN, V. M. F.
773 $tPlOs ONE$gv. 11, n. 9, p. 1-16, Sept. 2016.
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Embrapa Gado de Corte (CNPGC) |
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