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| Registros recuperados : 99 | |
| 3. |  | NEVES, L. G.; PAPPAS JUNIOR, G. J.; PAQUALI, G.; KIRST, M.; GRATTAPAGLIA, D. Advancing to a high density gene-rich map based on single feature polymorphisms in tropical eucalyptus. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 17., 2009, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2009. W185| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | TESSELE, C.; REVERS, L. F.; ALENCAR, S. A.; PAPPAS JUNIOR, G. J.; DELATORRE, C. A. Construção de um mapa genético de macieira de alta densidade utilizando marcadores funcionais tipo SNP. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 4574-4577, 2012.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | SOARES, G. B.; TOGAWA, R. C.; MARTINS, N. F.; PAPPAS JUNIOR, G. J. Geração e análise de seqüências genômicas por meio de serviços Web pela ferramenta taverna. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 036. p. 76.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | SENA, J. S.; MAMANI, E. M.; PAPPAS JUNIOR, G. J.; GRATTAPAGLIA, D. Mapeamento e caracterização genética de novos microssatélites derivados de EST em Eucalyptus grandis x Eucalyptus urophylla. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. P. 179. Disponível também em: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2007. CDR0057.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | SENA, J. S.; PÁDUA, J. G.; PAPPAS JÚNIOR, G. J.; GRATTAPAGLIA, D. Mapeamento de microssatélites derivados de EST em cruzamentos intra e interespecíficos de Eucalyptus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 91.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | COSTA, M. B. W.; TREPTOW, W.; ARAÚJO, A. F. P.; PAPPAS JUNIOR, G. J. Predição de enterramentos atômicos de proteínas globulares utilizando-se estatística Bayesiana e linguagem Perl de bioinformática. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 028. p. 64| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | SENA, J. S.; PÁDUA, J. G.; PAPPAS JUNIOR, G. J.; GRATTAPAGLIA, D. Utilização de microssatélites derivados de est para a construção de mapas genéticos em cruzamentos intra e interespecíficos de Eucalyptus. In: CONGRESSO BRASILEIRO DE GENÉTICA, 52., 2006, Foz do Iguaçu, PR. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. p. 1148.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | EMEDIATO, F. L.; PASSOS, M. A. N.; TEIXEIRA, C. de C.; PAPPAS JUNIOR, G. J.; MILLER, R. N. G. Análise de expressão de genes análogos de resistência em musa acuminata durante as interações compatíveis e incompatíveis com Mycosphaerella musicola. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 45., 2012, Manaus. Fito 2012. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. Suplemento. Resumo 708. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 99 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
04/09/2015 |
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Data da última atualização: |
16/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. |
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Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, fev., 2015. |
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DOI: |
10.1371/journal.pone.0118231 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. MenosSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST d... Mostrar Tudo |
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Thesagro: |
Broca Gigante; Controle Biológico; Praga. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129096/1/journal.pone.0118231.pdf
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Marc: |
LEADER 02579naa a2200289 a 4500
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008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0118231$2DOI
100 1 $aFONSECA, F. C. de A.
245 $aSugarcane giant borer transcriptome analysis and identification of genes related to digestion.$h[electronic resource]
260 $c2015
520 $aSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control.
650 $aBroca Gigante
650 $aControle Biológico
650 $aPraga
700 1 $aFIRMINO, A. A. P.
700 1 $aMACEDO. L. L. P. de
700 1 $aCOELHO, R. R.
700 1 $aSOUSA JÚNIOR, J. D. A. de
700 1 $aSILVA JUNIOR, O. B.
700 1 $aTOGAWA, R. C.
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aGÓIS, L. A. B. de
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
773 $tPlos One, fev., 2015.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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