Data/hora: 14/06/2024 05:42:29

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Data corrente: 	04/12/1996	
Data da última atualização: 	23/06/2021	
Autoria: 	PANEPUCCI, L.; SCHWANTES, M. L.; SCHWANTES, A. R.	
Afiliação: 	LUCIA PANEPUCCI, EMBRAPA-UEPAE de São Carlos, São Carlos, SP.; M. L. SCHWANTES, UFSCAR; A. R. SCHWANTES, UFSCAR.	
Título: 	Biochemical and physiological properties of the lactate Dehydrogenase allozymes of the Brazilian teleost, Leporinus friderici, anostomidae, cypriniformes.	
Ano de publicação: 	1987	
Fonte/Imprenta: 	Comp. Biochem. Physiol., v.87B, n.1, p.199-206, 1987.	
Idioma: 	Inglês	
Conteúdo: 	-l. Lactate dehydrogenase polymorphism of Leporinusfriderici is determined by two loci, each one encoded by two alleles--Ldh-A, Ldh-A' and Ldh-B and Ldh-B'. 2. Biochemical characterization of the different LDH phenotypes was carded out. Substrate saturation curves show that extracts, where the LDH-B predominates, are much more sensitive to substrate inhibition and have higher thermostability than skeletal muscle extracts where the LDH-A predominates. 3. Pyruvate affinity parameters of the different LDH-B phenotypes show differential values in all temperatures assayed. LDH-BB phenotypes presented always greater affinity values than the LDH-BB' and B'B' ones. 4. The different LDH-B phenotypes show differential thermostability when tested at 65°C, so, the LDH-B'B' phenotype had higher stability than the LDH-BB' and LDH-BB phenotypes. This was corroborated with the isozymes partially purified. 5. A variation was detected in the LDH-B phenotype frequencies since the experiment was started in 1980. 6. All these features suggest that this polymorphism may have an adaptive significance in relation to environmental variability, i.e. temperature fluctuations and water pollution due to industrial waste that notably increased in these last years.	
Palavras-Chave: 	Biochemical; Physiological properties; Propriedade fisiológicas.	
Thesagro: 	Bioquímica.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (11308-1) - UPC - AP - DD - PROCI-1987.00013 - PAN - 1987.00013 - DIS