| |
|
|
| Registros recuperados : 92 | |
| 41. |  | MENEGUCCI, J. L. P.; PAIVA, L. V.; MARINHO, C. S.; AMARAL; A. M.; SOUTO, R. F.; SOUZA, M. de. Influencia da adubacao verde com Crotalaria juncea nas caracteristicas quimicas do solo na camada de 20 a 40cm de profundidade. In: CONGRESSO DA POS-GRADUACAO NA ESAL, 6., 1993, Lavras. Anais: Cerrado: torto, nem por isso errado. Lavras: ESAL, 1993. p.265-266.| Biblioteca(s): Embrapa Cerrados. |
|   |
| 42. | | WAALWIJK, C.; DITA, M. A.; BUDDENHAGEN, I.; PAIVA, L. V. K.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Development of a detection method for tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p.38.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 43. | | PAIVA, L. V.; MARINHO, C. S.; MENEGUCCI, J. L. de P.; AMARAL, M. M. do; SOUZA, M. de. Correlacao entre a diagnose visual e o declinio dos citros. In: CONGRESSO DA POS-GRADUACAO NA ESAL, 6., 1993, Lavras. Anais: Cerrado: torto, nem por isso errado. Lavras: ESAL, 1993. p.195-196.| Biblioteca(s): Embrapa Cerrados. |
| |
| 46. | | COSTA, M. L. M.; LANA, U. G. P.; BARROS, E. C.; PAIVA, L. V.; VALICENTE, F. H. Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda. Journal of Agricultural Science, Cambridge, v. 6, n. 7, p. 1-10, 2014.| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 47. | | NOGUEIRA, D. G.; MALUF, W. R.; NOGUEIRA, D. W.; MACIEL, G. M.; PAIVA, L. V.; FIGUEIRA, A. dos R. Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 4, p. 412-419, abril 2011 Título em inglês: Microsatelite marker associated with the Ty?1 allele for resistance to Begomovirus in tomato.| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
| |
| 48. | | BARRIOS, S. C. L.; TEIXEIRA, J. E.; AZEVEDO, S. M. de; CAMPOS, M. de A.; PAIVA, L. V. Obtenção de anticorpos policlonais contra proteínas presentes em plantas afetadas pela anomalia declínio dos citros. Ciência e Agrotecnologia, Lavras, v. 30, n. 5, p.1013-1016, set./out. 2006.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 49. | | SÁGIO, S. A.; LIMA, A. A.; BARRETO, H. G.; CARVALHO, C. H. S. de; PAIVA, L. V.; CHALFUN-JUNIOR, A. Physiological and molecular analyses of early and late Coffea arabica cultivars at different stages of fruit ripening. Acta Physiologiae Plantarum, v. 35, n. 11, p. 3091-3098, 2014.| Biblioteca(s): Embrapa Café. |
|  |
| 52. | | CHAGAS, P. G.; ABREU, C. S.; MOINO JUNIOR, A.; ZANETTI, R.; PAIVA, L. V.; VALICENTE, F. H. Virulência de cepas de Bt sobre operárias de Atta sexdens (Linnaeus, 1758) (Hymenoptera: Formicidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 28., 2022, Fortaleza. Anais... Fortaleza: Sociedade Entomológica do Brasil, 2022. p. 368.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 53. | | COELHO, G. T. C. P.; SOUZA, I. R. P.; CARNEIRO, A. A.; CARNEIRO, N. P.; ALVES, V. M. C.; PAIVA, L. V.; SCHAFFERT, R. E. Aerenchyma formation in phosphorus-stressed maize roots. In: INTERNATIONAL SYMPOSIUM ON PHOSPHORUS DYNAMICS IN THE SOIL-PLANT CONTINUUM, 3., 2006, Uberlândia. Proceedings... Sete Lagoas: Embrapa Milho e Sorgo, 2006. p. 94-95.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 54. | | LOPES, M. J. C.; CAMPOS, M. de A.; PAIVA, L. V.; SÁ, M. E. L. de; ARANTES, N. E.; ZITO, R. K.; GROSSI-DE-SÁ, M. F. Changes in gene expression in resistant soybean genotype during interaction with Meloidogyne javanica. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 142, ref. P363. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Biblioteca(s): Embrapa Soja. |
| |
| 55. | | ALVES, G. S. C.; DECHAMP, E.; FREIRE, L. P.; POT, D.; THIS, D.; PAIVA, L. V.; ANDRADE, A. C.; MARRACCINI, P.; ETIENNE, H. Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea Arabica. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-47.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 56. | | FREITAS, N. C.; BARRETO, H. G.; TORRES, L. F.; FREIRE, L. L.; RODRIGUES, L. A. Z.; DINIZ, L. E. C.; BEIJO, L. A.; PAIVA, L. V. In silico and in vivo analysis of ABI3 and VAL2 genes during somatic embryogenesis of Coffea arabica: competence acquisition and developmental marker genes. Plant Cell, Tissue and Organ Culture, March, 2019.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
| |
| 57. | | LOPES, M. J. C.; CAMPOS, M. de A.; PAIVA, L. V.; SÁ, M. E. L. de; ARANTES, N. E.; SALGADO, S. M. L.; GROSSI-DE-SÁ, M. F. Histopathological aspects of root-knot nematode resistance in soybean genotype. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 224, trab. 409. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro| Biblioteca(s): Embrapa Soja. |
| |
| 58. | | ALVES, G. S. C.; FREIRE, L. P.; HEIMBECK, I. G. R.; VIEIRA, N. G.; VINECKY, F.; MARRACCINI, P.; PAIVA, L. V.; ANDRADE, A. C. Identificação e análise de polimorfismos na região promotora do gene DREB2A em vários genótipos do gênero Coffea. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 59. | | LIVRAMENTO, K. G. do; FREITAS, N. C.; TRINDADE, L. de O. R.; TEIXEIRA, L. G. da S.; PAIVA, L. V.; BORDALLO, P. do N.; DINIZ, L. E. C. Gene expression analysis associated with tissue-specific promoters in Musa spp. Acta Scientiarum. Agronomy, v. 44, e55893, 2022. 9 p.| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Soja; Embrapa Tabuleiros Costeiros. |
| |
| 60. | | VASCONCELOS, M. J. V.; ANTUNES, M. S.; BARDUCHE, D.; PAIVA, L. V.; PAIVA, E.; VALICENTE, F. H.; FIGUEIREDO, J. E. F.; CARVALHO, C. H. S.; LOPES, M. A. Genetic transformation of tropical maize (Zea mays L.) using Agrobacterium and bombardment. In: INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 6., 1998, San Diego. Final program e abstracts guide. New York: SCHERAGO International, 1998. p. 173.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| Registros recuperados : 92 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
19/01/2009 |
|
Data da última atualização: |
24/07/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
|
Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
|
Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
|
Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
|
Thesagro: |
Doença; Fungo. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
|
|
Marc: |
LEADER 02408nam a2200253 a 4500
001 1069938
005 2023-07-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, A. K.
245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource]
260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.
650 $aDoença
650 $aFungo
653 $aCIRAD139A
653 $aCIRAD86
653 $aDsRed
653 $aGFP
700 1 $aDIAZ TRUJILLO, C.
700 1 $aLEE, T. van der
700 1 $aZWIERS, L.-H.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
