|
|
Registro Completo |
Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
Data corrente: |
27/09/2023 |
Data da última atualização: |
27/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA. D. A. da; CARPENTIERI-PIPOLO, V.; BARRETO, T.; ABDELNOOR, R. V.; MARIN, S. R. R.; CARRAO-PANIZZI, M. C. |
Afiliação: |
Instituto Agronômico Campinas; VALERIA CARPENTIERI PIPOLO, CNPT; Syngenta Proteção de Cultivos LTDA; RICARDO VILELA ABDELNOOR, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; MERCEDES CONCÓRDIA CARRÃO-PANIZZI. |
Título: |
Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 3, GMR19145, 2023. |
DOI: |
https://doi.org/10.4238/gmr19145 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality. MenosAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in th... Mostrar Tudo |
Palavras-Chave: |
Inibidor de tripsina; Kunitz Isoenzimas lipoxigenase; Kunitz trypsin inhibitor; Lipoxygenase isozymes; Molecular markers. |
Thesagro: |
Controle de Qualidade; Glycine Max; Marcador Molecular; Semente; Soja. |
Thesaurus Nal: |
Seeds; Soybeans. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156934/1/genetic-removal-trypsin-inhibitor-and-lipoxygenase-isozymes-form-soybean-seeds-glycine-max.pdf
|
Marc: |
LEADER 02807naa a2200337 a 4500 001 2156934 005 2023-09-27 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4238/gmr19145$2DOI 100 1 $aSILVA. D. A. da 245 $aGenetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (Glycine max) by simple sequence repeat marker assisted selection.$h[electronic resource] 260 $c2023 520 $aAbstract: unitz trypsin inhibitor (KTI), which affects protein digestibility and lipoxygenase isozymes, responsible for the off-flavor associated with soy-based foods, are two undesirable factors present in soybean seeds. These unpleasant factors are usually inactivated by heat treatment. However, heat treatment does not completely eliminate these factors; in addition it may decrease protein solubility and may incur extra energy costs. Genetic elimination of these factors could be an alternative to heat treatment. This study aimed to select soybean lines free of KTI and lipoxygenase isozymes in the seeds. The population under study was obtained by crossing the BRS 213 cultivar, which shows low lipoxygenase activity, with BRS 155, a KTI lacking cultivar. F2:3 hybrid populations were selected and analyzed using DNA markers for the identification of recessive alleles that encode the absence of KTI and the three lipoxygenase enzymes (LOX1, LOX2 and LOX3). F2:3 segregating populations were successfully identified with the KTI specific marker with 100% efficiency. However, the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality. 650 $aSeeds 650 $aSoybeans 650 $aControle de Qualidade 650 $aGlycine Max 650 $aMarcador Molecular 650 $aSemente 650 $aSoja 653 $aInibidor de tripsina 653 $aKunitz Isoenzimas lipoxigenase 653 $aKunitz trypsin inhibitor 653 $aLipoxygenase isozymes 653 $aMolecular markers 700 1 $aCARPENTIERI-PIPOLO, V. 700 1 $aBARRETO, T. 700 1 $aABDELNOOR, R. V. 700 1 $aMARIN, S. R. R. 700 1 $aCARRAO-PANIZZI, M. C. 773 $tGenetics and Molecular Research$gv. 22, n. 3, GMR19145, 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Trigo (CNPT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Cocais. Para informações adicionais entre em contato com cpacp.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cocais. |
Data corrente: |
23/11/2011 |
Data da última atualização: |
26/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
ALBUQUERQUE, C. J. B.; FERNANDES, L. de O.; GUIMARÃES, A. de S.; PAES, J. M. V; BRANT, R. da S.; FREITAS, R. S. de. |
Afiliação: |
CARLOS JULIANO BRANT ALBUQUERQUE, Pesquisador EPAMIG; LEONARDO DE OLIVEIRA FERNANDES, Pesquisador EPAMIG; ADRIANO DE SOUZA GUIMARÃES, Pesquisador EPAMIG; JOSÉ MAURO VALENTE PAES, Pesquisador EPAMIG; RENATA DA SILVA BRANT, CPACP; ROGÉRIO SOARES DE FREITAS, Pesquisador IAC/APTA. |
Título: |
Avaliação e escolha de milho para silagem. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Informe Agropecuário, Belo Horizonte, BH, v. 32, n. 260, p. 68-76, jan./fev. 2011. |
Páginas: |
9 p. |
Idioma: |
Português |
Conteúdo: |
Dentre os recursos forrageiros utilizados para a suplementação de ruminantes no período seco de ano, a silagem de milho é a opção mais adotada e difundida entre os produtores, majoritariamente os que dedicam à pecuária de leite. Considerada padrão, a silagem de milho é um volumoso que gera bom desempenho animal. Tal fato deve-se as características qualitativas e quantitativas da planta de milho, as quais promovem boa aceitação dos bovinos, por constituir silagem de alto conteúdo energético, alta produção de matéria seca (MS) por unidade de área e fácil confecção. A correta escolha de híbridos de milho para silagem visa qualidade nutricional. |
Palavras-Chave: |
Milho dentado; Milho duro. |
Thesagro: |
Digestibilidade; Ensilagem; Zea Mays. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01384naa a2200253 a 4500 001 1906931 005 2018-02-26 008 2011 bl uuuu u00u1 u #d 100 1 $aALBUQUERQUE, C. J. B. 245 $aAvaliação e escolha de milho para silagem.$h[electronic resource] 260 $c2011 300 $a9 p. 520 $aDentre os recursos forrageiros utilizados para a suplementação de ruminantes no período seco de ano, a silagem de milho é a opção mais adotada e difundida entre os produtores, majoritariamente os que dedicam à pecuária de leite. Considerada padrão, a silagem de milho é um volumoso que gera bom desempenho animal. Tal fato deve-se as características qualitativas e quantitativas da planta de milho, as quais promovem boa aceitação dos bovinos, por constituir silagem de alto conteúdo energético, alta produção de matéria seca (MS) por unidade de área e fácil confecção. A correta escolha de híbridos de milho para silagem visa qualidade nutricional. 650 $aDigestibilidade 650 $aEnsilagem 650 $aZea Mays 653 $aMilho dentado 653 $aMilho duro 700 1 $aFERNANDES, L. de O. 700 1 $aGUIMARÃES, A. de S. 700 1 $aPAES, J. M. V 700 1 $aBRANT, R. da S. 700 1 $aFREITAS, R. S. de 773 $tInforme Agropecuário, Belo Horizonte, BH$gv. 32, n. 260, p. 68-76, jan./fev. 2011.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Cocais (CPACP) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|