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| 1. |  | NOONAN, M. J.; FLEMING, C. H.; TUCKER, M. A.; KAYS, R.; HARRISON, AUTUMN-LYNN; CROFOOT, M. C.; ABRAHMS, B.; ALBERTS, S.; ALI, A. H.; ALTMANN, J.; ANTUNES, P. C.; ATTIAS, N.; BELANT, J. L.; BEYER JUNIOR, D. E.; BIDNER, L. R.; BLAUM, N.; BOONE, R. B.; CAILLAUD, D.; PAULA, R. C. de; DE LA TORRE, J. A.; DEKKER, J.; DEPERNO, C. S.; FARHADINIA, M.; FENNESSY, J.; FICHTEL, C.; FISCHER, C.; FORD, A.; GOHEEN, J. R.; HAVMØLLER, R. W.; HIRSCH, B. T.; HURTADO, C.; ISBELL, L. A.; JANSSEN, R.; JELTSCH, F.; KACZENSKY, P.; KANEKO, Y.; KAPPELER, P.; KATNA, A.; KAUFFMAN, M.; KOCH, F.; KULKARNI, A; LAPOINT, S.; LEIMGRUBER, P.; MACDONALD, D. W.; MARKHAM, A. C.; MCMAHON, L.; MERTES, K.; MOORMAN, C. E.; MORATO, R. G.; MOßBRUCKER, A. M.; MOURAO, G.; O'CONNOR, D.; OLIVEIRA-SANTOS, L. G. R.; PASTORINI, J.; PATTERSON, B. D.; RACHLOW, J.; RANGLACK, D. H.; REID, N.; SCANTLEBURY, D. M.; SCOTT, D. M.; SELVA, N.; SERGIEL, A.; SONGER, M.; SONGSASEN, N.; STABACH, J. A.; STACY-DAWES, J.; SWINGEN, M. B.; THOMPSON, J. J.; ULLMANN, W.; VANAK, A. T.; THAKER, M.; WILSON, J. W.; YAMAZAKI, K.; YARNELL, R. W.; ZIEBA, F.; ZWIJACZ-KOZICA, T.; FAGAN, W. F.; MUELLER, T.; CALABRESE, J. M. Effects of body size on estimation of mammalian area requirements. Conservation Biology, v.34, n. 4, p. 1017-1028, 2020.| Biblioteca(s): Embrapa Pantanal. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/10/2012 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FIGUEIREDO, J. E. F.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
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Título: |
Simple, rapid and accurate PCR-based detection of Pantoea ananatis in maize, sorghum and Digitaria sp. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Plant Pathology, Pisa, v. 94, n. 3, p. 663-667, 2012. |
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DOI: |
10.4454/JPP.FA.2012.049 |
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Idioma: |
Inglês |
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Conteúdo: |
Detection of Pantoea ananatis in the early growing season is important for disease prediction and management. We developed a simple molecular marker based on PCR for conclusive diagnosis of P. ananatisin maize, sorghum and Digitaria sp. A pair of primers was used for amplifying only one of the seven internal transcribed spacer (ITS) regions of P. ananatis 16S-23S rRNA genes. Sixty-three strains of P. ananati from diverse ecogeographical origins; total DNA of pool of maize white spot (MWS) lesions and MWS-like lesions from sorghum and Digitaria sp.; reference strains of P. agglomerans, P. ananatis, P. stewartii, P. allii, P. vagans, P.anthophila, P. eucalypti, P. deleyi, P. rodasii, P. rwandensis and P. wallisii were used for testing the specificity of the primers. A single amplicon per sample, 361 bp or 389 bp in size, was obtained from P. ananatis from different sources and P. allii isolated from Allium cepa and the identity of all amplicons was confirmed by DNA sequencing. The present results provide a rapid and reliable tool for the accurate identification of P. ananatis isolates and for direct PCR-based diagnosis of P. ananatis associated with maize, sorghum and Digitaria sp. |
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Thesagro: |
Milho; Sorghum bicolor; Sorgo; Zea mays. |
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Thesaurus NAL: |
Plant pathogens. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01837naa a2200205 a 4500
001 1936679
005 2018-05-28
008 2012 bl uuuu u00u1 u #d
024 7 $a10.4454/JPP.FA.2012.049$2DOI
100 1 $aFIGUEIREDO, J. E. F.
245 $aSimple, rapid and accurate PCR-based detection of Pantoea ananatis in maize, sorghum and Digitaria sp.$h[electronic resource]
260 $c2012
520 $aDetection of Pantoea ananatis in the early growing season is important for disease prediction and management. We developed a simple molecular marker based on PCR for conclusive diagnosis of P. ananatisin maize, sorghum and Digitaria sp. A pair of primers was used for amplifying only one of the seven internal transcribed spacer (ITS) regions of P. ananatis 16S-23S rRNA genes. Sixty-three strains of P. ananati from diverse ecogeographical origins; total DNA of pool of maize white spot (MWS) lesions and MWS-like lesions from sorghum and Digitaria sp.; reference strains of P. agglomerans, P. ananatis, P. stewartii, P. allii, P. vagans, P.anthophila, P. eucalypti, P. deleyi, P. rodasii, P. rwandensis and P. wallisii were used for testing the specificity of the primers. A single amplicon per sample, 361 bp or 389 bp in size, was obtained from P. ananatis from different sources and P. allii isolated from Allium cepa and the identity of all amplicons was confirmed by DNA sequencing. The present results provide a rapid and reliable tool for the accurate identification of P. ananatis isolates and for direct PCR-based diagnosis of P. ananatis associated with maize, sorghum and Digitaria sp.
650 $aPlant pathogens
650 $aMilho
650 $aSorghum bicolor
650 $aSorgo
650 $aZea mays
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tJournal of Plant Pathology, Pisa$gv. 94, n. 3, p. 663-667, 2012.
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