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| Registros recuperados : 26 | |
| 14. |  | FRANCISCON, L.; RIBEIRO JUNIOR, P. J.; KRAINSKI, E. T.; BASSANEZI, R. B.; CZERMAINSKI, A. B. C. Modelo autologístico espaço-temporal com aplicação à análise de padrões espaciais da Leprose-dos-citros. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 12, p. 1677-1682, 2008.| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| 15. | | RIBEIRO JÚNIOR, P. J.; VIOLA, D. N.; DEMÉTRIO, C. G. B.; MANLY, B. F.; FERNANDES, O. A. Spatial pattern detection modeling thrips (Thrips tabaci) on onion fields. Scientia Agricola, Piracicaba, v. 66, n. 1, p. 90-99, jan./fev. 2009.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | WOJCIECHOWSKI, J. C.; ARCE, J. E.; WEBER, S. H.; RIBEIRO JÚNIOR, P. J.; PIRES, C. A. da F. Log-verossimilhança combinada para comparação de estruturas de continualidade espacial em floresta estacional decidual. Floresta, Curitiba, v. 45, n. 4, p. 797-808, out./dez. 2015.| Biblioteca(s): Embrapa Florestas. |
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| 17. | | GREGO, S.; GREGO, C. R.; RIBEIRO JUNIOR, P. J.; VAZ, C. M. P.; HURTADO, S.; RODRIGUES, K. DE M. Densidade do solo de um Nitrossolo sob cultivo de cana-de-açúcar utilzando analises geoestatística e bayesiana. In: SIMPÓSIO DE GEOESTATÍSTICA APLICADA EM CIÊNCIAS AGRÁRIAS, 3., 2013, Botucatu, SP. Anais... Botucatu, SP: 4 p.| Biblioteca(s): Embrapa Territorial. |
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| 19. | | BOGNOLA, I. A.; RIBEIRO JÚNIOR, P. J.; SILVA, E. A. A. da; LINGNAU, C.; HIGA, A. R. Modelagem uni e bivariada da variabilidade espacial de rendimento de Pinus taeda L. Floresta, Curitiba, v. 38, n. 2, p. 373-385, abr./jun. 2008.| Biblioteca(s): Embrapa Florestas. |
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| 20. | | SAUTTER, K. D.; MOTTA NETO, J. A.; MORAES, A. de; SANTOS, H. R. dos; RIBEIRO JUNIOR, P. J. População de Oribatei e Collembola em pastagens na recuperação de solos degradados pela mineração do xisto. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 9, p. 1509-13, set. 1998. Título em inglês: Population of Oribatei and Collembola in pastures for reclamation of degraded lands in a schist mine.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 26 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/06/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
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Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
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DOI: |
10.1590/S1982-56762008000100010 |
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Idioma: |
Inglês |
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Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
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Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
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Thesagro: |
Milho; Zea Mays. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
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Marc: |
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001 1491172
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008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S1982-56762008000100010$2DOI
100 1 $aBOMFETI, C. A.
245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource]
260 $c2008
520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
650 $aMilho
650 $aZea Mays
653 $aDoença bacteriana do milho
653 $adoenças foliares do milho
653 $aMaize bacterial disease
653 $aMaize leaf disease
700 1 $aSOUZA-PACCOLA, E. A.
700 1 $aMASSOLA JUNIOR, N. S.
700 1 $aMARRIEL, I. E.
700 1 $aMEIRELLES, W. F.
700 1 $aCASELA, C. R.
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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