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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
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Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
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Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Santa ines. |
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Thesagro: |
Ovis Aries. |
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Thesaurus Nal: |
cryopreservation; gene expression. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02970naa a2200289 a 4500
001 2085659
005 2023-01-27
008 2018 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource]
260 $c2018
520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $acryopreservation
650 $agene expression
650 $aOvis Aries
653 $aIn vivo embryo production
653 $aSanta ines
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDAO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
16/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
AIRES, E. S.; ARAGÃO, C. A.; DANTAS, B. F.; RODRIGUES, J. D.; ONO, E. O. |
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Afiliação: |
EDUARDO SANTANA AIRES; CARLOS ALBERTO ARAGÃO; BARBARA FRANCA DANTAS, CPATSA; JOÃO DOMINGOS RODRIGUES; ELIZABETH ORIKA ONO. |
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Título: |
Light intensity modulates the accumulation of carbohydrates, antioxidant enzymes and production of iceberg lettuce under tropical conditions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Horticulturae, v. 7, n. 12, 553, 2021. |
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DOI: |
https://doi.org/10.3390/horticulturae7120553 |
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Idioma: |
Inglês |
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Conteúdo: |
High solar radiation in tropical regions can affect the development, physiology, and biochemistry of plants. Our aim with this research was to evaluate the biochemical responses and production of iceberg lettuce cultivars under environments with different shadings. An experiment under field conditions was installed in a 4 × 4 factorial scheme. Four cultivars of iceberg lettuce (Great Lakes, Winslow, Delicia, and Balsamo) and four environments with different shadings (three types of shading screens: red, ChromatiNet®, and black; and treatment under the full sun) were evaluated. Forty-five days after transplanting, the plants were harvested, and the biomass was weighed to obtain the fresh consumable part (FCP) and the biochemical parameters: total soluble sugar (TSS), reducing sugar (RS), and the activity of SOD, CAT, and POD. We found that shading modulates biochemical and productive responses of iceberg lettuce, and the main microclimatic factor related to these responses was solar radiation. We observed a negative correlation between RS and FCP. The solar radiation between 16 and 18 MJ·m−2 ·day−1 , observed in the environments with a black screen and ChromatiNet®, promoted the highest activity of the SOD enzyme and average levels of TSS and RS, providing higher FCP of the cultivars Delícia and Balsamo in high temperatures period. |
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Palavras-Chave: |
Alface americana; Aparafusamento. |
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Thesagro: |
Açúcares; Alface; Fisiologia; Lactuca Sativa; Sombreamento. |
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Thesaurus NAL: |
Lettuce. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02207naa a2200277 a 4500
001 2137518
005 2021-12-16
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/horticulturae7120553$2DOI
100 1 $aAIRES, E. S.
245 $aLight intensity modulates the accumulation of carbohydrates, antioxidant enzymes and production of iceberg lettuce under tropical conditions.$h[electronic resource]
260 $c2021
520 $aHigh solar radiation in tropical regions can affect the development, physiology, and biochemistry of plants. Our aim with this research was to evaluate the biochemical responses and production of iceberg lettuce cultivars under environments with different shadings. An experiment under field conditions was installed in a 4 × 4 factorial scheme. Four cultivars of iceberg lettuce (Great Lakes, Winslow, Delicia, and Balsamo) and four environments with different shadings (three types of shading screens: red, ChromatiNet®, and black; and treatment under the full sun) were evaluated. Forty-five days after transplanting, the plants were harvested, and the biomass was weighed to obtain the fresh consumable part (FCP) and the biochemical parameters: total soluble sugar (TSS), reducing sugar (RS), and the activity of SOD, CAT, and POD. We found that shading modulates biochemical and productive responses of iceberg lettuce, and the main microclimatic factor related to these responses was solar radiation. We observed a negative correlation between RS and FCP. The solar radiation between 16 and 18 MJ·m−2 ·day−1 , observed in the environments with a black screen and ChromatiNet®, promoted the highest activity of the SOD enzyme and average levels of TSS and RS, providing higher FCP of the cultivars Delícia and Balsamo in high temperatures period.
650 $aLettuce
650 $aAçúcares
650 $aAlface
650 $aFisiologia
650 $aLactuca Sativa
650 $aSombreamento
653 $aAlface americana
653 $aAparafusamento
700 1 $aARAGÃO, C. A.
700 1 $aDANTAS, B. F.
700 1 $aRODRIGUES, J. D.
700 1 $aONO, E. O.
773 $tHorticulturae$gv. 7, n. 12, 553, 2021.
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