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| Registros recuperados : 116 | |
| 62. |  | PEREIRA, G. G.; GOLDFEDER, G. T.; YAMAKI, F. L.; OLIVEIRA, V. M. C.; LARSSON, M. H. M. A. Myocardial velocities obtained by pulsed tissue Doppler in English Cocker Spaniels with dilated cardiomyopathy and congestive heart failure. Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 851-856, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 63. | | VALARINI, M. J.; BROCHINI, D.; FANTINATTI, F.; OLIVEIRA, V. M.; MANFIO, G. P.; MELO, I. S. de. Bactérias diazotróficas endofíticas isoladas de espécies de Brachiaria. In: SIMPÓSIO, 4., REUNIÃO DE AVALIAÇÃO DO PROGRAMA BIOTA/FAPESP, 4., Águas de Lindóia, 2003. Anais... Águas de Lindóia: FAPESP, 2003.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 69. | | OLIVEIRA, V. M.; FONSECA, A. H.; PEREIRA, M. J. S.; CARNEIRO, A. V.; JESUS, V. L. T.; ALVES, P. A. M. Análise retrospectiva dos fatores associados à distribuição da tuberculose bovina no Estado do Rio de Janeiro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 60, n. 3, p. 574-579, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 71. | | OLIVEIRA, V. M. de; MENDONCA, L. C.; MIRANDA, J. E. C. de; DINIZ, F. H.; REIS, E. S. dos; GUIMARAES, A. S.; MAGALHAES, V. M. A. de. Como identificar a vaca com mastite em sua propriedade: cartilhas elaboradas conforme a metodologia e-Rural. Brasília, DF: Embrapa, 2016. Ebook : il. color. Ebook no formato epub, convertido do livro impresso.| Biblioteca(s): Embrapa Gado de Leite. |
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| 72. | | CARNEIRO, A. V.; STOCK, L. A.; OLIVEIRA, V. M.; ZOCCAL, R.; CARVALHO, G. R.; MARTINS, P. do C.; YAMAGUCHI, L. C. T. Mastite clínica: prevalência e custo de tratamento em rebanho leiteiro. In: REUNIÓN ASOCIACÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 20., REUNIÓN ASOCIACIÓN PERUANA DE PRODUCCIÓN ANIMAL, 30., CONGRESO INTERNACIONAL DE GANADERIA DOBLE PROPOSITO, 5., 2007, Cuzco. Anais... Cuzco: ALPA/APPA, 2007. 1 CD.| Biblioteca(s): Embrapa Gado de Leite. |
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| 73. | | CARNEIRO, A. V.; STOCK, L. A.; OLIVEIRA, V. M.; ZOCCAL, R.; CARVALHO, G. R.; MARTINS, P. do C.; YAMAGUCHI, L. C. T. Mastite clínica: prevalência e custo de tratamento em rebanho leiteiro. In: REUNIÓN ASOCIACÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 20.; REUNIÓN ASOCIACIÓN PERUANA DE PRODUCCIÓN ANIMAL, 30.; CONGRESO INTERNACIONAL DE GANADERIA DOBLE PROPOSITO, 5., 2007, Cuzco. Resumenes... Cuzco: ALPA/APPA, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 74. | | OTTONI, J. R.; BELOTI, L. L.; SANTIAGO, A. S.; COSTA, B. Z.; MELO, I. S. de; SOUZA, A. P.; MARSAIOLI, A. J.; OLIVEIRA, V. M. Molecular cloning and functional characterization of a catalase gene from a Bacillus safensis strain isolated from an oil-impacted Brazilian mangrove sediment. Hechos Microbiológicos, v. 5, n. 2, p. 29, 2014. Suplemento. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014. Ref. TLO-7.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 75. | | SILVA, M. R.; GUIMARAES, M. D. C.; OLIVEIRA, V. M. de; COSTA, R. R.; ABI-ZAID, K. C. F.; ARAUJO, F. R.; ROCHA, A. S.; SUFFYS, P. N. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyP pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 56| Biblioteca(s): Embrapa Gado de Leite. |
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| 76. | | COSTA, R. R.; ROCHA, A. S.; MOREIRA, A. S.; ABI-ZAID, K. C. F.; SILVA, M. R.; GUIMARAES, M. D. C.; SUFFYS, P. N.; OLIVEIRA, V. M. Identificação do complexo Mycobacterium tuberculosis com base na amplificação e sequenciamento do gene oxyr de esfregaços de escarro corados por ziehl-neelsen arquivados no Brasil. Revista Médica de Minas Gerais, v. 22, supl. 2, p. S129, 2012. Edição dos resumos do 3º Fórum Científico da Fundação Hospitalar do Estado de Minas Gerais, Juiz de Fora, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 77. | | CARNEIRO, A. V.; BRITO, J. R. F.; FONSECA, A. H.; STOCK, L. A.; OLIVEIRA, V. M.; ZOCCAL, R.; YAMAGUCHI, L. C. T. Impacto econômico da mastite bovina em rebanhos leiteiros. In: CONGRESSO INTERNACIONAL DO LEITE, 6., 2007, Resende. Anais... Juiz de Fora: Embrapa Gado de Leite, 2007. 1 CD.| Biblioteca(s): Embrapa Gado de Leite. |
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| 78. | | DIAMANTINO, G. M. L.; PIEREZAN, F.; FERREIRA, M. I. C.; ROCHA, W. S. D. da; OLIVEIRA, V. M. de; MARTINS, C. E.; VEIGA, M. O.; SOTO-BLANCO, B. Photosensitization by Brachiaria ruziziensis in a sheep herd. Toxicon, v. 185, p. 1-4, 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 79. | | OLIVEIRA, V. M. de; YAMAGUCHI, L. C. T.; CARNEIRO, A. V.; MORAIS, R. J. da S.; BRITO NETO, E. P. de. Brucelose bovina: aspectos técnicos e econômicos. In: YAMAGUCHI, L. C. T.; MENDES, L. C. R.; LIMA, I. B. de; RODRIGUES, C. do C.; RESENDE, M. L. de. (Ed.). Aspectos sócio-econômicos e ambientais da produção de leite. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 175-186.| Biblioteca(s): Embrapa Gado de Leite. |
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| 80. | | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; SOBRAL, B. W. S.; GUIMARAES, C. T.; ELSAS, J. D. van; MANFIO, G. P. Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE). Letters in Applied Microbiology, v. 28, N. 2, p. 137-141, Feb. 1999.| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Solos. |
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| Registros recuperados : 116 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. J.; DUARTE, A. W. F.; ROSA, L. H.; OLIVEIRA, V. M.; MELO, I. S. de. |
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Afiliação: |
L. J. SILVA, ESALQ/USP; A. W. F. DUARTE, USP; L. H. ROSA, UFMG; V. M. OLIVEIRA, CPQBA/Unicamp. |
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Título: |
Fosmid library: methodological challenges. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest. MenosAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A p... Mostrar Tudo |
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Palavras-Chave: |
Antarctic peninsula; Metagenomic library; Unculturable microrganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139515/1/2015RA-008.pdf
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Marc: |
LEADER 03101nam a2200193 a 4500
001 2037800
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, L. J.
245 $aFosmid library$bmethodological challenges.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1.$c2015
520 $aAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest.
653 $aAntarctic peninsula
653 $aMetagenomic library
653 $aUnculturable microrganisms
700 1 $aDUARTE, A. W. F.
700 1 $aROSA, L. H.
700 1 $aOLIVEIRA, V. M.
700 1 $aMELO, I. S. de
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