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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Amapá; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte. |
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Data corrente: |
31/03/1999 |
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Data da última atualização: |
10/01/2024 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
GIRAO, R. N.; NASCIMENTO, H. T. S. do; LEAL, J. A.; NASCIMENTO, M. do P. S. C. B. |
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Afiliação: |
RAIMUNDO NONATO GIRAO, CPAMN; HOSTON TOMAS SANTOS DO NASCIMENTO, CPAMN; JOSE ALCIMAR LEAL, CPAMN; MARIA DO PERPETUO S C B NASCIMENTO, CPAMN. |
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Título: |
Suplementação alimentar para vacas no final da gestação no período seco. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Teresina: Embrapa Meio-Norte, 1998. |
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Páginas: |
4p. |
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Série: |
(Embrapa Meio-Norte. Pesquisa em Andamento, 79). |
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Idioma: |
Português |
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Palavras-Chave: |
Feed suplements; Gestação; Periodo seco; Piaui; Suplementacao alimentar; Supplements; Vacas. |
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Thesagro: |
Bovino; Nutrição Animal; Suplemento Alimentar; Vaca. |
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Thesaurus Nal: |
animal nutrition; cows; dry season; pregnancy. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97563/1/PAND790001.pdf
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Marc: |
LEADER 00917nam a2200337 a 4500
001 1055072
005 2024-01-10
008 1998 bl uuuu u0uu1 u #d
100 1 $aGIRAO, R. N.
245 $aSuplementação alimentar para vacas no final da gestação no período seco.
260 $aTeresina: Embrapa Meio-Norte$c1998
300 $a4p.
490 $a(Embrapa Meio-Norte. Pesquisa em Andamento, 79).
650 $aanimal nutrition
650 $acows
650 $adry season
650 $apregnancy
650 $aBovino
650 $aNutrição Animal
650 $aSuplemento Alimentar
650 $aVaca
653 $aFeed suplements
653 $aGestação
653 $aPeriodo seco
653 $aPiaui
653 $aSuplementacao alimentar
653 $aSupplements
653 $aVacas
700 1 $aNASCIMENTO, H. T. S. do
700 1 $aLEAL, J. A.
700 1 $aNASCIMENTO, M. do P. S. C. B.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
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Título: |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n.3, 2019. |
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Idioma: |
Inglês |
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Notas: |
gmr18394 |
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Conteúdo: |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
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Thesagro: |
Melhoramento Vegetal. |
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Thesaurus NAL: |
Breeding and Genetic Improvement. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202165/1/-rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill.pdf
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Marc: |
LEADER 02135naa a2200289 a 4500
001 2112332
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aRapid plant DNA and RNA extraction protocol using a bench drill Brief Note.$h[electronic resource]
260 $c2019
500 $agmr18394
520 $aPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
650 $aBreeding and Genetic Improvement
650 $aMelhoramento Vegetal
700 1 $aGUTIERREZ, D. L.
700 1 $aKREUZE, J. F.
700 1 $aISKRA-CARUANA, M. L.
700 1 $aCHABANNES, M.
700 1 $aBARBOSA, A. C. O.
700 1 $aSANTOS, T. A.
700 1 $aSILVA, A. G. S.
700 1 $aSANTOS, R. M. F.
700 1 $aAMORIM, E. P.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aJESUS, O. N. de
773 $tGenetics and Molecular Research$gv. 18, n.3, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
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