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| 7. |  | MAY-DE-MIO, L. L.; MOREIRA, L. M; MONTEIRO, L. B.; JUSTINIANO JUNIOR, P. R. Infecção de Monilinia fructicola no período da floração e incidência de podridão parda em frutos de pessegueiro em dois sistemas de produção. Tropical Plant Pathology, Brasília, DF, v. 33, n. 3, p. 227-234, 2008.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | LEMÕES, J. S.; MOREIRA, L. M.; KNEIB, R. B.; SILVA, S. D. dos A. e.; CASAGRANDE JUNIOR, J. G. Comparação entre protocolos de extração de DNA de pinhão manso. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 2.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 2., 2008, Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | SILVA, U. C.; SILVA, D. R. C. da; LEITE, L. R.; FELESTRINO, E. B.; MOREIRA, L. M.; ALMEIDA JÚNIOR, N. F.; OLIVEIRA-PAIVA, C. A.; SANTOS, V. L. Predições guiadas por genômica do potencial de promoção de crescimento vegetal de isolados de Serratia marcescens endofíticos de milho (Zea mays). In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 4., 2017, Belo Horizonte. Metabolismo microbiano: saúde, ambiente e biotecnologia: resumos. Belo Horizonte: UFMG, 2017. p. 191.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | SILVA, U. de C.; SILVA, D. R. C.; LEITE, L. R.; MEDEIROS, L. D.; FELESTRINO, E. B.; CANESCHI, W. L.; MOREIRA, L. M.; ALMEIDA JÚNIOR, N.; SILVA, R.; OLIVEIRA-PAIVA, C. A.; SANTOS, V. L. dos. Genômica comparativa de Serratia marcescens endofíticas isoladas de Zea mays com linhagens de Serratia de diferentes estilos de vida . In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24.; CONGRESO CHILENO DE MICROBIOLOGIA, 40.; REUNIÓN ANUAL DE LA ASSOCIACIÓN CHILENA DE INMUNOLOGIA, 2.; REUNIÓN DE LA SOCIEDAD LATINOAMERICANA DE TUBERCULOSIS Y OTRAS MICOBACTERIOSIS, 9., 2018, Santiago, Chile. Libro de resúmenes. Santiago: Asociación Latinoamericana de Microbiología, 2018. p. 785.| Biblioteca(s): Embrapa Milho e Sorgo. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
05/02/2019 |
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Data da última atualização: |
21/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ROCHA, M. I. P.; SOUZA, M. M. de; ZERLOTINI NETO, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; AFONSO, J.; DINIZ, W. J. da S.; BUSS, C. E.; COUTINHO, L. L.; REGITANO, L. C. de A.; NICIURA, S. C. M. |
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Afiliação: |
MARINA IBELLI PEREIRA ROCHA, UFSCar; MARCELA MARIA DE SOUZA, UFSCar; ADHEMAR ZERLOTINI NETO, CNPTIA; POLYANA CRISTINE TIZIOTO, Esalq/USP; PRISCILA SILVA NEUBERN DE OLIVEIRA, Bolsista CPPSE; ANDRESSA OLIVEIRA DE LIMA, UFSCar; JULIANA AFONSO, UFSCar; WELLISON JARLES DA SILVA DINIZ, UFSCar; CARLOS EDUARDO BUSS, UFSCar; LUIZ LEHMANN COUTINHO, Esalq/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE. |
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Título: |
LDHB gene has allele-specific expression in liver of Nelore cattle extremes for feed efficiency. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: WORKSHOP ON OMICS STRATEGIES APPLIED TO LIVESTOCK SCIENCE, 1., 2017, Piracicaba, SP. Proceedings... São Carlos, SP: Embrapa Pecuária Sudeste, 2017. |
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Páginas: |
p. 25. |
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Série: |
(Embrapa Pecuária Sudeste. Documentos, 125). |
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ISSN: |
1980-6841 |
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Idioma: |
Inglês |
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Notas: |
Editores: Luiz Lehmann Coutinho, Luciana C. de A. Regitano, Gerson Barreto Mourão, Aline Silva Mello Cesar, Bárbara Silva Vignato, Mirele Daiana Poleti, Wellison Jarles da Silva Diniz. |
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Conteúdo: |
Feed efficiency is a multi-factorial trait of a large economic importance for cattle. Although previous studies reported gene expression differences associated with this trait the contribution of allele-specific expression (ASE) remains largely unknown. In this study, we analyzed ASE in liver samples of 30 Nelore cattle steers in two genetically divergent groups for residual feed intake (RFI) searching for genes with ASE linked to feed efficiency. Based on genotype data obtained using the Illumina BovineHD BeadChip, we computed the frequency of reads from RNA-seq data mapped to each allele of heterozygous individuals and applied a binomial test to identify loci of ASE. We detected significant differences in expression among alleles for seven SNPs (single nucleotide polymorphisms) that were tested significantly in >90% of the samples. Amid them, we selected the LDHB gene that has been previously associated with feed efficiency in chickens. The LDHB gene carries out functions in carbohydrate, carboxylic acid and oxidation-reduction metabolic processes important for glycolysis. These biological processes were associated with feed efficiency and cell energy balance in previous studies. Since the SNP found on LDHB is located at 3?UTR, we studied whether a putative microRNA binding site near or at the SNP site could
exist and account for the regulation of gene expression. We found that the Bta-miR-139 binds at the SNP site of LDHB. This miRNA was also identified by the group in a previous study with expressed microRNA in the liver. Thus, we theorized that the BtamiR-139 miRNA affects the expression of the LDHB gene and may contribute to ASE. Therefore, these results complement our understanding of the ASE profile of the LDHB gene and contribute to explain more accurately the differences in gene expression identified in Nelore steers genetically divergent for RFI. MenosFeed efficiency is a multi-factorial trait of a large economic importance for cattle. Although previous studies reported gene expression differences associated with this trait the contribution of allele-specific expression (ASE) remains largely unknown. In this study, we analyzed ASE in liver samples of 30 Nelore cattle steers in two genetically divergent groups for residual feed intake (RFI) searching for genes with ASE linked to feed efficiency. Based on genotype data obtained using the Illumina BovineHD BeadChip, we computed the frequency of reads from RNA-seq data mapped to each allele of heterozygous individuals and applied a binomial test to identify loci of ASE. We detected significant differences in expression among alleles for seven SNPs (single nucleotide polymorphisms) that were tested significantly in >90% of the samples. Amid them, we selected the LDHB gene that has been previously associated with feed efficiency in chickens. The LDHB gene carries out functions in carbohydrate, carboxylic acid and oxidation-reduction metabolic processes important for glycolysis. These biological processes were associated with feed efficiency and cell energy balance in previous studies. Since the SNP found on LDHB is located at 3?UTR, we studied whether a putative microRNA binding site near or at the SNP site could
exist and account for the regulation of gene expression. We found that the Bta-miR-139 binds at the SNP site of LDHB. This miRNA was also identified by the group in a ... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Residual feed intake. |
|
Thesagro: |
Gado de Corte. |
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Thesaurus NAL: |
cattle; microRNA. |
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Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 03197nam a2200349 a 4500
001 2105559
005 2020-01-21
008 2017 bl uuuu u01u1 u #d
022 $a1980-6841
100 1 $aROCHA, M. I. P.
245 $aLDHB gene has allele-specific expression in liver of Nelore cattle extremes for feed efficiency.$h[electronic resource]
260 $aIn: WORKSHOP ON OMICS STRATEGIES APPLIED TO LIVESTOCK SCIENCE, 1., 2017, Piracicaba, SP. Proceedings... São Carlos, SP: Embrapa Pecuária Sudeste$c2017
300 $ap. 25.
490 $a(Embrapa Pecuária Sudeste. Documentos, 125).
500 $aEditores: Luiz Lehmann Coutinho, Luciana C. de A. Regitano, Gerson Barreto Mourão, Aline Silva Mello Cesar, Bárbara Silva Vignato, Mirele Daiana Poleti, Wellison Jarles da Silva Diniz.
520 $aFeed efficiency is a multi-factorial trait of a large economic importance for cattle. Although previous studies reported gene expression differences associated with this trait the contribution of allele-specific expression (ASE) remains largely unknown. In this study, we analyzed ASE in liver samples of 30 Nelore cattle steers in two genetically divergent groups for residual feed intake (RFI) searching for genes with ASE linked to feed efficiency. Based on genotype data obtained using the Illumina BovineHD BeadChip, we computed the frequency of reads from RNA-seq data mapped to each allele of heterozygous individuals and applied a binomial test to identify loci of ASE. We detected significant differences in expression among alleles for seven SNPs (single nucleotide polymorphisms) that were tested significantly in >90% of the samples. Amid them, we selected the LDHB gene that has been previously associated with feed efficiency in chickens. The LDHB gene carries out functions in carbohydrate, carboxylic acid and oxidation-reduction metabolic processes important for glycolysis. These biological processes were associated with feed efficiency and cell energy balance in previous studies. Since the SNP found on LDHB is located at 3?UTR, we studied whether a putative microRNA binding site near or at the SNP site could exist and account for the regulation of gene expression. We found that the Bta-miR-139 binds at the SNP site of LDHB. This miRNA was also identified by the group in a previous study with expressed microRNA in the liver. Thus, we theorized that the BtamiR-139 miRNA affects the expression of the LDHB gene and may contribute to ASE. Therefore, these results complement our understanding of the ASE profile of the LDHB gene and contribute to explain more accurately the differences in gene expression identified in Nelore steers genetically divergent for RFI.
650 $acattle
650 $amicroRNA
650 $aGado de Corte
653 $aDifferential expression
653 $aResidual feed intake
700 1 $aSOUZA, M. M. de
700 1 $aZERLOTINI NETO, A.
700 1 $aTIZIOTO, P. C.
700 1 $aOLIVEIRA, P. S. N. de
700 1 $aLIMA, A. O. de
700 1 $aAFONSO, J.
700 1 $aDINIZ, W. J. da S.
700 1 $aBUSS, C. E.
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
700 1 $aNICIURA, S. C. M.
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