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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/11/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MONTEIRO, J. P.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; ANDRÉ, W. P. P.; RIBEIRO, W. L. C.; MELO, A. C. F. L.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. |
Afiliação: |
JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) -Sobral, CE, Brazil; Gracielle Araújo Frota; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Ana Carolina Fonseca Lindoso Melo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil. |
Título: |
Molecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588. |
Idioma: |
Inglês |
Notas: |
In conjunction with 53rd MSPTM Annual Conference. |
Conteúdo: |
Abstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period. MenosAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a sh... Mostrar Tudo |
Palavras-Chave: |
Beta tubulin; Dosage effects; Resistance to anthelmintics; SNP. |
Thesagro: |
Anti-Helmíntico; Haemonchus contortus; Resistência química. |
Thesaurus Nal: |
Anthelmintics; Drug resistance; Oxfendazole. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166452/1/CNPC-2017-Molecular.pdf
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Marc: |
LEADER 02735nam a2200349 a 4500 001 2079254 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aMONTEIRO, J. P. 245 $aMolecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588.$c4588 500 $aIn conjunction with 53rd MSPTM Annual Conference. 520 $aAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period. 650 $aAnthelmintics 650 $aDrug resistance 650 $aOxfendazole 650 $aAnti-Helmíntico 650 $aHaemonchus contortus 650 $aResistência química 653 $aBeta tubulin 653 $aDosage effects 653 $aResistance to anthelmintics 653 $aSNP 700 1 $aSANTOS, J. M. L. dos 700 1 $aVASCONCELOS, J. F. 700 1 $aFROTA, G. A. 700 1 $aANDRÉ, W. P. P. 700 1 $aRIBEIRO, W. L. C. 700 1 $aMELO, A. C. F. L. 700 1 $aTEIXEIRA, M. 700 1 $aVIEIRA, L. da S. 700 1 $aBEVILAQUA, C. M. L.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
11/02/2020 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OLIVEIRA, P. C. C.; ALVARENGA, C. D.; SANTOS, J. O.; SANTOS, R. F. S.; SILVA, U. T. F.; PARANHOS, B. A. J. |
Afiliação: |
Patrícia C. C. Oliveira; Clarice D. Alvarenga; Jéssica O. Santos; Raila F. S. Santos; Uilca Thamara F. Silva; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA. |
Título: |
Atividade de parasitismo por Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae) em diferentes horários. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 16., 2019, Londrina. Controle biológico: da academia ao campo, rumo à sustentabilidade - anais. Londrina: Sociedade Entomológica do Brasil: Embrapa Soja: Universidade Estadual de Londrina, 2019. |
Páginas: |
p. 191. |
Idioma: |
Português |
Conteúdo: |
O presente trabalho teve como objetivo determinar o horário de maior atividade de parasitismo de fêmeas de Diachasmimorpha longicaudata sobre larvas de Ceratitis capitata. |
Palavras-Chave: |
Fruit fly; Parasitoide. |
Thesagro: |
Ceratitis Capitata; Controle Biológico; Inseto; Mosca das Frutas. |
Thesaurus NAL: |
Diachasmimorpha longicaudata; Insects. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/210648/1/Atividade-de-parasitismo-2019.pdf
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Marc: |
LEADER 01193nam a2200277 a 4500 001 2114297 005 2020-02-11 008 2019 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, P. C. C. 245 $aAtividade de parasitismo por Diachasmimorpha longicaudata (Ashmead) (Hymenoptera$bBraconidae) em diferentes horários.$h[electronic resource] 260 $aIn: SIMPÓSIO DE CONTROLE BIOLÓGICO, 16., 2019, Londrina. Controle biológico: da academia ao campo, rumo à sustentabilidade - anais. Londrina: Sociedade Entomológica do Brasil: Embrapa Soja: Universidade Estadual de Londrina$c2019 300 $ap. 191. 520 $aO presente trabalho teve como objetivo determinar o horário de maior atividade de parasitismo de fêmeas de Diachasmimorpha longicaudata sobre larvas de Ceratitis capitata. 650 $aDiachasmimorpha longicaudata 650 $aInsects 650 $aCeratitis Capitata 650 $aControle Biológico 650 $aInseto 650 $aMosca das Frutas 653 $aFruit fly 653 $aParasitoide 700 1 $aALVARENGA, C. D. 700 1 $aSANTOS, J. O. 700 1 $aSANTOS, R. F. S. 700 1 $aSILVA, U. T. F. 700 1 $aPARANHOS, B. A. J.
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