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| Registros recuperados : 42 | |
| 6. |  | SILVA, C. A. L. da; CAMPANHA, R. B.; MACHADO, J. C.; OLIVEIRA, P. A. de. Análise da composição química de cinco variedades de capim-elefante por métodos Diferentes. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 208-213.| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | AMARAL, H. de O.; SOUZA, O. A. C.; SAMPAIO, B. L.; CAMPANHA, R. B.; MENDONCA, S.; OLIVEIRA, P. A. de. Avaliação de toxidade em Artemia salina de extratos de biomassas e resíduos agrícolas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 63.| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | ALVES, V. A.; MORAES, A. S. da S.; MENDONCA, S.; OLIVEIRA, P. A. de; SOARES, I. P. Avaliação da estabilidade oxidativa do biodiesel na presença de extratos. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 22.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | CAMPANHA, R. B.; ROCHA, J. L. da C.; MORAES, A. S. da S.; OLIVEIRA, P. A. de; MENDONCA, S. Atividade antioxidante de extratos hidroalcoólicos obtidos de diferentes biomassas lignocelulósicas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 65-72 il.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | DOMINGUES; FREITAS, C de S.; NASCIMENTO, T. P. do; CAMPANHA, R. B.; OLIVEIRA, P. A. de; MENDONCA, S. Efeito das condições de secagem sobre a umidade de espécies de macroalgas da costa brasileira. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 7., 2023, Brasília, DF. Anais... Brasília, DF: Embrapa, 2023. p. 14.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | MIDORIKAWA, G. E. O.; SANTOS, L. C. Z.; OLIVEIRA, P. A. de; FAVARO, L. C. de L. Prospecção, seleção e identificação de bactérias produtoras de corantes. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 195-200 il.| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | SOUZA, O. C. A.; MORAES, A.; SAMPAIO, B. L.; CAMPANHA, R. B.; MENDONCA, S.; OLIVEIRA, P. A. de. Quantification of total phenolics and flavonoids content in agricultural residues. In: INTERNATIONAL CONGRESS ON BIOACTIVE COMPOUNDS, 1.; INTERNATIONAL WORKSHOP ON BIOACTIVE COMPOUNDS, 2., 2018, Campinas, SP. Food design and health nutrition. Campinas: UNICAMP, 2018. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | MORAES, A. S. da S.; SAMPAIO, B. L.; SOUZA, O. C. A.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; BARRETO, L. C. L. de S.; MENDONCA, S. Atividade antioxidante de extrato fluido a partir de resíduos de oleaginosas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 20.| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | FALCAO, R.; FRANCO, P. F.; CONCEIÇÃO, A. A.; MIDORIKAWA, G. E. O.; SIQUEIRA, F. G. de; OLIVEIRA, P. A. de; FAVARO, L. C. de L. Percepção das cores com ImageJ: elaboração de um guia para análise de imagens de microrganismos produtores de corantes naturais. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 201-208 il.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 42 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
07/07/2022 |
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Data da última atualização: |
08/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C. |
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Afiliação: |
FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNB; FRANCISCO DE ASSIS DOS SANTOS DINIZ, UNB; CAMILA DE MORAES RÊGO-MACHADO, UNB; NATÁLIA CHAGAS FREITAS; FABRÍCIO BARBOSA MONTEIRO ARRAES; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; LEILA LOURENÇO FURTADO, UNB; KAREN OFUJI OSIRO, UNB; NATÁLIA LIMA DE SOUSA, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; THIAGO BÉRGAMO CARDOSO, SEMPRE AgTech; LILIANE MARCIA MERTZ HENNING, CNPSO; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; SÉRGIO ENRIQUE FEINGOLD, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; WAYNE B. HUNTER, USDA-ARS, United States; MARIA FATIMA GROSSI DE SA, Cenargen; ADILSON KENJI KOBAYASHI, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; THAÍS RIBEIRO SANTIAGO, UNB; HUGO BRUNO CORREA MOLINARI, SEMPRE AgTech. |
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Título: |
CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728. |
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DOI: |
https://doi.org/10.3389/fbioe.2022.913728 |
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Idioma: |
Inglês |
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Conteúdo: |
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe. MenosClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public pe... Mostrar Tudo |
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Palavras-Chave: |
Exogenous dsRNA; Genome editin; Genome editing; Offtargets; Public acceptance; Regulatory aspects. |
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Thesagro: |
Genoma. |
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Thesaurus NAL: |
Gene silencing; Nanotechnology; Toxicity. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
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520 $aClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.
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773 $tFrontiers in Bioengineering and Biotechnology$gv. 10, 2022. Article 913728.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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