Data/hora: 15/06/2024 05:41:36

Biblioteca(s): | Embrapa Agricultura Digital; Embrapa Agroenergia.
Data corrente: | 12/09/2017
Data da última atualização: | 15/01/2018
Autoria: | BASSO, M. F.; DIAS, B. B. A.; RIBEIRO, A. P.; MARTINS, P. K.; SOUZA, W. R.; OLIVEIRA, N. G. DE.; NAKAYAMA, T. J.; CASARI, R. A das C. N.; SANTIAGO, T. R.; VINECKY, F.; JUNGMANN, L.; SOUSA, C. A. F. de; OLIVEIRA, P. A. de; SOUZA, S. A. C. D. de; CANCADO, G. M. de A.; KOBAYASHI, A. K.; MOLINARI, H. B. C.
Afiliação: | MARCOS FERNANDO BASSO; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ANA PAULA RIBEIRO; POLYANA KELLY MARTINS; WAGNER RODRIGO DE SOUZA; NELSON GERALDO DE OLIVEIRA; THIAGO JONAS NAKAYAMA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; THAIS RIBEIRO SANTIAGO; FELIPE VINECKY; LETICIA JUNGMANN CANCADO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; SILVANA APARECIDA CRESTE DIAS DE SOUZA; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.
Título: | Improved genetic transformation of sugarcane (Saccharum spp.) embryogenic callus mediated by Agrobacterium tumefaciens.
Ano de publicação: | 2017
Fonte/Imprenta: | Current Protocols in Plant Biology, v. 2, n. 3, p. 221-239, Sept. 2017.
Idioma: | Inglês

Conteúdo:

Sugarcane (Saccharum spp.) is a monocotyledonous semi-perennial C4 grass of the Poaceae family. Its capacity to accumulate high content of sucrose and biomass makes it one of the most important crops for sugar and biofuel production. Conventional methods of sugarcane breeding have shown several limitations due to its complex polyploid and aneuploid genome. However, improvement by biotechnological engineering is currently the most promising alternative to introduce economically important traits. In this work, we present an improved protocol for Agrobacterium tumefaciens-mediated transformation of commercial sugarcane hybrids using immature top stalk-derived embryogenic callus cultures. The callus cultures are transformed with preconditioned A. tumefaciens carrying a binary vector that encodes expression cassettes for
a gene of interest and the bialaphos resistance gene (bar confers resistance to glufosinate-ammonium herbicide). This protocol has been used to successfully transform a commercial sugarcane cultivar, SP80-3280, highlighting: (i) reduced recalcitrance and oxidation; (ii) high yield of embryogenic callus; (iii) improved selection; and (iv) shoot regeneration and rooting of the transformed plants. Altogether, these improvements generated a transformation efficiency of 2.2%. This protocol provides a reliable tool for a routine procedure for sugarcane improvement by genetic engineering.

Palavras-Chave: | Agrotransformation; Cultivar SP80-3280; Immature top stalks; Transgenic bioenergy crops.
Thesagro: | Agrobacterium Tumefaciens; Biomassa; Cana de açúcar; Engenharia genética.
Thesaurus Nal: | Agrobacterium radiobacter; Genetic engineering; Saccharum; Sugarcane.

Categoria do assunto:

--
X Pesquisa, Tecnologia e Engenharia

Registro original: | Embrapa Agroenergia (CNPAE)

Exemplares:

CNPTIA - (19274-1) - UPC - AP - DD -  -  -  - DIS
CNPAE - (3167-1) - UPC - AP - DD -  -  -  - DIS