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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/08/2021 |
Data da última atualização: |
12/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MONTEIRO, C.; COELHO, L.; PAULA, L. G. F. de; FERNANDES, E. K. K.; DOLINSKIC, C.; BITTENCOURT, V. R. E. P.; FURLONGE, J.; PRATA, M. C. de A. |
Afiliação: |
CAIO MONTEIRO, Universidade Federal de Goiás; LETÍCIA COELHO, Universidade Federal de Goiás; LUIZA GABRIELLA FERREIRA DE PAULA, Universidade Federal de Goiás; ÉVERTON KORT KAMP FERNANDES, Universidade Federal de Goiás; CLAUDIA DOLINSKIC, Universidade Estadual Norte Fluminense Darcy Ribeiro; VÂNIA RITA ELIAS PINHEIRO BITTENCOURT, Universidade Federal Rural do Rio de Janeiro; JOHN FURLONGE; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL. |
Título: |
Efficacy of entomopathogenic nematodes in insect cadaver formulation against engorged females of Rhipicephalus microplus (Acari: Ixodidae) in semi-field conditions. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 11, 101313, 2020. |
DOI: |
https://doi.org/10.1016/j.ttbdis.2019.101313 |
Idioma: |
Inglês |
Conteúdo: |
The present study evaluated, in the laboratory, the virulence of Heterorhabditis bacteriophora LPP30 against engorged females of Rhipicephalus microplus; in addition, we evaluated, in semi-field tests, the effects of four isolates of Heterorhabditis spp. (i.e., H. bacteriophora HP88, Heterorhabditis baujardi LPP7, Heterorhabditis indica LPP1 and H. bacteriophora LPP30) in insect cadaver formulation against the non-parasitic phase of R. microplus. In the first experiment (in vitro), engorged females were exposed, in Petri dish, to H. bacteriophora LPP30 at 75, 150, 300, 600, 1200 and 2400 nematodes/tick (10 ticks per concentration tested). In the second experiment (semi-field), five engorged females and four cadavers of Galleria mellonella infected with nematodes were placed in pots with soil and grown Brachiaria decumbens; the pots were hold outdoor, exposed to natural environment conditions during the tests. In the third experiment (semi-field), 65 days after the cadavers had been placed in the pots for the second experiment, new engorged females (five per pot) were placed in the pots of the groups treated with H. bacteriophora HP88 or H. baujardi LPP7, to assess their persistence in the soil. In the first test, the percent control was higher than 95% in all treatment groups. In the second experiment, at day 22, the mean mortality rate was 78% in the groups treated with H. bacteriophora LPP30 or H. indica LPP1, and reached 100% and 98% when treated with H. bacteriophora HP88 and H. baujardi LPP7, respectively. In this experiment, the egg-laying inhibition index was higher than 90% in the groups treated with H. bacteriophora HP88 (97.2%) or H. baujardi LPP7 (91.9%). In the third experiment with H. bacteriophora HP88 and H. baujardi LPP7, the egg-laying inhibition index was 59.1% and 43.1%, respectively. We concluded that the isolate LPP30 was highly virulent under laboratory conditions, whereas in semi-field tests, HP88 and LPP7 were the most effective isolates. Moreover, HP88 and LPP7 remained active against engorged females of R. microplus in the soil for 65 days after application of EPN-infected cadavers of G. mellonella. MenosThe present study evaluated, in the laboratory, the virulence of Heterorhabditis bacteriophora LPP30 against engorged females of Rhipicephalus microplus; in addition, we evaluated, in semi-field tests, the effects of four isolates of Heterorhabditis spp. (i.e., H. bacteriophora HP88, Heterorhabditis baujardi LPP7, Heterorhabditis indica LPP1 and H. bacteriophora LPP30) in insect cadaver formulation against the non-parasitic phase of R. microplus. In the first experiment (in vitro), engorged females were exposed, in Petri dish, to H. bacteriophora LPP30 at 75, 150, 300, 600, 1200 and 2400 nematodes/tick (10 ticks per concentration tested). In the second experiment (semi-field), five engorged females and four cadavers of Galleria mellonella infected with nematodes were placed in pots with soil and grown Brachiaria decumbens; the pots were hold outdoor, exposed to natural environment conditions during the tests. In the third experiment (semi-field), 65 days after the cadavers had been placed in the pots for the second experiment, new engorged females (five per pot) were placed in the pots of the groups treated with H. bacteriophora HP88 or H. baujardi LPP7, to assess their persistence in the soil. In the first test, the percent control was higher than 95% in all treatment groups. In the second experiment, at day 22, the mean mortality rate was 78% in the groups treated with H. bacteriophora LPP30 or H. indica LPP1, and reached 100% and 98% when treated with H. bacteriophora HP8... Mostrar Tudo |
Palavras-Chave: |
Heterorhabditis baujardi. |
Thesagro: |
Bovino; Carrapato; Controle Biológico. |
Thesaurus Nal: |
Galleria mellonella; Heterorhabditis bacteriophora; Heterorhabditis indica. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03169naa a2200301 a 4500 001 2133485 005 2021-08-12 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ttbdis.2019.101313$2DOI 100 1 $aMONTEIRO, C. 245 $aEfficacy of entomopathogenic nematodes in insect cadaver formulation against engorged females of Rhipicephalus microplus (Acari$bIxodidae) in semi-field conditions.$h[electronic resource] 260 $c2020 520 $aThe present study evaluated, in the laboratory, the virulence of Heterorhabditis bacteriophora LPP30 against engorged females of Rhipicephalus microplus; in addition, we evaluated, in semi-field tests, the effects of four isolates of Heterorhabditis spp. (i.e., H. bacteriophora HP88, Heterorhabditis baujardi LPP7, Heterorhabditis indica LPP1 and H. bacteriophora LPP30) in insect cadaver formulation against the non-parasitic phase of R. microplus. In the first experiment (in vitro), engorged females were exposed, in Petri dish, to H. bacteriophora LPP30 at 75, 150, 300, 600, 1200 and 2400 nematodes/tick (10 ticks per concentration tested). In the second experiment (semi-field), five engorged females and four cadavers of Galleria mellonella infected with nematodes were placed in pots with soil and grown Brachiaria decumbens; the pots were hold outdoor, exposed to natural environment conditions during the tests. In the third experiment (semi-field), 65 days after the cadavers had been placed in the pots for the second experiment, new engorged females (five per pot) were placed in the pots of the groups treated with H. bacteriophora HP88 or H. baujardi LPP7, to assess their persistence in the soil. In the first test, the percent control was higher than 95% in all treatment groups. In the second experiment, at day 22, the mean mortality rate was 78% in the groups treated with H. bacteriophora LPP30 or H. indica LPP1, and reached 100% and 98% when treated with H. bacteriophora HP88 and H. baujardi LPP7, respectively. In this experiment, the egg-laying inhibition index was higher than 90% in the groups treated with H. bacteriophora HP88 (97.2%) or H. baujardi LPP7 (91.9%). In the third experiment with H. bacteriophora HP88 and H. baujardi LPP7, the egg-laying inhibition index was 59.1% and 43.1%, respectively. We concluded that the isolate LPP30 was highly virulent under laboratory conditions, whereas in semi-field tests, HP88 and LPP7 were the most effective isolates. Moreover, HP88 and LPP7 remained active against engorged females of R. microplus in the soil for 65 days after application of EPN-infected cadavers of G. mellonella. 650 $aGalleria mellonella 650 $aHeterorhabditis bacteriophora 650 $aHeterorhabditis indica 650 $aBovino 650 $aCarrapato 650 $aControle Biológico 653 $aHeterorhabditis baujardi 700 1 $aCOELHO, L. 700 1 $aPAULA, L. G. F. de 700 1 $aFERNANDES, E. K. K. 700 1 $aDOLINSKIC, C. 700 1 $aBITTENCOURT, V. R. E. P. 700 1 $aFURLONGE, J. 700 1 $aPRATA, M. C. de A. 773 $tTicks and Tick-borne Diseases$gv. 11, 101313, 2020.
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
03/12/1997 |
Data da última atualização: |
03/12/1997 |
Autoria: |
BODDEY, R. M.; OLIVEIRA, O. C. de; ALVES, B. J. R.; URQUIAGA, S. |
Título: |
Field application of the 15N isotope dilution technique for the reliable quantification of plant-associated biological nitrogen fixation. |
Ano de publicação: |
1995 |
Fonte/Imprenta: |
Fertilizer Research, Netherlands, v.42, p.77-87, 1995. |
Idioma: |
Inglês |
Conteúdo: |
To apply the isotope dilution (ID) technique, it is necessary to grow the "N2-fixing" crop in a soil where the mineral N is labelled with 15N. Normally the "N2-fixing" crop and a suitable non-N2-fixing control crop are grown in the same labelled soil and the 15N enrichment of the control crop is assumed to be equal to the 15N enrichment of the nitrogen (N) derived from the soil in the "N2-fixing" crop. In this case the proportion of unlabelled N being derived from the air via biological N2 fixation (BNF) in the "N2-fixing" crop will be proportional to the dilution of the enrichment of the N derived from the labelled soil. To label the soil, the technique most often used is to add a single addition of 15N-labelled N fertilizer shortly before, at, or shortly after, the planting of the crops. Data in the literature clearly show that this technique results in a rapid fall in the 15N enrichment of soil mineral N with time. Under these conditions, if the control and the "N2-fixing" crops have different patterns of N uptake from the soil they will inevitably obtain different 15N enrichments in the soil-derived N. In this case the isotope dilution technique cannot be applied, or if it is, there will be an error introduced into, the estimate of the contribution of N derived from BNF. Several experiments are described which explore different strategies of application of the ID technique to attempt to attenuate the errors involved. The results suggest that it is wise to use slow-release forms of labelled N, or in some cases, multiple additions, to diminish temporal changes in the 15N enrichment of soil mineral N. The use of several control crops producers a range of different estimates of the BNF contributions to the "N2-fixing" crops, and the extent of this range gives a measure of the accuracy of the estimates. Likewise the use of more than one 15N enrichment technique in the same experiment will also give a range of estimates which can be treated similarly. The potential of other techniques, such as sequential harvesting of both control and test crops, are also discussed. MenosTo apply the isotope dilution (ID) technique, it is necessary to grow the "N2-fixing" crop in a soil where the mineral N is labelled with 15N. Normally the "N2-fixing" crop and a suitable non-N2-fixing control crop are grown in the same labelled soil and the 15N enrichment of the control crop is assumed to be equal to the 15N enrichment of the nitrogen (N) derived from the soil in the "N2-fixing" crop. In this case the proportion of unlabelled N being derived from the air via biological N2 fixation (BNF) in the "N2-fixing" crop will be proportional to the dilution of the enrichment of the N derived from the labelled soil. To label the soil, the technique most often used is to add a single addition of 15N-labelled N fertilizer shortly before, at, or shortly after, the planting of the crops. Data in the literature clearly show that this technique results in a rapid fall in the 15N enrichment of soil mineral N with time. Under these conditions, if the control and the "N2-fixing" crops have different patterns of N uptake from the soil they will inevitably obtain different 15N enrichments in the soil-derived N. In this case the isotope dilution technique cannot be applied, or if it is, there will be an error introduced into, the estimate of the contribution of N derived from BNF. Several experiments are described which explore different strategies of application of the ID technique to attempt to attenuate the errors involved. The results suggest that it is wise to use slow-releas... Mostrar Tudo |
Palavras-Chave: |
BNF; FBN; Fixacao biologica de nitrogenio. |
Thesagro: |
Diluição Isotópica. |
Thesaurus NAL: |
nitrogen fixation; tracer techniques. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02805naa a2200229 a 4500 001 1620062 005 1997-12-03 008 1995 bl --- 0-- u #d 100 1 $aBODDEY, R. M. 245 $aField application of the 15N isotope dilution technique for the reliable quantification of plant-associated biological nitrogen fixation. 260 $c1995 520 $aTo apply the isotope dilution (ID) technique, it is necessary to grow the "N2-fixing" crop in a soil where the mineral N is labelled with 15N. Normally the "N2-fixing" crop and a suitable non-N2-fixing control crop are grown in the same labelled soil and the 15N enrichment of the control crop is assumed to be equal to the 15N enrichment of the nitrogen (N) derived from the soil in the "N2-fixing" crop. In this case the proportion of unlabelled N being derived from the air via biological N2 fixation (BNF) in the "N2-fixing" crop will be proportional to the dilution of the enrichment of the N derived from the labelled soil. To label the soil, the technique most often used is to add a single addition of 15N-labelled N fertilizer shortly before, at, or shortly after, the planting of the crops. Data in the literature clearly show that this technique results in a rapid fall in the 15N enrichment of soil mineral N with time. Under these conditions, if the control and the "N2-fixing" crops have different patterns of N uptake from the soil they will inevitably obtain different 15N enrichments in the soil-derived N. In this case the isotope dilution technique cannot be applied, or if it is, there will be an error introduced into, the estimate of the contribution of N derived from BNF. Several experiments are described which explore different strategies of application of the ID technique to attempt to attenuate the errors involved. The results suggest that it is wise to use slow-release forms of labelled N, or in some cases, multiple additions, to diminish temporal changes in the 15N enrichment of soil mineral N. The use of several control crops producers a range of different estimates of the BNF contributions to the "N2-fixing" crops, and the extent of this range gives a measure of the accuracy of the estimates. Likewise the use of more than one 15N enrichment technique in the same experiment will also give a range of estimates which can be treated similarly. The potential of other techniques, such as sequential harvesting of both control and test crops, are also discussed. 650 $anitrogen fixation 650 $atracer techniques 650 $aDiluição Isotópica 653 $aBNF 653 $aFBN 653 $aFixacao biologica de nitrogenio 700 1 $aOLIVEIRA, O. C. de 700 1 $aALVES, B. J. R. 700 1 $aURQUIAGA, S. 773 $tFertilizer Research, Netherlands$gv.42, p.77-87, 1995.
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