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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/07/2019 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, S. E. M.; GIMENES, M. A.; OLIVEIRA JUNIOR, J. O. L. de; COSTA, M. F.; GOMES, M. F. C.; VALENTE, S. E. S. |
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Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; JOSE OSCAR LUSTOSA DE O JUNIOR, CPAMN; M. F. COSTA, UFPI; M. F. C.GOMES, UESPI; S. E. S.VALENTE, UFPI. |
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Título: |
Consequences of accelerated aging for DNA integrity and seed germination of cowpea. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, article gmr18304, 2019. |
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DOI: |
10.4238/gmr18304 |
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Idioma: |
Inglês |
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Conteúdo: |
Deterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. MenosDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processe... Mostrar Tudo |
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Palavras-Chave: |
Orthodox seeds; Seed bank. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus Nal: |
Genetic resources. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199456/1/gmr18304-consequences-accelerated-aging-dna-integrity-and-seed-germination-cowpea.pdf
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Marc: |
LEADER 02315naa a2200241 a 4500
001 2110566
005 2020-03-05
008 2019 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr18304$2DOI
100 1 $aSANTOS, S. E. M.
245 $aConsequences of accelerated aging for DNA integrity and seed germination of cowpea.$h[electronic resource]
260 $c2019
520 $aDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars.
650 $aGenetic resources
650 $aVigna Unguiculata
653 $aOrthodox seeds
653 $aSeed bank
700 1 $aGIMENES, M. A.
700 1 $aOLIVEIRA JUNIOR, J. O. L. de
700 1 $aCOSTA, M. F.
700 1 $aGOMES, M. F. C.
700 1 $aVALENTE, S. E. S.
773 $tGenetics and Molecular Research$gv. 18, n. 3, article gmr18304, 2019.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Roraima. |
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Data corrente: |
28/02/2012 |
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Data da última atualização: |
31/10/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAÚJO, M. da C. da R.; OLIVEIRA, N. T. de; FLORES, P. S.; CHAGAS, E. A.; CASTRO, A. M. de; VENÂNCIO, J. B.; COUCEIRO, M. A.; PASQUAL, M. |
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Afiliação: |
MARIA DA CONCEIÇÃO DA ROCHA ARAÚJO, UFRR; NATALIA TRAJANO DE OLIVEIRA, UFRR; PATRICIA SILVA FLORES, CPAF-AC; EDVAN ALVES CHAGAS, CPAF-RR; ALBERTO MOURA DE CASTRO, UFRR; JEFFERSON BITTENCOURT VENÂNCIO, UFRR; MARCIO AKIRA COUCEIRO, UFRR; MOACIR PASQUAL, UFLA. |
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Título: |
Influência do ácido naftaleno acético e luminosidade no alongamento in vitro de orquídea. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Inovar com sustentabilidade: palestras, posters, minicursos. Itajaí: ABCTP: SBFP, 2011. |
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Páginas: |
2 p. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Objetivou-se avaliar o uso de diferentes concentrações de ácido naftaleno-acético (ANA) e intensidades luminosas no alongamento in vitro de plântulas de híbridas de orquídeas oriundas do cruzamento entre Cattleya walkeriana 12 x Cattleya walkeriana "Lobo mau". |
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Palavras-Chave: |
Ácido naftaleno-acético (ANA); Brotar; Cattleya walkeriana; Micropropagación; Multiplicação in vitro; Orchid; Propagación vegetativa; Sustancias de crecimiento vegetal. |
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Thesagro: |
Ácido Naftalenoacético; Auxina; Brotacao Induzida; Micropropagação; Orquídea; Propagação Vegetativa; Regulador de Crescimento. |
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Thesaurus NAL: |
Auxins; Micropropagation; Naphthaleneacetic acid; Plant growth substances; Sprouting; Vegetative propagation. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184700/1/24145.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/55356/1/188-Nilma-ANA.pdf
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Marc: |
LEADER 01792nam a2200457 a 4500
001 1916866
005 2023-10-31
008 2011 bl uuuu u00u1 u #d
100 1 $aARAÚJO, M. da C. da R.
245 $aInfluência do ácido naftaleno acético e luminosidade no alongamento in vitro de orquídea.
260 $aIn: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Inovar com sustentabilidade: palestras, posters, minicursos. Itajaí: ABCTP: SBFP$c2011
300 $a2 p.$c1 CD-ROM.
520 $aObjetivou-se avaliar o uso de diferentes concentrações de ácido naftaleno-acético (ANA) e intensidades luminosas no alongamento in vitro de plântulas de híbridas de orquídeas oriundas do cruzamento entre Cattleya walkeriana 12 x Cattleya walkeriana "Lobo mau".
650 $aAuxins
650 $aMicropropagation
650 $aNaphthaleneacetic acid
650 $aPlant growth substances
650 $aSprouting
650 $aVegetative propagation
650 $aÁcido Naftalenoacético
650 $aAuxina
650 $aBrotacao Induzida
650 $aMicropropagação
650 $aOrquídea
650 $aPropagação Vegetativa
650 $aRegulador de Crescimento
653 $aÁcido naftaleno-acético (ANA)
653 $aBrotar
653 $aCattleya walkeriana
653 $aMicropropagación
653 $aMultiplicação in vitro
653 $aOrchid
653 $aPropagación vegetativa
653 $aSustancias de crecimiento vegetal
700 1 $aOLIVEIRA, N. T. de
700 1 $aFLORES, P. S.
700 1 $aCHAGAS, E. A.
700 1 $aCASTRO, A. M. de
700 1 $aVENÂNCIO, J. B.
700 1 $aCOUCEIRO, M. A.
700 1 $aPASQUAL, M.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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