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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
ELEOTÉRIO, R. B.; SEPÚLVEDA, R. V.; REIS, E. C. C.; VALENTE, F. L.; BORGES, A P. B. |
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Afiliação: |
RENATO B. ELEOTÉRIO, UFV; RODRIGO V. SEPULVEDA, UFV; EMILY C. C. REIS, UFV; FABRÍCIO L. VALENTE, UFV; ANDRÉA P. B. BORGES, UFV. |
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Título: |
Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 423-430, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. MenosTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media... Mostrar Tudo |
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Palavras-Chave: |
Bone marrow stem cells; Cell therapy; Células tronco mesenquimais; Engenharia de tecido; Medicina regenerativa; Mesenchymal stromal cells; Regenerative medicine; Terapia celular. |
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Thesagro: |
Coelho; Medula osséa. |
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Thesaurus Nal: |
Rabbits; Tissue engineering. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143602/1/Isolation-expansion.pdf
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Marc: |
LEADER 02539naa a2200313 a 4500
001 2045981
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aELEOTÉRIO, R. B.
245 $aIsolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'.
260 $c2016
520 $aTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation.
650 $aRabbits
650 $aTissue engineering
650 $aCoelho
650 $aMedula osséa
653 $aBone marrow stem cells
653 $aCell therapy
653 $aCélulas tronco mesenquimais
653 $aEngenharia de tecido
653 $aMedicina regenerativa
653 $aMesenchymal stromal cells
653 $aRegenerative medicine
653 $aTerapia celular
700 1 $aSEPÚLVEDA, R. V.
700 1 $aREIS, E. C. C.
700 1 $aVALENTE, F. L.
700 1 $aBORGES, A P. B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 423-430, maio. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/12/2013 |
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Data da última atualização: |
02/01/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MOURAO, C. S.; MENEZES, C. B. de; TARDIN, F. D.; SILVA, A. R. da; BERNARDINO, K. da C.; SILVA, K. J. da; SANTOS, C. V. dos; OLIVEIRA, M. S.; MOURA, S. M.; ABREU, A. L. de; MACHADO, P. H. B.; SCHAFFERT, R. E. |
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Afiliação: |
CAMILA SIMAO MOURAO, Discente de graduação na UNIFEMM, Sete Lagoas, MG.; CICERO BESERRA DE MENEZES, CNPMS; FLAVIO DESSAUNE TARDIN, CNPMS; ADELMO RESENDE DA SILVA, CNPMS; KARINE DA COSTA BERNARDINO, Mestranda em Melhoramento Genético, UFV, Viçosa.; KARLA JORGE DA SILVA, Discentes de graduação da UFSJ, Campus Sete Lagoas, MG.; CRISLENE VIEIRA DOS SANTOS, Discentes de graduação da UFSJ, Campus de Sete Lagoas , MG.; MATEUS SATURNINO OLIVEIRA, Discentes de graduação da UFSJ, Campus de Sete Lagoas, MG.; SAMUEL MOREIRA MOURA, Discentes de graduação da UFSJ, Campus de Sete Lagoas, MG.; ALICE LAGOEIRO DE ABREU, Discentes de graduação da UFSJ, Campus de Sete Lagoas , MG.; PEDRO HENRIQUE BORGES MACHADO, Discente de graduação na UNIFEMM, Sete Lagoas, MG.; ROBERT EUGENE SCHAFFERT, CNPMS. |
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Título: |
Adaptabilidade e estabilidade de híbridos de sorgo granífero em condições de safrinha. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 7., 2013, Uberlândia. Variedade melhorada: a força da nossa agricultura: anais. Viçosa, MG: SBMP, 2013. |
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Páginas: |
p. 1014-1017. |
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Idioma: |
Português |
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Thesagro: |
Melhoramento genético vegetal; Sorghum bicolor. |
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Thesaurus NAL: |
Hybrids; Plant breeding. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94592/1/Adaptabilidade-estabilidade-37.pdf
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Marc: |
LEADER 00980nam a2200289 a 4500
001 1974356
005 2014-01-02
008 2013 bl uuuu u00u1 u #d
100 1 $aMOURAO, C. S.
245 $aAdaptabilidade e estabilidade de híbridos de sorgo granífero em condições de safrinha.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 7., 2013, Uberlândia. Variedade melhorada: a força da nossa agricultura: anais. Viçosa, MG: SBMP$c2013
300 $ap. 1014-1017.
650 $aHybrids
650 $aPlant breeding
650 $aMelhoramento genético vegetal
650 $aSorghum bicolor
700 1 $aMENEZES, C. B. de
700 1 $aTARDIN, F. D.
700 1 $aSILVA, A. R. da
700 1 $aBERNARDINO, K. da C.
700 1 $aSILVA, K. J. da
700 1 $aSANTOS, C. V. dos
700 1 $aOLIVEIRA, M. S.
700 1 $aMOURA, S. M.
700 1 $aABREU, A. L. de
700 1 $aMACHADO, P. H. B.
700 1 $aSCHAFFERT, R. E.
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Embrapa Milho e Sorgo (CNPMS) |
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