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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
06/11/2002 |
Data da última atualização: |
06/11/2002 |
Autoria: |
ZHANG, J.; MASSMANN, G. A.; MIRABILE, C. P.; FIGUEROA, J. P. |
Título: |
Nonpregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Biology of Reproduction, v. 60, n. 5, p. 1198-1203, 1999. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the uterus serves more than one physiological role. In myometrium it mar be a uterorelaxant and regulate glucose utilization, and in endometrium and myometrium it mar regulate blood flow. MenosThe aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the ute... Mostrar Tudo |
Thesagro: |
Endocrinologia; Estrógeno; Ovino; Prenhez; Reprodução Animal; Útero. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02338naa a2200229 a 4500 001 1528831 005 2002-11-06 008 1999 bl uuuu u00u1 u #d 100 1 $aZHANG, J. 245 $aNonpregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. 260 $c1999 520 $aThe aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the uterus serves more than one physiological role. In myometrium it mar be a uterorelaxant and regulate glucose utilization, and in endometrium and myometrium it mar regulate blood flow. 650 $aEndocrinologia 650 $aEstrógeno 650 $aOvino 650 $aPrenhez 650 $aReprodução Animal 650 $aÚtero 700 1 $aMASSMANN, G. A. 700 1 $aMIRABILE, C. P. 700 1 $aFIGUEROA, J. P. 773 $tBiology of Reproduction$gv. 60, n. 5, p. 1198-1203, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Algodão. |
Data corrente: |
16/02/2024 |
Data da última atualização: |
16/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
SILVA, A. de J.; IKEDA, F. S.; CAVALIERI, S. D.; OLIVEIRA, D. S.; WOIAND, H. M. G.; SILVA, C. C.; OLIVEIRA, L. R. de. |
Afiliação: |
ALEIXA DE JESUS SILVA, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; FERNANDA SATIE IKEDA, CPAMT; SIDNEI DOUGLAS CAVALIERI, CNPA; DELIS S. OLIVEIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO; HELEN MAILA GABE WOIAND, EPR CONSULTORIA & PESQUISA AGRONÔMICA; CRISTIANA C. SILVA, BAYER; LUCAS R. DE OLIVEIRA, LAVORO. |
Título: |
Dessecação acelerada de capim-marandu com a aplicação sequencial de glyphosate e amônio-glufosinate. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Weed Control Journal, v. 22, e202300747, 2023. |
ISSN: |
2763-8332 |
Idioma: |
Português |
Thesagro: |
Palhada. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162053/1/2023-cpamt-fsi-dessecacao-acelerada-campim-marandu-aplicacao-sequencial-glyphosate-amonio-glufosinate.pdf
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Marc: |
LEADER 00624naa a2200205 a 4500 001 2162053 005 2024-02-16 008 2023 bl uuuu u00u1 u #d 022 $a2763-8332 100 1 $aSILVA, A. de J. 245 $aDessecação acelerada de capim-marandu com a aplicação sequencial de glyphosate e amônio-glufosinate.$h[electronic resource] 260 $c2023 650 $aPalhada 700 1 $aIKEDA, F. S. 700 1 $aCAVALIERI, S. D. 700 1 $aOLIVEIRA, D. S. 700 1 $aWOIAND, H. M. G. 700 1 $aSILVA, C. C. 700 1 $aOLIVEIRA, L. R. de 773 $tWeed Control Journal$gv. 22, e202300747, 2023.
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