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| Registros recuperados : 21 | |
| 7. |  | KOHLER, T. W.; KUNDE, R. J.; KELLING, P. M.; REIS, D. A.; MARTINAZZO, R. Estoques de carbono e de nitrogênio em cambissolo háplico sob plantio de acácia negra. SIMPÓSIO INTERNACIONAL DE QUALIDADE AMBIENTAL, 10., 2015, Porto Alegre, RS. Regulamentação Ambiental, Desenvolvimentoe Inovação. Anais...Porto Alegre: PUCRS, 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | RIBEIRO, P. L.; BAMBERG, A. L.; OLIVEIRA, A. C. B. de; REIS, D. A.; MARTINAZZO, R. Atributos físicos de um planossolo cultivado com soja sob plantio direto e preparo convencional, In: REUNIÃO DE PESQUISA DA SOJA DA REGIÃO SUL, 40., Pelotas, 2014. Atas e Resumos. Pelotas: Embrapa Clima Temperado, 2014. 473 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | FAGUNDES, M. O.; REIS, D. A.; PORTELLA, R. B.; PERINA, F. J.; BOGIANI, J. C. Qualidade de um latossolo sob plantio convencional e sistema plantio direto no cerrado baiano, Brasil. Revista Ibero Americana de Ciências Ambientais, v. 10, n. 1, p. 281-297, 2019.| Biblioteca(s): Embrapa Territorial. |
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| 13. | | RIBEIRO, P. L.; REIS, D. A.; MARTINAZZO, R.; GRECCO, M. F.; OLIVEIRA, A. C. B. de; BAMBERG, A. L. Armazenamento de água em planossolo cultivado com soja sob plantio direto e preparo convencional. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | RIBEIRO, P. L.; BAMBERG, A. L.; REIS, D. A.; OLIVEIRA, A. C. B. de; OLIVEIRA, A. C. B. de. Condições físico-hídricas de Planossolo cultivado com soja em plantio direto e preparo convencional. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 9, p. 1484-1491, set. 2016. Título em inglês: Physico-hydric conditions of an Alfisol cultivated with soybean under no-tillage and conventional tillage.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 15. | | KELLING, P. M.; REIS, D. A.; RIBEIRO, P. L.; MARTINAZZO, R.; BAMBERG, A. L.; VALGAS, R. A. Estoques de carbono em solo sob plantio de acácia no Rio Grande do Sul. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel, 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | RIBEIRO, P. L.; REIS, D. A.; KELLING, P. M.; BAMBERG, A. L.; OLIVEIRA, A. C. B. de; MARTINAZZO, R. Atributos físico-hídricos de um planossolo cultivado com soja sob plantio direto e preparo convencional do solo. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel, 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | RIBEIRO, P. L.; REIS, D. A.; MARTINAZZO, R.; GRECCO, M. F.; OLIVEIRA, A. C. B. de; BAMBERG, A. L. Produção de soja em planossolo. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?. Resumos e palestras... Pelotas: Embrapa Clima Temperado, 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | FEITOSA JÚNIOR, F. R.; REIS, D. A.; PORTELLA, R. B.; SANTOS, J. R. S.; PERINA, F. J.; BOGIANI, J. C. Sistemas de manejo e parâmetros da matéria orgânica de um solo do cerrado baiano, Brasil. Revista Ibero Americana de Ciências Ambientais, v. 10, n. 3, p. 298-312, 2019.| Biblioteca(s): Embrapa Territorial. |
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| 20. | | RIBEIRO, P. L.; BAMBERG, A. L.; REIS, D. A.; MARTINAZZO, R.; GRECCO, M. F.; OLIVEIRA, A. C. B. de. Variação do conteúdo de água armazenada de um planossolo cultivado com soja sob plantio direto e preparo convencional. In: REUNIÃO SUL-BRASILEIRA DE CIÊNCIA DO SOLO, 10., 2014, Pelotas. Fatos e mitos em ciência do solo. Pelotas: Sociedade Brasileira de Ciência do Solo; UFPEL, 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 21 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
02/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; ELISETE PAINS RODRIGUES, UEL; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; ANDRÉ LUIZ MARTINEZ OLIVEIRA, UEL; MARIANGELA HUNGRIA, CNPSO. |
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Título: |
Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013. |
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ISSN: |
1438-7948 |
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DOI: |
10.1007/s10142-013-0322-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. MenosAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Si... Mostrar Tudo |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
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024 7 $a10.1007/s10142-013-0322-7$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aFast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.$h[electronic resource]
260 $c2013
520 $aAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment.
650 $aFeijão
700 1 $aRODRIGUES, E. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aOLIVEIRA, A. L. M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 13, n. 2, p. 275-283, Jun. 2013.
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