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| Registros recuperados : 72 | |
| 6. |  | GRANATE, M. J.; SEDIYAMA, M. A. N.; OLIVEIRA, L. R. de; CRUZ, C. D.; PUIATTI, M. Clonal selection in arracacha breeding. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 4, n. 1, p. 105-110, Mar. 2004.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 7. | | OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.| Biblioteca(s): Embrapa Soja. |
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| 9. | | OLIVEIRA, L. R.; LUCINI, T.; SMANIOTTO, M. A.; PANIZZI, A. R. Nymph development and adult feeding on, and damage to, immature siliquae of canola by different species of stink bugs. Neotropical Entomology, v. 52, n. 4, p. 621-628, Aug. 2023.| Biblioteca(s): Embrapa Trigo. |
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| 14. | | FERNANDES, A. A.; MARTINEZ, H. E. P.; OLIVEIRA, L. R. de. Produtividade, qualidade dos frutos e estado nutricional de plantas de pepino, cultivadas em hidroponia, em função das fontes de nutrientes. Horticultura Brasileira, Brasília, v. 20, n. 4, p. 571-573, dez. 2002.| Biblioteca(s): Embrapa Hortaliças. |
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| 15. | | OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013.| Biblioteca(s): Embrapa Soja. |
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| 16. | | LEITE, J. P.; REIS, O. V. dos; TABOSA, J. N.; OLIVEIRA, L. R. de. Efeitos residuais de N, P e K em podzolico amarelo com sorgo forrageiro (Sorghum bicolor (L.) Moench), sob condicoes de sequeiro, em rotacao com batateira (Solanum tuberosum L.). Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 1, n. 3, p. 38-46, set./dez. 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | OLIVEIRA, B. C. S. de; ARAUJO, A. C. de; OLIVEIRA, L. R. de; MANZI, A. O. Caracterização da variação diária e sazonal do CO2 atmosférico em cultivo da palma de óleo com híbridos interespecíficos (Elaeis guineensis X Elaeis oleifera) no leste da Amazônia. In: WORKSHOP BRASILEIRO DE MICROMETEOROLOGIA, 9., 2015, Santa Maria, RS. Anais. Santa Maria, RS: UFSM, 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | NAMORATO, H.; MIRANDA, G. V.; SOUZA, L. V. de; OLIVEIRA, L. R.; DELIMA, R. O.; MANTOVANI, E. E. Comparing Biplot Multivariate Analyses with Eberhart and Russell’ method for genotype x environment interaction. Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 4, p. 299-307, Dec. 2009.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 72 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
19/03/2010 |
|
Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL - CNPq; FRANCISMAR CORREA MARCELINO, CNPSo; FERNANDO GOMES BARCELLOS, CNPSo; ELISETE PAINS RODRIGUES, CNPSo - CNPq; MANUEL MEGIAS, Universidad de Sevilha; MARIANGELA HUNGRIA DA CUNHA, CNPSo. |
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Título: |
The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010. |
|
DOI: |
DOI 10.1007/s10142-009-0151-x |
|
Idioma: |
Inglês |
|
Conteúdo: |
Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed. |
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Palavras-Chave: |
Reverse transcription quantitative-PCR. |
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Thesagro: |
Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose. |
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Thesaurus NAL: |
Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis. |
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Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
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008 2010 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s10142-009-0151-x$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aThe nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.
260 $c2010
520 $aRhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
650 $aBeans
650 $aGenes
650 $aNitrogen fixation
650 $aRhizobium tropici
650 $aSymbiosis
650 $aFeijao
650 $aFixacao de nitrogenio
650 $aGene
650 $aPhaseolus Vulgaris
650 $aSimbiose
653 $aReverse transcription quantitative-PCR
700 1 $aMARCELINO, F. C.
700 1 $aBARCELLOS, F. G.
700 1 $aRODRIGUES, E. P.
700 1 $aMEGIAS, M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics, Heidelberg$gv. 10, n. 3, p. 425-431, Aug. 2010.
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