Data/hora: 30/05/2024 12:46:23

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	29/01/2009	
Data da última atualização: 	24/05/2018	
Autoria: 	WOLFF, J. L.; VALICENTE, F. H.; MARTINS, R.; OLIVEIRA, J. V. de C.; ZANOTTO, P. M. de A.	
Afiliação: 	José Luiz Caldas Wolff, Universidade de Mogi das Cruzes; FERNANDO HERCOS VALICENTE, CNPMS; Renata Martins, Universidade de Mogi das Cruzes; Juliana Velasco de Castro Oliveira, USP; Paolo Marinho de andrade Zanotto, USP.	
Título: 	Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	Journal of General Virology, Cambridge, v. 89, n. 6, p. 1202-1211, 2008.	
DOI: 	10.1099/vir.0.83581-0	
Idioma: 	Inglês	
Conteúdo: 	The genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative of temporal organization in the gene accretion processo.	
Thesagro: 	Praga de planta.	
Categoria do assunto: 	O Insetos e Entomologia	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (21256-1) - UPC - AP - DD -  -  -  - DIS