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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja; Embrapa Suínos e Aves. |
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Data corrente: |
11/11/2020 |
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Data da última atualização: |
18/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, E.; GRAÇA, J. P. da; PORTO, C.; PRADO, R. M.; HOFFMANN-CAMPO, C. B.; MEYER, M. C.; NUNES, E. de O.; PILAU, E. J. |
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Afiliação: |
Evandro Silva, Universidade Estadual de Maringá, UEM, Maringá, PR.; José Perez da Graça, Universidade Estadual de Maringá, UEM, Maringá, PR.; Carla Porto, Universidade Estadual de Maringá, UEM, Maringá, PR.; Rodolpho Martin do Prado, Universidade Estadual de Maringá, UEM, Maringá, PR.; CLARA BEATRIZ HOFFMANN CAMPO, CNPSO; MAURICIO CONRADO MEYER, CNPSO; ESTELA DE OLIVEIRA NUNES, CNPSA; Eduardo Jorge Pilau, Universidade Estadual de Maringá, UEM, Maringá, PR. |
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Título: |
Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, n. 138, 2020. |
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Páginas: |
11 p. |
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DOI: |
10.1038/s41598-019-56782-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen. MenosAbstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of ... Mostrar Tudo |
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Palavras-Chave: |
Espectrometria de massa; Ferrugem asiática. |
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Thesagro: |
Ferrugem; Soja. |
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Thesaurus Nal: |
Mass spectrometry; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02549naa a2200301 a 4500
001 2126485
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1038/s41598-019-56782-4$2DOI
100 1 $aSILVA, E.
245 $aUnraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aAbstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.
650 $aMass spectrometry
650 $aSoybean rust
650 $aFerrugem
650 $aSoja
653 $aEspectrometria de massa
653 $aFerrugem asiática
700 1 $aGRAÇA, J. P. da
700 1 $aPORTO, C.
700 1 $aPRADO, R. M.
700 1 $aHOFFMANN-CAMPO, C. B.
700 1 $aMEYER, M. C.
700 1 $aNUNES, E. de O.
700 1 $aPILAU, E. J.
773 $tScientific Reports$gv. 10, n. 138, 2020.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
29/01/2024 |
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Data da última atualização: |
29/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOBRAL, G. de C.; OLIVEIRA, J. S. de; SANTOS, E. M.; ARAUJO, G. G. L. de; SANTOS, F. N. de S.; CAMPOS. F. S.; CAVALCANTI, H. S.; VIEIRA, D. de S.; LEITE, G. M.; COELHO, D. F. O.; SANTANA, L. P.; GOMES, P. G. B.; TORRES JÚNIOR, P. da C.; SANTOS, M. A. C.; VIANA, N. B. |
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Afiliação: |
GILBERTO DE CARVALHO SOBRAL, Universidade Federal da Paraíba, Areia, PB; JULIANA SILVA DE OLIVEIRA, Universidade Federal da Paraíba, Areia, PB; EDSON MAURO SANTOS, Universidade Federal da Paraíba, Areia, PB; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; FRANCISCO NAYSSON DE SOUSA SANTOS, Universidade Federal do Maranhão, Chapadinha, Maranhão; FLEMING SENA CAMPOS, Universidade Federal do Maranhão, Chapadinha, Maranhão; HACTUS SOUTO CAVALCANTI, Universidade Federal do Maranhão, Chapadinha, Maranhão; DIEGO DE SOUZA VIEIRA, Universidade Federal da Paraíba, Areia, Paraiba; GUILHERME MEDEIROS LEITE, Universidade Federal da Paraíba, Areia, Paraiba; DIEGO FRANCISCO OLIVEIRA COELHO, Universidade Federal da Paraíba, Areia, Paraiba; LILIANE PEREIRA SANTANA, UFRPE; PALOMA GABRIELA BATISTA GOMES, Universidade Federal da Paraíba, Areia, Paraiba; PAULO DA CUNHA TORRES JÚNIOR, Universidade Federal da Paraíba, Areia, Paraiba; MARIA ALYNE COUTINHO SANTOS, Universidade Federal da Paraíba, Areia, Paraiba; NELQUIDES BRAZ VIANA, Universidade Federal da Paraíba, Areia, Paraiba. |
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Título: |
Optimizing silage quality in drylands: corn stover and forage cactus mixture on nutritive value, microbial activity, and aerobic stability. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Arid Environments, v. 220, 105123, 2024. |
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DOI: |
https://doi.org/10.1016/j.jaridenv.2024.105123 |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to determine the optimal ratio for the mixture of forage cactus and corn stover silage, emphasizing the evaluation of fermentative profile, microbial populations, dry matter losses, chemical composition, and aerobic stability. A completely randomized design with four treatments and five replications was employed in this study. Treatments included varying proportions of forage cactus in corn stover at ensiling (0%, 10%, 20%, and 40% of fresh matter). After a 100-day fermentation period, the assayed variables underwent regression analysis. Forage cactus inclusion led to linear dry matter and ether extract reductions, with crude protein showing a negative quadratic effect (p < 0.05). The pH and water-soluble carbohydrates exhibited negative quadratic effects with the increasing proportion of forage cactus (p < 0.05). Lactic and acetic acids exhibited quadratic effects, reaching their peaks at approximately 20% forage cactus (p < 0.05). Lactic acid bacteria demonstrated a linear decrease, while yeasts/molds displayed a negative quadratic effect (p < 0.05). Dry matter losses followed a quadratic pattern, with the minimum values observed at 20% forage cactus (p < 0.05). Additionally, the inclusion of forage cactus significantly enhanced aerobic stability (p < 0.05). The incorporation of 20% forage cactus into corn stover silage markedly improved its quality. |
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Palavras-Chave: |
Aditivo nutricional; Atividade microbiana; Estabilidade aeróbica; Heterolático; Palha de milho. |
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Thesagro: |
Fermentação; Opuntia Ficus Indica; Palma Forrageira; Silagem; Valor Nutritivo. |
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Thesaurus NAL: |
Animal nutrition; Fermentation; Opuntia; Pastures. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161408/1/Optimizing-silage-quality-in-drylands-corn-stover-and-forage-cactus-2024.pdf
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Marc: |
LEADER 02812naa a2200469 a 4500
001 2161408
005 2024-01-29
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jaridenv.2024.105123$2DOI
100 1 $aSOBRAL, G. de C.
245 $aOptimizing silage quality in drylands$bcorn stover and forage cactus mixture on nutritive value, microbial activity, and aerobic stability.$h[electronic resource]
260 $c2024
520 $aThe study aimed to determine the optimal ratio for the mixture of forage cactus and corn stover silage, emphasizing the evaluation of fermentative profile, microbial populations, dry matter losses, chemical composition, and aerobic stability. A completely randomized design with four treatments and five replications was employed in this study. Treatments included varying proportions of forage cactus in corn stover at ensiling (0%, 10%, 20%, and 40% of fresh matter). After a 100-day fermentation period, the assayed variables underwent regression analysis. Forage cactus inclusion led to linear dry matter and ether extract reductions, with crude protein showing a negative quadratic effect (p < 0.05). The pH and water-soluble carbohydrates exhibited negative quadratic effects with the increasing proportion of forage cactus (p < 0.05). Lactic and acetic acids exhibited quadratic effects, reaching their peaks at approximately 20% forage cactus (p < 0.05). Lactic acid bacteria demonstrated a linear decrease, while yeasts/molds displayed a negative quadratic effect (p < 0.05). Dry matter losses followed a quadratic pattern, with the minimum values observed at 20% forage cactus (p < 0.05). Additionally, the inclusion of forage cactus significantly enhanced aerobic stability (p < 0.05). The incorporation of 20% forage cactus into corn stover silage markedly improved its quality.
650 $aAnimal nutrition
650 $aFermentation
650 $aOpuntia
650 $aPastures
650 $aFermentação
650 $aOpuntia Ficus Indica
650 $aPalma Forrageira
650 $aSilagem
650 $aValor Nutritivo
653 $aAditivo nutricional
653 $aAtividade microbiana
653 $aEstabilidade aeróbica
653 $aHeterolático
653 $aPalha de milho
700 1 $aOLIVEIRA, J. S. de
700 1 $aSANTOS, E. M.
700 1 $aARAUJO, G. G. L. de
700 1 $aSANTOS, F. N. de S.
700 1 $aCAMPOS. F. S.
700 1 $aCAVALCANTI, H. S.
700 1 $aVIEIRA, D. de S.
700 1 $aLEITE, G. M.
700 1 $aCOELHO, D. F. O.
700 1 $aSANTANA, L. P.
700 1 $aGOMES, P. G. B.
700 1 $aTORRES JÚNIOR, P. da C.
700 1 $aSANTOS, M. A. C.
700 1 $aVIANA, N. B.
773 $tJournal of Arid Environments$gv. 220, 105123, 2024.
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