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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
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Título: |
Vertical transmissibility of small ruminant lentivirus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
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Palavras-Chave: |
SRLV. |
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Thesaurus Nal: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
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Marc: |
LEADER 02915naa a2200325 a 4500
001 2127175
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI
100 1 $aARAÚJO, J. F.
245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource]
260 $c2020
520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
650 $aGenetic techniques and protocols
650 $aGoat diseases
650 $aGoats
650 $aLentivirus
650 $aSheep diseases
653 $aSRLV
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aSIDER, L. H.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aDAMASCENO, E. M.
700 1 $aSOUZA, S. C. R.
700 1 $aTEIXEIRA, M. F. da S.
773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/04/2012 |
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Data da última atualização: |
24/04/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ASSIS, V. L.; STOCK, L. A.; OLIVEIRA, J. S. e; LOPES, F. C. F. |
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Afiliação: |
Vítor Lopes de Assis; LORILDO ALDO STOCK, CNPGL; JACKSON SILVA E OLIVEIRA, CNPGL; FERNANDO CESAR FERRAZ LOPES, CNPGL. |
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Título: |
Viabilidade econômica da inclusão da glicerina bruta oriunda da produção de biodiesel em dietas de vacas em lactação. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: Workshop de Iniciação Científica da Embrapa Gado de Leite, 8., 2011, Juiz de Fora. Anais... |
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Série: |
Documentos, 151. |
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Idioma: |
Português |
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Palavras-Chave: |
Viabilidade econômica. |
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Thesagro: |
Alimentação; Glicerina. |
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Thesaurus NAL: |
biodiesel. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 00676naa a2200205 a 4500
001 1922910
005 2012-04-24
008 2011 bl uuuu u00u1 u #d
100 1 $aASSIS, V. L.
245 $aViabilidade econômica da inclusão da glicerina bruta oriunda da produção de biodiesel em dietas de vacas em lactação.
260 $c2011
490 $aDocumentos, 151.
650 $abiodiesel
650 $aAlimentação
650 $aGlicerina
653 $aViabilidade econômica
700 1 $aSTOCK, L. A.
700 1 $aOLIVEIRA, J. S. e
700 1 $aLOPES, F. C. F.
773 $tIn: Workshop de Iniciação Científica da Embrapa Gado de Leite, 8., 2011, Juiz de Fora. Anais...
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Embrapa Gado de Leite (CNPGL) |
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