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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
29/03/2018 |
Data da última atualização: |
29/03/2018 |
Autoria: |
SANTOS, E. A. A.; LIMA, G. L.; PRAXEDES, E. C. G.; SILVA, A. M.; MAIA, K. M.; OLIVEIRA, M. F.; RODRIGUES, A. P. R.; SILVA, A. R. |
Afiliação: |
Erica A. A. Santos, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Gabriela L. Lima, Departamento de Medicina Veterinária/Universidade Federal de Roraima; Erica C. G. Praxedes, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Andréia M. Silva, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Keilla M. Maia, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Moacir F. Oliveira, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Ana Paula R. Rodrigues, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais/Universidade Estadual do Ceará; Alexandre R. Silva, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa. |
Título: |
Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina). |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 175-182, janeiro 2018. |
Idioma: |
Inglês |
Notas: |
Título em português: Morfometria, estimativa e ultraestrutura da população folicular ovariana preantral de cutia (Dasyprocta leporina). |
Conteúdo: |
The aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation. MenosThe aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyt... Mostrar Tudo |
Palavras-Chave: |
Dasyprocta leporine; Folículos; Morfometria; Ovarian tissue; População folicular ovariana preantral; Tecido ovariano; Ultraestrutura. |
Thesagro: |
Cutia. |
Thesaurus Nal: |
Agouti; Morphometry; Ovarian follicles; Ultrastructure. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174763/1/Estimation-morphometry-and-ultrastructure-of-ovarian-peantral.pdf
|
Marc: |
LEADER 03064naa a2200361 a 4500 001 2089954 005 2018-03-29 008 2018 bl uuuu u00u1 u #d 100 1 $aSANTOS, E. A. A. 245 $aEstimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina).$h[electronic resource] 260 $c2018 500 $aTítulo em português: Morfometria, estimativa e ultraestrutura da população folicular ovariana preantral de cutia (Dasyprocta leporina). 520 $aThe aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation. 650 $aAgouti 650 $aMorphometry 650 $aOvarian follicles 650 $aUltrastructure 650 $aCutia 653 $aDasyprocta leporine 653 $aFolículos 653 $aMorfometria 653 $aOvarian tissue 653 $aPopulação folicular ovariana preantral 653 $aTecido ovariano 653 $aUltraestrutura 700 1 $aLIMA, G. L. 700 1 $aPRAXEDES, E. C. G. 700 1 $aSILVA, A. M. 700 1 $aMAIA, K. M. 700 1 $aOLIVEIRA, M. F. 700 1 $aRODRIGUES, A. P. R. 700 1 $aSILVA, A. R. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 1, p. 175-182, janeiro 2018.
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Roraima. |
Data corrente: |
17/11/1997 |
Data da última atualização: |
06/09/2022 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
LUZ, F. J. de F.; OLIVEIRA, J. M. F. de. |
Afiliação: |
FRANCISCO JOACI DE FREITAS LUZ, CPAF-RR; JANE MARIA FRANCO DE OLIVEIRA, CPAF-RR. |
Título: |
Orientações técnicas para o cultivo de cenoura em Roraima. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Boa Vista, RR: EMBRAPA-CPAF/Roraima, 1997. |
Páginas: |
8 p. |
Série: |
(EMBRAPA-CPAF/Roraima. Circular tecnica, 2). |
ISSN: |
0101-9813 |
Idioma: |
Português |
Conteúdo: |
Clima, cultivares, escolha do local, preparo do solo, adubacao e correcao do solo, adubacao organica, adubacao quimica, adubacao de cobertura, correcao do solo, semeadura, praticas culturais, desbaste, irrigacao, capinas, rotacao de cultura, controle de pragas e doencas, pragas,doencas, colheita e embalagem, referencias bibliograficas.; |
Palavras-Chave: |
Brasil; Carrot; Cenoura - cultivo - Brasil - Roraima; Control; Controle; Correcao; Correction; Crop system; Cultivation; Cultive; Cultivo; Culture; Disease; Farm system; Harvest; Manuring; Pest; Praticas culturais; Preparo da area; Roraima; Seding. |
Thesagro: |
Adubação; Arroz; Cenoura; Clima; Colheita; Daucus Carota; Doença; Embalagem; Praga; Semeadura; Sistema de Cultivo; Sistema de Produção; Solo. |
Thesaurus NAL: |
agriculture; Amazonia; Brazil; carrots; packaging; rice; soil. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202135/1/cit00297-PDF.pdf
|
Marc: |
LEADER 01866nam a2200649 a 4500 001 1695132 005 2022-09-06 008 1997 bl uuuu u0uu1 u #d 022 $a0101-9813 100 1 $aLUZ, F. J. de F. 245 $aOrientações técnicas para o cultivo de cenoura em Roraima. 260 $aBoa Vista, RR: EMBRAPA-CPAF/Roraima$c1997 300 $a8 p. 490 $a(EMBRAPA-CPAF/Roraima. Circular tecnica, 2). 520 $aClima, cultivares, escolha do local, preparo do solo, adubacao e correcao do solo, adubacao organica, adubacao quimica, adubacao de cobertura, correcao do solo, semeadura, praticas culturais, desbaste, irrigacao, capinas, rotacao de cultura, controle de pragas e doencas, pragas,doencas, colheita e embalagem, referencias bibliograficas.; 650 $aagriculture 650 $aAmazonia 650 $aBrazil 650 $acarrots 650 $apackaging 650 $arice 650 $asoil 650 $aAdubação 650 $aArroz 650 $aCenoura 650 $aClima 650 $aColheita 650 $aDaucus Carota 650 $aDoença 650 $aEmbalagem 650 $aPraga 650 $aSemeadura 650 $aSistema de Cultivo 650 $aSistema de Produção 650 $aSolo 653 $aBrasil 653 $aCarrot 653 $aCenoura - cultivo - Brasil - Roraima 653 $aControl 653 $aControle 653 $aCorrecao 653 $aCorrection 653 $aCrop system 653 $aCultivation 653 $aCultive 653 $aCultivo 653 $aCulture 653 $aDisease 653 $aFarm system 653 $aHarvest 653 $aManuring 653 $aPest 653 $aPraticas culturais 653 $aPreparo da area 653 $aRoraima 653 $aSeding 700 1 $aOLIVEIRA, J. M. F. de
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