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| 145. | | MARTINS, L. H. S.; LOPES, A. S.; CARVALHO, A. V.; RODRIGUES, A. M. C.; OLIVEIRA, J. A. R. Análises físicas e caracterização físico-química de snack de mandioca (Manihot esculenta Crantz) e camarão regional (macrobrachium amazonicum). In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 8., 2009, Campinas. Ciência de alimentos no mundo globalizado: novos desafios, novas perspectivas. Campinas: Unicamp, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 146. | | OLIVEIRA, J. A; LOPES, J. G.; BARBOSA, B. D. R.; CORREIA, R. C.; MELO, N. F. de. Avaliação de substratos e níveis de disponibilidade de água na aclimatização de plantas micropropagadas de palma Orelha de elefante (Opuntia strica Hall). In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 13., 2018, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2018. p. 293-297. (Embrapa Semiárido. Documentos, 283).| Biblioteca(s): Embrapa Semiárido. |
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| 149. | | SARTORI, D. E. L.; OLIVEIRA, J. A. V. de; BRONDANI, C.; VIANELLO, R. P.; MENDONÇA, J. A. Avaliação dos genes Rubisco e AVP na cultivar de arroz (Oryza sativa) em dois níveis de fertilidade de solo. In: REUNIÃO CENTRO-OESTE DE CIÊNCIA DO SOLO, 5.; SIMPÓSIO DE NUTRIÇÃO DE PLANTAS NO CERRADO, 2., 2018, Goiânia. Uso eficiente de nutrientes e adubação de sistemas agrícolas: resumos. Goiânia: UFG, 2018. p. 365-366.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 152. | | MARTINS, L. H. S.; LOPES, A. S.; CARVALHO, A. V.; RODRIGUES, A. M. C.; OLIVEIRA, J. A. R. Avaliação sensorial de snack de uma mistura a base de mandioca e camarão regional. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 8., 2009, Campinas. Ciência de alimentos no mundo globalizado: novos desafios, novas perspectivas. Campinas: Unicamp, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 154. | | ALENCAR, M. M. de; OLIVEIRA, J. A. L.; FERRAZ FILHO, P. B.; BRULE, A. O.; SOUZA, J. C. Repetibilidade dos pesos e ganho de peso do nascimento a desmama de bezerros da raça Nelore. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 29., 1992, Lavras, MG. Anais... Lavras: SBZ, 1992. p. 91.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 155. | | OLIVEIRA, J. A. R. de; CARVALHO, A. V.; MOREIRA, D. K. T.; MARTINS, L. H. da S. Elaboração e caracterização de estruturado obtido de polpa concentrada de cupuaçu. Revista de Ciências Agrárias, Belém, PA, v. 53, n. 2, p. 164-170, jul./dez. 2010.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 160. | | OLIVEIRA, J. A. V. de; ABREU, F. R. M.; MENDONÇA, J. A.; VIANELLO, R. P.; BRONDANI, C. Expressão relativa de genes homólogos de Arabidopsis em arroz relacionados ao aumento da produtividade. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 87. (Embrapa Arroz e Feijão. Documentos, 311).| Biblioteca(s): Embrapa Arroz e Feijão. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
05/06/2018 |
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Data da última atualização: |
30/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ABREU, F. R. M.; DEDICOVA, B.; VIANELLO, R. P.; LANNA, A. C.; OLIVEIRA, J. A. V. de; VIEIRA, A. F.; MORAIS, O. P.; MENDONÇA, J. A.; BRONDANI, C. |
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Afiliação: |
FERNANDA RAQUEL MARTINS ABREU, UFG; BEATA DEDICOVA, CIAT; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; JOAO AUGUSTO VIEIRA DE OLIVEIRA, UFG; ARIADNA FARIA VIEIRA, UFG; ODILON PEIXOTO MORAIS; JOAO ANTONIO MENDONCA, CNPAF; CLAUDIO BRONDANI, CNPAF. |
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Título: |
Overexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Protoplasma, v. 255, n. 6, p. 1751-1761, Nov. 2018. |
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ISSN: |
1615-6102 |
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DOI: |
10.1007/s00709-018-1265-6 |
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Idioma: |
Inglês |
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Conteúdo: |
This work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in a reduction in production loss under severe water deficit and revealed a possibility for the development of upland rice cultivars that are more tolerant to extreme drought conditions. MenosThis work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in... Mostrar Tudo |
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Thesagro: |
Arroz; Engenharia Genética; Oryza Sativa; Resistência a Seca; Stress. |
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Thesaurus NAL: |
Abiotic stress; Drought tolerance; Gene expression; Genetic engineering; Phospholipases; Rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02737naa a2200373 a 4500
001 2092221
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
022 $a1615-6102
024 7 $a10.1007/s00709-018-1265-6$2DOI
100 1 $aABREU, F. R. M.
245 $aOverexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.).$h[electronic resource]
260 $c2018
520 $aThis work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in a reduction in production loss under severe water deficit and revealed a possibility for the development of upland rice cultivars that are more tolerant to extreme drought conditions.
650 $aAbiotic stress
650 $aDrought tolerance
650 $aGene expression
650 $aGenetic engineering
650 $aPhospholipases
650 $aRice
650 $aArroz
650 $aEngenharia Genética
650 $aOryza Sativa
650 $aResistência a Seca
650 $aStress
700 1 $aDEDICOVA, B.
700 1 $aVIANELLO, R. P.
700 1 $aLANNA, A. C.
700 1 $aOLIVEIRA, J. A. V. de
700 1 $aVIEIRA, A. F.
700 1 $aMORAIS, O. P.
700 1 $aMENDONÇA, J. A.
700 1 $aBRONDANI, C.
773 $tProtoplasma$gv. 255, n. 6, p. 1751-1761, Nov. 2018.
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