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| Registros recuperados : 36 | |
| 9. |  | OLIVEIRA, G. S.; ARF, O.; SA, M. E.; RODRIGUES, R. A. F. Efeito de espacamento e densidades de semeadura na qualidade fisiologica das sementes de cultivares de arroz de sequeiro irrigados por aspersao. Informativo ABRATES, Brasilia, v.7, n.1/2, p.166, 1997. Trabalho apresentado no 10o. Congresso Brasileiro de Sementes, 1997. Resumo.| Biblioteca(s): Embrapa Cerrados. |
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| 10. | | MOURA, M. T.; PAULINI, F.; OLIVEIRA, G. S.; MELO, E. de O.; RUMPF, R. Expressão da proteína fluorescente verde (EGFP) em fibroblastos de bovinos adultos da raça Junqueira. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 213.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | OLIVEIRA, G. S. de; SILVEIRA, E. S.; SANTOS, M. F.; FERNANDES, C. D.; JANK, L.; MATSUBARA, E. T. Caracterização do banco de germoplasma de Panicum maximum quanto a fatores bióticos: uso de drones para avaliação de doenças. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 15., 2019, Campo Grande, MS. [Resumos dos trabalhos...]. Brasília, DF: Embrapa, 2019 80 p. (Embrapa Gado de Corte. Documentos, 264). Comitê Organizador: Marlene de Barros Coelho; Lenita Ramires dos Santos; Rodrigo Carvalho Alva; Lucimara Chiari; Thais Basso Amaral. p. 40-41| Biblioteca(s): Embrapa Gado de Corte. |
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| 13. | | NOGUEIRA, C. M. D.; MAIA, G, A.; OLIVEIRA, G. S. F. de; FIGUEIREDO, R. W. de; ORIA, H. F. Caracteristicas fisicas, fisico-quimicas e quimicas de acerolas do clone UFC Ciencia Agronomica, v.27, n.1/2, p.76-80, Fortaleza, CE, 1996| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | ARAÚJO, V. G. B.; PAULINI, F.; OLIVEIRA, G. S.; RUA, P. J. S.; FRANCO, M. M.; MELO, E. de O. Produção de anticorpos específicos para os hormônios GDF9 e BMP15 de bovinos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 212.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | LIMA, S. R. de; OLIVEIRA, G. S. de; MORAIS, S. A. L. de; NASCIMENTO, E. A. do; CHANG, R. Estudo dos constituintes macromoleculares, extrativos voláteis e compostos fenólicos da madeira de candeia - Moquinia polymorpha (Less.) DC. Ciência Florestal, Santa Maria, v. 17, n. 1/2, p. 145-155, jan./mar. 2007.| Biblioteca(s): Embrapa Florestas. |
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| 17. | | OLIVEIRA, G. S.; SOUZA, T. L.; BARRETO, B. M. C. dos S.; SIGNOR, D.; FERNANDES JUNIOR, P. I. Diversidade genética de bactérias isoladas de nódulos de feijão-caupi cultivado em solos com e sem a aplicação de biochar. In: JORNADA DE INTEGRAÇÃO DA PÓS-GRADUAÇÃO DA EMBRAPA SEMIÁRIDO, 2., 2017, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2017. p. 313-319. (Embrapa Semiárido. Documentos, 280).| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | NOGUEIRA, C. M. D.; MAIA, G. A.; FIGUEIREDO, R. W. de; OLIVEIRA, G. S. F. de; ORIA, H. F. Estabilidade fisico-quimica e quimica da polpa de acerola Revista Brasileira de Fruticultura, v.19, n.1, p.139-144, Cruz das Almas, 1997| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | PIMENTEL, M. L.; MAIA, G. A.; OLIVEIRA, G. S. F. de; MONTEIRO, J. C. S.; SILVA JUNIOR, A. Influencia do processamento sobre a vitamina C do suco da acerola (Malpighia glabra L.).1 Revista Brasileira de Fruticultura, Jaboticabal-SP, v.23, n.1, p.143-146, 2001| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | OLIVEIRA, G. S. de A.; FIGUEIRAS, S. da S.; SILVA, K. R. C.; SERRANO, L. J. P.; ALCANTARA, R. M. C. M. de; ARAUJO NETO, R. B. de. Análise do crescimento inicial de mudas florestais em substratos orgânicos. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 7., 2021, Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2023. (Embrapa Meio-Norte. Documentos, 287). p. 14.| Biblioteca(s): Embrapa Meio-Norte. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
22/06/2020 |
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Data da última atualização: |
22/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVEIRA, G. S.; SENA, P. T. S.; NASCIMENTO, T. R. do; FERREIRA NETO, R. A.; PEREIRA, J. R. C.; MARTINS, L. M. V.; FREITAS, A. D. S. de; SIGNOR, D.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
Gilmar Silva Oliveira; Pâmella Thalita Souza Sena; Tailane Ribeiro do Nascimento; Reginaldo Alves Ferreira Neto; Juliana Ribeiro Costa Pereira; Lindete Míria Vieira Martins; Ana Dolores Santiago de Freitas; DIANA SIGNOR DEON, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Are cowpea-nodulating Bradyrhizobial communities influenced by bibochar amendments in soils? genetic diversity and symbiotic effectiveness assessment of two agricultural soils of Brazilian drylands. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Soil Science and Plant Nutrition, v.20, n.2, p. 439-449, 2020. |
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DOI: |
https://doi.org/10.1007/s42729-019-00128-6 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the influence of biochar amendment on the bacterial genetic diversity and symbiosis in cowpea-nodulating rhizobial communities in two agricultural soils of Brazilian drylands. Vertisol and ultisol surface layer soil samples were collected in the Brazilian semiarid region. The soils were mixed with biochar and cowpea seeds (BRS Marataoã) and sown in pots. Forty-five days after plant emergence, the nodule bacteria were isolated. The genetic variability of the nodule bacteria was evaluated by means of restriction fragment length polymorphism of 16S-23S rRNA intergenic spacer region (IGS-RFLP), and those that shared identical IGS-RFLP profiles were fingerprinted by BOX-PCR. By selecting of representative isolates in the genetic clusters, 27 bacteria were identified by 16S-23S IGS sequencing and symbiotically assessed. The IGS-RFLP dendrogram of the 73 isolates had 20 clusters; the multivariate correspondence analysis grouped both Vertisol treatments, and the Ultisol treatments were placed far away in the biplot. Ultisol showed higher bradyrhizobial diversity than the Vertisol. The fingerprinting indicated the presence of only three clonal colonies, showing high intraspecific diversity. The 16S-23S IGS sequences indicated the prevalence of bacteria related to Bradyrhizobium guangxiense in the Vertisol and Bradyrhizobium zhanjiangense in the Ultisol, in addition to the presence of three putative Microvirga spp. in the Ultisol. The symbiotic efficiency of 22 out of 27 bacteria was comparable with that of the inoculant strain Bradyrhizobium yuanmingense BR 3267. The genetic diversity of the cowpea-nodulating bradyrhizobia was not primarily affected by the biochar application in two agricultural soils of Brazilian drylands. Both soils harbored symbiotically efficient bacteria, without the influence of biochar amendment MenosThe objective of this study was to evaluate the influence of biochar amendment on the bacterial genetic diversity and symbiosis in cowpea-nodulating rhizobial communities in two agricultural soils of Brazilian drylands. Vertisol and ultisol surface layer soil samples were collected in the Brazilian semiarid region. The soils were mixed with biochar and cowpea seeds (BRS Marataoã) and sown in pots. Forty-five days after plant emergence, the nodule bacteria were isolated. The genetic variability of the nodule bacteria was evaluated by means of restriction fragment length polymorphism of 16S-23S rRNA intergenic spacer region (IGS-RFLP), and those that shared identical IGS-RFLP profiles were fingerprinted by BOX-PCR. By selecting of representative isolates in the genetic clusters, 27 bacteria were identified by 16S-23S IGS sequencing and symbiotically assessed. The IGS-RFLP dendrogram of the 73 isolates had 20 clusters; the multivariate correspondence analysis grouped both Vertisol treatments, and the Ultisol treatments were placed far away in the biplot. Ultisol showed higher bradyrhizobial diversity than the Vertisol. The fingerprinting indicated the presence of only three clonal colonies, showing high intraspecific diversity. The 16S-23S IGS sequences indicated the prevalence of bacteria related to Bradyrhizobium guangxiense in the Vertisol and Bradyrhizobium zhanjiangense in the Ultisol, in addition to the presence of three putative Microvirga spp. in the Ultisol. The symbio... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Fixação biológica de nitrogênio; Microvirga; Semiárido. |
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Thesagro: |
Amendoim; Bactéria; Feijão; Rhizobium Cowpea. |
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Thesaurus NAL: |
Bradyrhizobium. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03030naa a2200337 a 4500
001 2123403
005 2020-06-22
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42729-019-00128-6$2DOI
100 1 $aOLIVEIRA, G. S.
245 $aAre cowpea-nodulating Bradyrhizobial communities influenced by bibochar amendments in soils? genetic diversity and symbiotic effectiveness assessment of two agricultural soils of Brazilian drylands.$h[electronic resource]
260 $c2020
520 $aThe objective of this study was to evaluate the influence of biochar amendment on the bacterial genetic diversity and symbiosis in cowpea-nodulating rhizobial communities in two agricultural soils of Brazilian drylands. Vertisol and ultisol surface layer soil samples were collected in the Brazilian semiarid region. The soils were mixed with biochar and cowpea seeds (BRS Marataoã) and sown in pots. Forty-five days after plant emergence, the nodule bacteria were isolated. The genetic variability of the nodule bacteria was evaluated by means of restriction fragment length polymorphism of 16S-23S rRNA intergenic spacer region (IGS-RFLP), and those that shared identical IGS-RFLP profiles were fingerprinted by BOX-PCR. By selecting of representative isolates in the genetic clusters, 27 bacteria were identified by 16S-23S IGS sequencing and symbiotically assessed. The IGS-RFLP dendrogram of the 73 isolates had 20 clusters; the multivariate correspondence analysis grouped both Vertisol treatments, and the Ultisol treatments were placed far away in the biplot. Ultisol showed higher bradyrhizobial diversity than the Vertisol. The fingerprinting indicated the presence of only three clonal colonies, showing high intraspecific diversity. The 16S-23S IGS sequences indicated the prevalence of bacteria related to Bradyrhizobium guangxiense in the Vertisol and Bradyrhizobium zhanjiangense in the Ultisol, in addition to the presence of three putative Microvirga spp. in the Ultisol. The symbiotic efficiency of 22 out of 27 bacteria was comparable with that of the inoculant strain Bradyrhizobium yuanmingense BR 3267. The genetic diversity of the cowpea-nodulating bradyrhizobia was not primarily affected by the biochar application in two agricultural soils of Brazilian drylands. Both soils harbored symbiotically efficient bacteria, without the influence of biochar amendment
650 $aBradyrhizobium
650 $aAmendoim
650 $aBactéria
650 $aFeijão
650 $aRhizobium Cowpea
653 $aDiversidade genética
653 $aFixação biológica de nitrogênio
653 $aMicrovirga
653 $aSemiárido
700 1 $aSENA, P. T. S.
700 1 $aNASCIMENTO, T. R. do
700 1 $aFERREIRA NETO, R. A.
700 1 $aPEREIRA, J. R. C.
700 1 $aMARTINS, L. M. V.
700 1 $aFREITAS, A. D. S. de
700 1 $aSIGNOR, D.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tJournal of Soil Science and Plant Nutrition$gv.20, n.2, p. 439-449, 2020.
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