Data/hora: 17/06/2024 12:01:56

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	02/01/2014	
Data da última atualização: 	12/05/2023	
Autoria: 	DOMINGUES, L. F.; GIGLIOTI, R.; FEITOSA, K. A.; FANTATTO, R. R.; RABELO, M. D.; OLIVEIRA, M. C. de S.; BECHARA, G. H.; OLIVEIRA, G. P. de; BARIONI JUNIOR, W.; CHAGAS, A. C. de S.	
Afiliação: 	LUCIANA FERREIRA DOMINGUES, Universidade Estadual Paulista; RODRIGO GIGLIOTI, Universidade Estadual Paulista; KARINA ALVES FEITOSA, Centro Universitário Central Paulista; RAFAELA REGINA FANTATTO, Centro Universitário Central Paulista; MARCIO DIAS RABELO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; GERVÁSIO HENRIQUE BECHARA, Centro Universitário Central Paulista; GILSON PEREIRA DE OLIVEIRA, Universidade Estadual Paulista; WALDOMIRO BARIONI JUNIOR, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE.	
Título: 	In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Veterinary Parasitology, v. 197, N. 1-8, p. 263-270, oct. 2013.	
DOI: 	10.1016/j.vetpar.2013.04.031	
Idioma: 	Inglês	
Conteúdo: 	The development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC?? and LC?? were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC?? and LC?? were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development test, the LC?? and LC?? were respectively 1.7 and 7.3 mg/mL for the aqueous extract and 0.019 and 0.086 mg/mL for bromelain. In the in vivo test, the general efficacies of the treatments in relation to the negative control were 22.6%, 42.2%, 3.65% and 89% for the aqueous extract, industrial pineapple residue, bromelain and positive control respectively. The transformed EPG values were 3.19 ± 0.59, 3.32 ± 0.25, 2.85 ± 0.66, 3.44 ± 0.50, 2.28 ± 0.93 and 2.75 ± 0.94 for the aqueous extract, industrial residue, bromelain, negative control I, positive control and negative control II respectively. The results for all the treated groups differed significantly (p<0.05) from the positive control, and although the residue presented efficacy of 42.2%, there was no statistical difference (p>0.05) in relation to the negative control. Therefore, both the aqueous extract and bromelain were effective in vitro, but showed reduced anthelmintic efficacy in vivo. For the pineapple residue, the 42.2% in vivo efficacy in reducing the EPG and the possibility of reducing environmental contamination through reuse of industrial residue indicate it can also be useful for control of this parasite.	
Palavras-Chave: 	Aqueous extract; Bromelain; Industrial residue.	
Thesaurus Nal: 	Cysteine proteinases; Phytotherapy.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (22252-1) - UPC - AP - DD - PROCI-2013.00199 - DOM - 2013.00199 - DIS