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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
10/01/2011 |
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Data da última atualização: |
10/01/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, A. C. F.; ANDREOTE, F. D.; RIGONATO, J.; FIORE, M. F.; MELO, I. S. de; ARAUJO, W. L. |
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Afiliação: |
Armando C. F. Dias, Centro de Pesquisas Biotecnológicas - USP; Fernando D. Andreote, ESALQ-USP; Janaina Rigonato, CENA-USP; Marli Fátima Fiore, CENA-USP; ITAMAR SOARES DE MELO, CNPMA; Welington Luiz Araújo, Universidade de Mogi das Cruzes. |
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Título: |
The bacterial diversity in a Brazilian non-disturbed mangrove sediment. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 98, n. 4, p. 541-551, 2010. |
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ISBN: |
10.1007/s10482-010-9471-z. |
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Idioma: |
Inglês |
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Conteúdo: |
The bacterial diversity present in sediments of a well-preserved mangrove in Ilha do Cardoso, located in the extreme south of São Paulo State coastline, Brazil, was assessed using culture-independent molecular approaches (denaturing gradient gel electrophoresis (DGGE) and analysis of 166 sequences from a clone library). The data revealed a bacterial community dominated by Alphaproteobacteria (40.36%of clones), Gammaproteobacteria (19.28% of clones) and Acidobacteria (27.71% of clones), while minor components of the assemblage were affiliated to Betaproteobacteria, Deltaproteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The clustering and redundancy analysis (RDA) based on DGGE were used to determine factors that modulate the diversity of bacterial communities in mangroves, such as depth, seasonal fluctuations, and locations over a transect area from the sea to the land. Profiles of specific DGGE gels showed that both dominant (universal Bacteria and Alphaproteobacteria) and low-density bacterial communities (Betaproteobacteria and Actinobacteria) are responsive to shifts in environmental factors. The location within the mangrove was determinant for all fractions of the community studied, whereas season was significant for Bacteria, Alphaproteobacteria, and Betaproteobacteria and sample depth determined the diversity of Alphaproteobacteria and Actinobacteria. |
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Thesagro: |
Biodiversidade; Mangue. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01948naa a2200205 a 4500
001 1872512
005 2011-01-10
008 2010 bl --- 0-- u #d
100 1 $aDIAS, A. C. F.
245 $aThe bacterial diversity in a Brazilian non-disturbed mangrove sediment.
260 $c2010
520 $aThe bacterial diversity present in sediments of a well-preserved mangrove in Ilha do Cardoso, located in the extreme south of São Paulo State coastline, Brazil, was assessed using culture-independent molecular approaches (denaturing gradient gel electrophoresis (DGGE) and analysis of 166 sequences from a clone library). The data revealed a bacterial community dominated by Alphaproteobacteria (40.36%of clones), Gammaproteobacteria (19.28% of clones) and Acidobacteria (27.71% of clones), while minor components of the assemblage were affiliated to Betaproteobacteria, Deltaproteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The clustering and redundancy analysis (RDA) based on DGGE were used to determine factors that modulate the diversity of bacterial communities in mangroves, such as depth, seasonal fluctuations, and locations over a transect area from the sea to the land. Profiles of specific DGGE gels showed that both dominant (universal Bacteria and Alphaproteobacteria) and low-density bacterial communities (Betaproteobacteria and Actinobacteria) are responsive to shifts in environmental factors. The location within the mangrove was determinant for all fractions of the community studied, whereas season was significant for Bacteria, Alphaproteobacteria, and Betaproteobacteria and sample depth determined the diversity of Alphaproteobacteria and Actinobacteria.
650 $aBiodiversidade
650 $aMangue
700 1 $aANDREOTE, F. D.
700 1 $aRIGONATO, J.
700 1 $aFIORE, M. F.
700 1 $aMELO, I. S. de
700 1 $aARAUJO, W. L.
773 $tAntonie van Leeuwenhoek$gv. 98, n. 4, p. 541-551, 2010.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2014 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, E. J. de; FERREIRA, C. F.; SANTOS, V. da S.; OLIVEIRA, G. A. F. |
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Afiliação: |
EDER JORGE DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; VANDERLEI DA SILVA SANTOS, CNPMF; G.A.F. OLIVEIRA, UFRB. |
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Título: |
Development of a cassava core collection based on single nucleotide polymorphism markers. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.13, n.3, p. 6472-85, August, 2014. |
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ISSN: |
1676-5680 |
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DOI: |
10.4238/2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphism (SNP) markers were used in the largest cassava (Manihot esculenta Crantz) germplasm collection from Brazil to develop core collections based on the maximization strategy. Subsets with 61, 64, 84, 128, 256, and 384 cassava accessions were selected and named PoHEU, MST64, PoRAN, MST128, MST256, and MST384, respectively. All the 798 alleles identified by 402 SNP markers in the entire collection were captured in all core collections. Only small alterations in the diversity parameters were observed for the different core collections compared with the complete collection. Because of the optimal adjustment of the validation parameters representative of the complete collection, the absence of genotypes with high genetic similarity and the maximization of the genetic distances between accessions of the PoHEU core collection, which contained 4.7% of the accessions of the complete collection, maximized the genetic conservation of this important cassava collection. Furthermore, the development of this core collection will allow concentrated efforts toward future characterization and agronomic evaluation of accessions to maximize the diversity and genetic gains in cassava breeding programs. |
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Thesagro: |
Mandioca; Marcador genético; Marcador molecular; Polimorfismo genético. |
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Thesaurus NAL: |
Cassava; Genetic markers; Single nucleotide polymorphism. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02035naa a2200265 a 4500
001 1995194
005 2023-05-26
008 2014 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/2014.$2DOI
100 1 $aOLIVEIRA, E. J. de
245 $aDevelopment of a cassava core collection based on single nucleotide polymorphism markers.$h[electronic resource]
260 $c2014
520 $aSingle nucleotide polymorphism (SNP) markers were used in the largest cassava (Manihot esculenta Crantz) germplasm collection from Brazil to develop core collections based on the maximization strategy. Subsets with 61, 64, 84, 128, 256, and 384 cassava accessions were selected and named PoHEU, MST64, PoRAN, MST128, MST256, and MST384, respectively. All the 798 alleles identified by 402 SNP markers in the entire collection were captured in all core collections. Only small alterations in the diversity parameters were observed for the different core collections compared with the complete collection. Because of the optimal adjustment of the validation parameters representative of the complete collection, the absence of genotypes with high genetic similarity and the maximization of the genetic distances between accessions of the PoHEU core collection, which contained 4.7% of the accessions of the complete collection, maximized the genetic conservation of this important cassava collection. Furthermore, the development of this core collection will allow concentrated efforts toward future characterization and agronomic evaluation of accessions to maximize the diversity and genetic gains in cassava breeding programs.
650 $aCassava
650 $aGenetic markers
650 $aSingle nucleotide polymorphism
650 $aMandioca
650 $aMarcador genético
650 $aMarcador molecular
650 $aPolimorfismo genético
700 1 $aFERREIRA, C. F.
700 1 $aSANTOS, V. da S.
700 1 $aOLIVEIRA, G. A. F.
773 $tGenetics and Molecular Research$gv.13, n.3, p. 6472-85, August, 2014.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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