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Registros recuperados : 37 | |
8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de. Desenvolvimento de SNP (Single-Nucleotide Polymorphism) para estudos de variação genética e estabelecimento de coleção nuclear em mamoeiro (Carica papayaL.). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura, 2014. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COSTA, J. L. C.; OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; SILVA, A. dos S.; YAMAGISHI, M. E. B. Minissatélites: novas ferramentas moleculares para o mamoeiro. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 915 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | COSTA, J. L.; OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; CARVALHO, F. M. de; DANTAS, J. L. L. Otimização de locos de microssatélites para diferentes espécies de Passiflora. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 4., 2010, Cruz das Almas. [Anais...] Cruz das Almas: Embrapa Mandioca e Fruticultura, 2010. (Embrapa Mandioca e Fruticultura. Documentos, 190). 1 CD-ROM. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, V. J. dos S. de; OLIVEIRA, E. J. de; DANTAS, J. L. L.; DANTAS, A. C. V. L.; CASTRO, J. A.; OLIVEIRA, G. A. F. de. Diversidade de linhagens de mamoeiro na geração F 2:4. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1194 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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19. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, E. J. de; SANTOS, V. da S.; FERREIRA, C. F.; OLIVEIRA, G. A. F.; SILVA, M. S. da; AGUILAR-VILDOSO, C. I. Grupos de diversidade genética em Manihot esculenta Crantz com base na análise discriminante de componentes principais. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. Melhoramento genético. Resumo 99. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de; CARVALHO, F. M. de C.; SILVA, A. dos S.; COSTA, J. L; DANTAS, J. L. L. Identificação de genótipos com alto nível de homozigosidade visando à obtenção de linhagens de mamoeiro por seleção assistida por marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 4., 2010, Cruz das Almas. [Anais...] Cruz das Almas: Embrapa Mandioca e Fruticultura, 2010. (Embrapa Mandioca e Fruticultura. Documentos, 190).1 CD-ROM. PDF. 014. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 37 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
28/07/2015 |
Data da última atualização: |
03/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CARMO, C. D.; SANTOS, D. B.; ALVES, L. B.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de. |
Afiliação: |
C. D. CARMO, UFRB; D. B. SANTOS, UFRB; L. B. ALVES, UFRB; G. A. F. OLIVEIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
Título: |
Development of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plant Molecular Biology Reporte, 18 April 2015. |
Páginas: |
14P. |
Idioma: |
Inglês |
Conteúdo: |
Cassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers. MenosCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as... Mostrar Tudo |
Palavras-Chave: |
Marcador molelular. |
Thesagro: |
Mandioca; Marcador genético. |
Thesaurus NAL: |
Cassava; Genetic markers. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02362naa a2200241 a 4500 001 2020697 005 2023-03-03 008 2015 bl uuuu u00u1 u #d 100 1 $aCARMO, C. D. 245 $aDevelopment of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava.$h[electronic resource] 260 $c2015 300 $a14P. 520 $aCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers. 650 $aCassava 650 $aGenetic markers 650 $aMandioca 650 $aMarcador genético 653 $aMarcador molelular 700 1 $aSANTOS, D. B. 700 1 $aALVES, L. B. 700 1 $aOLIVEIRA, G. A. F. 700 1 $aOLIVEIRA, E. J. de 773 $tPlant Molecular Biology Reporte, 18 April 2015.
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