| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
|
Data corrente: |
22/02/2011 |
|
Data da última atualização: |
10/03/2011 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BRANDALISE, M.; SEVERINO, F. E.; MALUF, M. P.; MAIA, I. G. |
|
Afiliação: |
MIRIAN PEREZ MALUF, SAPC. |
|
Título: |
The promoter of a gene encoding an isoflavon reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In.: PLANT CELL REPORTS, v. 28, p. 1699-1708, 2009. |
|
Idioma: |
Inglês |
|
Conteúdo: |
A cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags (EST) of the Brazilian coffee EST database. The CaIRL cDNA contains a single open reading frame of 946 nucleotides (nt) encoding 314 amino acids (predicted molecular weight of 34 kDa). Several features identified the predicted CaIRL protein as a new member of the PIP family of NADPH-dependent reductases. Expression studies demonstrated that CaIRL is expressed exclusively in coffee leaves and its transcript level is markedly increased in response to fungal infection and mechanical injury. Analysis of transgenic tobacco plants harboring a CaIRL 50-flanking region (862 nt) fused to uidA reporter gene (GUS) confirmed the responsiveness of the putative promoter to abiotic stress in wounded leaves. In turn, a 50 deletion to -404 completely abolished promoter activation by abiotic stimulus in transgenic plants. The lack of GUS expression in non-wounded leaf tissues in transgenic tobacco was in contrast to the basal level of CaIRL expression observed in non-stressed healthy coffee leaves. |
|
Palavras-Chave: |
Coffee; Isoflavone-like proteins; Promoter; Tissue-specificity. |
|
Thesaurus Nal: |
Gene expression. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29271/1/The-promoter.pdf
|
|
Marc: |
LEADER 01904naa a2200217 a 4500
001 1880372
005 2011-03-10
008 2009 bl uuuu u00u1 u #d
100 1 $aBRANDALISE, M.
245 $aThe promoter of a gene encoding an isoflavon reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves.$h[electronic resource]
260 $c2009
520 $aA cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags (EST) of the Brazilian coffee EST database. The CaIRL cDNA contains a single open reading frame of 946 nucleotides (nt) encoding 314 amino acids (predicted molecular weight of 34 kDa). Several features identified the predicted CaIRL protein as a new member of the PIP family of NADPH-dependent reductases. Expression studies demonstrated that CaIRL is expressed exclusively in coffee leaves and its transcript level is markedly increased in response to fungal infection and mechanical injury. Analysis of transgenic tobacco plants harboring a CaIRL 50-flanking region (862 nt) fused to uidA reporter gene (GUS) confirmed the responsiveness of the putative promoter to abiotic stress in wounded leaves. In turn, a 50 deletion to -404 completely abolished promoter activation by abiotic stimulus in transgenic plants. The lack of GUS expression in non-wounded leaf tissues in transgenic tobacco was in contrast to the basal level of CaIRL expression observed in non-stressed healthy coffee leaves.
650 $aGene expression
653 $aCoffee
653 $aIsoflavone-like proteins
653 $aPromoter
653 $aTissue-specificity
700 1 $aSEVERINO, F. E.
700 1 $aMALUF, M. P.
700 1 $aMAIA, I. G.
773 $tIn.: PLANT CELL REPORTS$gv. 28, p. 1699-1708, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Cerrados. |
|
Data corrente: |
02/12/2010 |
|
Data da última atualização: |
02/12/2010 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
FRIZZAS, M. R.; LIMA, G. S. de; OLIVEIRA, C. M. de. |
|
Afiliação: |
MARINA REGINA FRIZZAS, UNB; GUILHERME SENA DE LIMA, UNICEUB; CHARLES MARTINS DE OLIVEIRA, CPAC. |
|
Título: |
Diversidade de ordens oligo e megadiversidade da classe insecta associada ao estrato superior e inferior da vegetação de um fragmento de cerradão no Distrito Federal. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Thesagro: |
Cerrado; Inseto; Vegetação. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00671naa a2200181 a 4500
001 1868492
005 2010-12-02
008 2010 bl uuuu u00u1 u #d
100 1 $aFRIZZAS, M. R.
245 $aDiversidade de ordens oligo e megadiversidade da classe insecta associada ao estrato superior e inferior da vegetação de um fragmento de cerradão no Distrito Federal.
260 $c2010
300 $c1 CD-ROM.
650 $aCerrado
650 $aInseto
650 $aVegetação
700 1 $aLIMA, G. S. de
700 1 $aOLIVEIRA, C. M. de
773 $tIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
