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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
23/02/2018 |
Data da última atualização: |
22/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GARRETT, R. D.; GARDNER, T. A.; MORELLO, T. F.; MARCHAND, S.; BARLOW, J.; BLAS, D. E. de; FERREIRA, J. N.; LEES, A. C.; PARRY, L. |
Afiliação: |
Rachael D. Garrett, Boston University; Toby A. Gardner, Stockholm Environment Institute; Thiago Fonseca Morello, Universidade Federal do ABC; Sebastien Marchand, CERDI/Université Clermont Auvergne; Jos Barlow, Lancaster University; Driss Ezzine de Blas, CIRAD; JOICE NUNES FERREIRA, CPATU; Alexander C. Lees, Manchester Metropolitan University / Cornell University; Luke Parry, Lancaster University / UFPA. |
Título: |
Explaining the persistence of low income and environmentally degrading land uses in the Brazilian Amazon. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Ecology and Society, v. 22, n. 3, Art. 27, 2017. |
DOI: |
https://doi.org/10.5751/ES-09364-220327 |
Idioma: |
Inglês |
Conteúdo: |
Tropical forests continue to be plagued by the dual sustainability challenges of deforestation and rural poverty. We seek to understand why many of the farmers living in the Brazilian Amazon, home to the world?s largest tropical agricultural-forest frontier, persist in agricultural activities associated with low incomes and high environmental damage. To answer this question, we assess the factors that shape the development and distribution of agricultural activities and farmer well-being in these frontiers. Our study utilizes a uniquely comprehensive social-ecological dataset from two regions in the eastern Brazilian Amazon and employs a novel conceptual framework that highlights the interdependencies between household attributes, agricultural activities, and well-being. We find that livestock production, which yields the lowest per hectare incomes, remains the most prevalent land use in remote areas, but many examples of high income fruit, horticulture, and staple crop production exist on small properties, particularly in peri-urban areas. The transition to more profitable land uses is limited by lagging supply chain infrastructure, social preferences, and the fact that income associated with land use activities is not a primary source of perceived life quality. Instead subjective well-being is more heavily influenced by the nonmonetary attributes of a rural lifestyle (safety, tranquility, community relations, etc.). We conclude that transitions away from low-income land uses in agricultural-forest frontiers of the Brazilian Amazon need not abandon a land-focused vision of development, but will require policies and programs that identify and discriminate households based on a broader set of household assets, cultural attributes, and aspirations than are traditionally applied. At a broader scale, access to distant markets for high value crops must be improved via investments in processing, storage, and marketing infrastructure. MenosTropical forests continue to be plagued by the dual sustainability challenges of deforestation and rural poverty. We seek to understand why many of the farmers living in the Brazilian Amazon, home to the world?s largest tropical agricultural-forest frontier, persist in agricultural activities associated with low incomes and high environmental damage. To answer this question, we assess the factors that shape the development and distribution of agricultural activities and farmer well-being in these frontiers. Our study utilizes a uniquely comprehensive social-ecological dataset from two regions in the eastern Brazilian Amazon and employs a novel conceptual framework that highlights the interdependencies between household attributes, agricultural activities, and well-being. We find that livestock production, which yields the lowest per hectare incomes, remains the most prevalent land use in remote areas, but many examples of high income fruit, horticulture, and staple crop production exist on small properties, particularly in peri-urban areas. The transition to more profitable land uses is limited by lagging supply chain infrastructure, social preferences, and the fact that income associated with land use activities is not a primary source of perceived life quality. Instead subjective well-being is more heavily influenced by the nonmonetary attributes of a rural lifestyle (safety, tranquility, community relations, etc.). We conclude that transitions away from low-income land us... Mostrar Tudo |
Palavras-Chave: |
Capital social; Meios de subsistência sustentáveis; Transições de uso da terra. |
Thesagro: |
Desenvolvimento Rural; Gado; Meio Ambiente. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 02876naa a2200301 a 4500 001 2088106 005 2021-12-22 008 2017 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.5751/ES-09364-220327$2DOI 100 1 $aGARRETT, R. D. 245 $aExplaining the persistence of low income and environmentally degrading land uses in the Brazilian Amazon.$h[electronic resource] 260 $c2017 520 $aTropical forests continue to be plagued by the dual sustainability challenges of deforestation and rural poverty. We seek to understand why many of the farmers living in the Brazilian Amazon, home to the world?s largest tropical agricultural-forest frontier, persist in agricultural activities associated with low incomes and high environmental damage. To answer this question, we assess the factors that shape the development and distribution of agricultural activities and farmer well-being in these frontiers. Our study utilizes a uniquely comprehensive social-ecological dataset from two regions in the eastern Brazilian Amazon and employs a novel conceptual framework that highlights the interdependencies between household attributes, agricultural activities, and well-being. We find that livestock production, which yields the lowest per hectare incomes, remains the most prevalent land use in remote areas, but many examples of high income fruit, horticulture, and staple crop production exist on small properties, particularly in peri-urban areas. The transition to more profitable land uses is limited by lagging supply chain infrastructure, social preferences, and the fact that income associated with land use activities is not a primary source of perceived life quality. Instead subjective well-being is more heavily influenced by the nonmonetary attributes of a rural lifestyle (safety, tranquility, community relations, etc.). We conclude that transitions away from low-income land uses in agricultural-forest frontiers of the Brazilian Amazon need not abandon a land-focused vision of development, but will require policies and programs that identify and discriminate households based on a broader set of household assets, cultural attributes, and aspirations than are traditionally applied. At a broader scale, access to distant markets for high value crops must be improved via investments in processing, storage, and marketing infrastructure. 650 $aDesenvolvimento Rural 650 $aGado 650 $aMeio Ambiente 653 $aCapital social 653 $aMeios de subsistência sustentáveis 653 $aTransições de uso da terra 700 1 $aGARDNER, T. A. 700 1 $aMORELLO, T. F. 700 1 $aMARCHAND, S. 700 1 $aBARLOW, J. 700 1 $aBLAS, D. E. de 700 1 $aFERREIRA, J. N. 700 1 $aLEES, A. C. 700 1 $aPARRY, L. 773 $tEcology and Society$gv. 22, n. 3, Art. 27, 2017.
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Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
10/11/2017 |
Data da última atualização: |
13/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
DOI: |
10.1186/s12866-017-1110-7 |
Idioma: |
Inglês |
Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
Palavras-Chave: |
DNA vaccines. |
Thesagro: |
Corynebacterium Pseudotuberculosis. |
Thesaurus NAL: |
Caseous lymphadenitis; Gene expression; Sheep. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166571/1/Identification-of-new-Corynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500 001 2079448 005 2017-11-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s12866-017-1110-7$2DOI 100 1 $aGALVÃO, C. E. 245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource] 260 $c2017 520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. 650 $aCaseous lymphadenitis 650 $aGene expression 650 $aSheep 650 $aCorynebacterium Pseudotuberculosis 653 $aDNA vaccines 700 1 $aFRAGOSO, S. P. 700 1 $aOLIVEIRA, C. E. de 700 1 $aFORNER, O. 700 1 $aPEREIRA, R. R. B. 700 1 $aSOARES, C. O. 700 1 $aROSINHA, G. M. S. 773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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