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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/08/1999 |
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Data da última atualização: |
11/08/1999 |
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Autoria: |
ELIAS, M.; NAMBISAN, B. SUDHAKRAN, P. R. |
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Afiliação: |
Department of Biochemistry, University of Kerala, Kariavattom, Trivandrum 695 581, India; and Central Tuber Crops Research Institute, Sreekaryam, Trivandrum 695 581, India. |
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Título: |
Characterization of linamarase of latex and its localization in petioles in cassava |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Archives of Biochemistry and Biophysics, v.341, n.2, p.222-228, 1997 |
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Idioma: |
Inglês |
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Conteúdo: |
Yhe distribution of linamarase in latex and its purification, characterization, and immunocytochemical localization in petioles were studied in order to get an insight into the process of cyanogenesis in cassava. Crude latex exudate exhibited low linamarase activity, but on dilution the activity increased about fivefold. Assay using petiole latex collected in isotonic medium showed that the enzyme was distributed in vesicle-like structures. In vitro studies showed that about 50% activity was released from the vesicle into the medium within an hour. Latex linamarase was purified by ammonium sulfate fractionation and DEAE-cellulose chromatography and was characterized with respect to its amino acid composition and kinetic properties. |
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Palavras-Chave: |
Linamarase; Localization; Petiole. |
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Thesagro: |
Latex. |
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Thesaurus Nal: |
cassava; cyanogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01313naa a2200205 a 4500
001 1648233
005 1999-08-11
008 1997 bl --- 0-- u #d
100 1 $aELIAS, M.
245 $aCharacterization of linamarase of latex and its localization in petioles in cassava
260 $c1997
520 $aYhe distribution of linamarase in latex and its purification, characterization, and immunocytochemical localization in petioles were studied in order to get an insight into the process of cyanogenesis in cassava. Crude latex exudate exhibited low linamarase activity, but on dilution the activity increased about fivefold. Assay using petiole latex collected in isotonic medium showed that the enzyme was distributed in vesicle-like structures. In vitro studies showed that about 50% activity was released from the vesicle into the medium within an hour. Latex linamarase was purified by ammonium sulfate fractionation and DEAE-cellulose chromatography and was characterized with respect to its amino acid composition and kinetic properties.
650 $acassava
650 $acyanogenesis
650 $aLatex
653 $aLinamarase
653 $aLocalization
653 $aPetiole
700 1 $aNAMBISAN, B. SUDHAKRAN, P. R.
773 $tArchives of Biochemistry and Biophysics$gv.341, n.2, p.222-228, 1997
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/08/1999 |
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Data da última atualização: |
05/08/1999 |
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Autoria: |
SILVA, W. L. C.; OLIVEIRA, C. A. S.; MAROUELLI, W. A. |
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Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
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Título: |
Subsidios para dimensionamento de sistemas de irrigacao. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE IRRIGACAO E DRENAGEM, 8., 1988, Florianopolis, SC. Anais... Florianopolis: ABID, 1988. |
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Páginas: |
p.535-553. |
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Idioma: |
Português |
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Palavras-Chave: |
Precipitacao; Precipitation. |
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Thesagro: |
Evapotranspiração; Irrigação; Lixiviação; Salinidade; Solo. |
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Thesaurus NAL: |
evapotranspiration; irrigation; leaching; salinity; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00805naa a2200289 a 4500
001 1765038
005 1999-08-05
008 1988 bl uuuu u00u1 u #d
100 1 $aSILVA, W. L. C.
245 $aSubsidios para dimensionamento de sistemas de irrigacao.
260 $c1988
300 $ap.535-553.
650 $aevapotranspiration
650 $airrigation
650 $aleaching
650 $asalinity
650 $asoil
650 $aEvapotranspiração
650 $aIrrigação
650 $aLixiviação
650 $aSalinidade
650 $aSolo
653 $aPrecipitacao
653 $aPrecipitation
700 1 $aOLIVEIRA, C. A. S.
700 1 $aMAROUELLI, W. A.
773 $tIn: CONGRESSO NACIONAL DE IRRIGACAO E DRENAGEM, 8., 1988, Florianopolis, SC. Anais... Florianopolis: ABID, 1988.
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Embrapa Hortaliças (CNPH) |
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