Data/hora: 01/06/2024 12:34:24

Biblioteca(s): | Embrapa Gado de Leite.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 4
Data corrente: | 29/01/2024
Data da última atualização: | 29/01/2024
Autoria: | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.
Afiliação: | CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, CNPGL; LETICIA ZOCCOLARO OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
Título: | Morphology of 16-cell embryo in bovine: Inside cells, compaction, fragmentation and effects of X-sorted semen.
Ano de publicação: | 2024
Fonte/Imprenta: | Anatomia, Histologia, Embryologia, v. 53, e13015, 2024.
DOI: | https://doi.org/10.1111/ahe.13015
Idioma: | Inglês
Conteúdo: | In mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation.
Palavras-Chave: | Compactação; Fertilização in vitro; Fragmentação; Massa celular interna.
Thesagro: | Bovino; Embrião Animal; Reprodução Animal.
Thesaurus Nal: | Embryo (animal).
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161417/1/Morphology-of-16-cell-embryo-in-bovine.pdf
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (26273-1) - UPC - AP - DD -  -  -  - DIS