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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
19/08/2005 |
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Data da última atualização: |
06/11/2006 |
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Autoria: |
PICOLI, E. A. de T.; ALFENAS, A. C.; GONÇALVES, R. C.; DIAS, L. L. C.; NEVES, D. A.; OTONI, W. C.; ROMEIRO, R. da S. |
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Título: |
Detection and antibiotic treatment of Herbaspirillum huttiense isolated from in vitro explants of Eucalyptus sp. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 2, p. 191-198, June 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro assays involving explant cultures aiming to establish a genetic transformation protocol for the Eucalyptus grandis vs. E. smithii hybrid were hampered by the contamination of the explants. One yeast and one bacterium were isolated. The bacterium was identified as Herbaspirillum huttiense, with which further analyses were carried out and its response to different antibiotics were evaluated. Amongst 34 in vitro tested antibiotics, 12 inhibited the bacterial growth. Sulfadiazine, streptomycin, kanamycin and penicillin were selected for determination of the minimal inhibitory concentration. Sulfadiazine, streptomycin and kanamycin presented inhibition halos at 256 and 512 mg l-1, while penicillin did not display bacteriostatic effects in concentrations up to 1024 mg l-1.
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Palavras-Chave: |
Cultura de tecidos vegetais; Endófita; Endophyte; Plant tissue culture. |
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Thesagro: |
Contaminação Bacteriana; Eucalipto. |
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Thesaurus Nal: |
bacterial contamination; Eucalyptus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01672naa a2200289 a 4500
001 1213603
005 2006-11-06
008 2005 bl uuuu u00u1 u #d
100 1 $aPICOLI, E. A. de T.
245 $aDetection and antibiotic treatment of Herbaspirillum huttiense isolated from in vitro explants of Eucalyptus sp.
260 $c2005
520 $aIn vitro assays involving explant cultures aiming to establish a genetic transformation protocol for the Eucalyptus grandis vs. E. smithii hybrid were hampered by the contamination of the explants. One yeast and one bacterium were isolated. The bacterium was identified as Herbaspirillum huttiense, with which further analyses were carried out and its response to different antibiotics were evaluated. Amongst 34 in vitro tested antibiotics, 12 inhibited the bacterial growth. Sulfadiazine, streptomycin, kanamycin and penicillin were selected for determination of the minimal inhibitory concentration. Sulfadiazine, streptomycin and kanamycin presented inhibition halos at 256 and 512 mg l-1, while penicillin did not display bacteriostatic effects in concentrations up to 1024 mg l-1.
650 $abacterial contamination
650 $aEucalyptus
650 $aContaminação Bacteriana
650 $aEucalipto
653 $aCultura de tecidos vegetais
653 $aEndófita
653 $aEndophyte
653 $aPlant tissue culture
700 1 $aALFENAS, A. C.
700 1 $aGONÇALVES, R. C.
700 1 $aDIAS, L. L. C.
700 1 $aNEVES, D. A.
700 1 $aOTONI, W. C.
700 1 $aROMEIRO, R. da S.
773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 5, n. 2, p. 191-198, June 2005.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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