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| 3. |  | BRANDÃO, F. Z.; LEITE, C. R.; FONSECA, J. F. da; FERNANDES, D. A. de M.; BALARO, M. F. A.; SARZEDAS, A. C.; OLIVARES, C. C. S. Dilatação cervical em ovelhas da raça santa inês induzida por misoprostol, ocitocina e estradiol para desenvolvimento de método não cirúrgico para coleta de embriões. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 326.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 4. | | OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z. Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. Small Ruminant Research, Amsterdam, v. 127, p. 44-49, June, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 5. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z. The influence of different methods of frozen-thawed ovine spermatozoa selection on sperm capacitation and viability after incubation. Animal Reproduction, v. 12, n. 3, p. 554, Jul./Sept. 2015. Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20 to 23, 2015. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 199.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. Comparison of different sperm selection techniques in ram frozen-thawed sperm. Acta Scientiae Veterinariae, v. 45, p. 1431, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; SOUZA FABJAN, J. M. G.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z. Influence of mini-Percoll techniques in sperm capacitation and plasma membrane integrity of ram frozen-thawed sperm. Animal Reproduction, v. 13, n. 3, p. 552, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
25/05/2015 |
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Data da última atualização: |
05/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z. |
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Afiliação: |
Carolina Cerqueira Sarmento Olivares, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; André Luís Rios Rodrigues, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; Felipe Zandonadi Brandão, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil. |
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Título: |
Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 127, p. 44-49, June, 2015. |
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DOI: |
doi:10.1016/j.smallrumres.2015.04.009 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Abstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment MenosAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin t... Mostrar Tudo |
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Palavras-Chave: |
Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection. |
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Thesagro: |
Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen. |
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Thesaurus NAL: |
goats; reproduction; spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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024 7 $adoi:10.1016/j.smallrumres.2015.04.009$2DOI
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.$h[electronic resource]
260 $c2015
520 $aAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment
650 $agoats
650 $areproduction
650 $aspermatozoa
650 $aCaprino
650 $aEspermatozóide
650 $aReprodução animal
650 $aSeleção
650 $aSêmen
653 $aCapacitation
653 $aSodium nitroprusside
653 $aSperm preparation
653 $aSperm selection
700 1 $aFONSECA, J. F. da
700 1 $aCAMARGO, L. S. de A.
700 1 $aFABJAN, J. M. G. de S.
700 1 $aRODRIGUES, A. L. R.
700 1 $aBRANDÃO, F. Z.
773 $tSmall Ruminant Research, Amsterdam$gv. 127, p. 44-49, June, 2015.
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