Data/hora: 01/06/2024 11:50:53

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 06/10/2008
Data da última atualização: | 19/02/2009
Autoria: | KIMURA, M.; PEREIRA, M.; FUKUDA, W.; NASCIMENTO, P.; FERNANDES, N.; NUTTI. M.; CARVALHO, J. L.
Afiliação: | Mieko Kimura, UNESP; Marcio Eduardo Canto Pereira, CNPMF; Wania Maria Gonçalves Fukuda, CNPMF; Priscila Nascimento, UNESP; Naiara Fernandes, UNESP; Marilia Nutti, CTAA; José Luiz Carvalho, CTAA.
Título: | Stability of B-carotene during freezer storage of cassava analytical sample.
Ano de publicação: | 2008
Fonte/Imprenta: | In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 36.
Idioma: | Inglês
Notas: | SP01-12.
Conteúdo: | To establish a strategy that would simultaneously attend the breeder's necessity of harvesting a large number of plants and the analytical capacity of the labs, the B-carotene stability during freezer storage of cassava samples was evaluated. Roots of two varieties of cassava growing in an experimental field at EMBRAPA were harvested, peeled and homogenized in a food processor. analytical sample (3-5g) were directly weighed in extraction tubes and frozen. Simultaneoosly, carotenoids of 200g of the same homogenized cassava was extracted with acetone, partitioned to petroleum ether (0,1% BHT) and collected in a volumetric flask. Aliquots were transferred to tubes, dried under nitrogen and kept at -18°C. One half of the samples were kept at Embrapa's laboratory and total  carotenoid content was determined spectrophotometrically every three days. The other half was transported by airplane to UNESP's laboratory and total carotenoid and B-carotene contents were determined by spectrophotometry and HPLC, respectively. Comparison of the results from the two labs showed that the transportation time without refrigeration (12 hrs) did not affect the total carotenoid content of both samples and they were stable during 26 days. Thus, a second experiment was carried out in UNESP's lab using coaxed roots of the same varieties sent by Embrapa. Both, analytical samples and dried extracts, prepared by the same way as in the first experiment and stored at -18°C, were stable over 180 days. These results showed that the analytical samples and dried carotenoid extract can be freezer stored for later analysis. Not having HPLC, the lab can send the dried carotenoid extract to another for B-carotene determination.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)