Data/hora: 08/06/2024 09:21:54

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	15/10/2010	
Data da última atualização: 	30/04/2018	
Autoria: 	COSTA, A. T.; NUNES, W. M. de C.; ZANUTTO, C. A.; MÜLLER, G. W.	
Afiliação: 	Alessandra Tenório Costa, Universidade Estadual de Maringá; William Mário de Carvalho Nunes, Universidade Estadual de Maringá; Carlos Alexandre Zanutto, Universidade Estadual de Maringá; Gerd Walter Müller, Universidade Estadual de Maringá.	
Título: 	Stability of Citrus tristeza virus protective isolates in field conditions.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 693-700, jul. 2010	
Idioma: 	Inglês	
Notas: 	Título em português: Estabilidade de isolados protetores contra Citrus tristeza virus em condições de campo.	
Conteúdo: 	The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products electrophoretic profiles were analyzed using the Jaccard coefficient and the UPGMA method. The majority of the clones had weak to moderate stem pitting symptoms and its CTV isolates showed alterations in the SSCP profiles. However, the stability of the protective complex has been maintained, except for isolates from two analised clones. Low genetic variability was observed within the isolates during the studied years.	
Palavras-Chave: 	Cross-protection; Polimorfismo conformacional da fita simples; Pré?imunização; Preimmunization; Proteção cruzada; Single strand conformational polymorphism; Sintoma de canelura.	
Thesagro: 	Citrus Sinensis.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/105838/1/Stability.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (48300-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS