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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Florestas. |
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Data corrente: |
20/12/2019 |
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Data da última atualização: |
20/12/2019 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
NIEMEYER, J. C.; CARDOSO, E. J. B. N.; NUNES, M. E. T.; ALVES, P. R. L.; MARTINES, A. M.; ANDRÉA, M. M. de; BROWN, G. G.; NIVA, C. C. |
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Afiliação: |
Julia Carina Niemeyer, UFSC; Elke Jurandy Bran Nogueira Cardoso, ESALQ; Maria Edna Tenório Nunes, NEEA/CRHEA/ EESC, USP; Paulo Roger Lopes Alves, Universidade Federal da Fronteira Sul; Alexandre Martin Martines, Martines Consultoria e Projetos; Mara Mercedes de Andréa, Pesquisadora aposentada do do Instituto Biológico; GEORGE GARDNER BROWN, CNPF; CINTIA CARLA NIVA, CPAC. |
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Título: |
Preparo do substrato teste. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: NIVA, C. C.; BROWN, G. G. (Ed.). Ecotoxicologia terrestre: métodos e aplicações dos ensaios com oligoquetas. Brasília, DF: Embrapa, 2019. Cap. 7, p. 143-151. |
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Idioma: |
Português |
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Conteúdo: |
Neste capítulo descrevemos o preparo do solo artificial e natural como substratos para os ensaios ecotoxicológicos com organismos de solo. |
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Palavras-Chave: |
Ensaio ecotoxicológico; Preparo do solo artificial; Preparo do solo natural. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207624/1/2019-CAP-GeorgeB-Ecotoxicologia-Cap7.pdf
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Marc: |
LEADER 00928naa a2200241 a 4500
001 2117406
005 2019-12-20
008 2019 bl uuuu u00u1 u #d
100 1 $aNIEMEYER, J. C.
245 $aPreparo do substrato teste.$h[electronic resource]
260 $c2019
520 $aNeste capítulo descrevemos o preparo do solo artificial e natural como substratos para os ensaios ecotoxicológicos com organismos de solo.
653 $aEnsaio ecotoxicológico
653 $aPreparo do solo artificial
653 $aPreparo do solo natural
700 1 $aCARDOSO, E. J. B. N.
700 1 $aNUNES, M. E. T.
700 1 $aALVES, P. R. L.
700 1 $aMARTINES, A. M.
700 1 $aANDRÉA, M. M. de
700 1 $aBROWN, G. G.
700 1 $aNIVA, C. C.
773 $tIn: NIVA, C. C.; BROWN, G. G. (Ed.). Ecotoxicologia terrestre: métodos e aplicações dos ensaios com oligoquetas. Brasília, DF: Embrapa, 2019. Cap. 7, p. 143-151.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
13/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ANDRADE, J. de S.; MOREIRA, E. M.; SILVA, G. M. da; SCHNEIDER, A.; NUNES, V. R. R.; SILVA, R. R. da; PFEIFER, L. F. M. |
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Afiliação: |
JÉSSICA DE SOUZA ANDRADE, Bionorte, Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Porto Velho, RO; ELIZÂNGELA MÍRIAN MOREIRA, Universidade Federal do Amazonas (UFAM); GEORGE MOREIRA DA SILVA, Universidade Federal de Rondônia (UNIR); AUGUSTO SCHNEIDER, Universidade Federal de Pelotas (UFPel); VANESSA RACHELE RIBEIRO NUNES, CPAF-RO; RENATA REIS DA SILVA, CPAF-RO; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
Uterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 249, 104528, July 2021. |
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ISSN: |
1871-1413 |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104528 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both). MenosThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, trans... Mostrar Tudo |
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Palavras-Chave: |
Citocina; Cytobrush; Inseminação artifical por tempo determinado; PMN cells; Timed artificial insemination (TAI); Uterine health. |
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Thesagro: |
Fertilidade Animal; Gado de Corte; Gado Nelore; Imunidade; Pós-Parto; Reprodução Animal; Sanidade Animal; Útero; Vaca de Corte. |
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Thesaurus NAL: |
Animal fertility; Animal reproduction; Beef cows; Cytokines; Immunity; Nellore; Postpartum period. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03446naa a2200481 a 4500
001 2131781
005 2021-05-13
008 2021 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $ahttps://doi.org/10.1016/j.livsci.2021.104528$2DOI
100 1 $aANDRADE, J. de S.
245 $aUterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome.$h[electronic resource]
260 $c2021
520 $aThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both).
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aBeef cows
650 $aCytokines
650 $aImmunity
650 $aNellore
650 $aPostpartum period
650 $aFertilidade Animal
650 $aGado de Corte
650 $aGado Nelore
650 $aImunidade
650 $aPós-Parto
650 $aReprodução Animal
650 $aSanidade Animal
650 $aÚtero
650 $aVaca de Corte
653 $aCitocina
653 $aCytobrush
653 $aInseminação artifical por tempo determinado
653 $aPMN cells
653 $aTimed artificial insemination (TAI)
653 $aUterine health
700 1 $aMOREIRA, E. M.
700 1 $aSILVA, G. M. da
700 1 $aSCHNEIDER, A.
700 1 $aNUNES, V. R. R.
700 1 $aSILVA, R. R. da
700 1 $aPFEIFER, L. F. M.
773 $tLivestock Science$gv. 249, 104528, July 2021.
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