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| Registros recuperados : 37 | |
| 25. |  | TAVARES, M. A. dos R.; NUNES, M. C. M.; TAVARES, V. E. Q.; FERNANDES, F. F.; SOUSA, L. P. de. Capacidade de uso da terra e abatimento de erosão hídrica em propriedades agrícolas do sul do Rio Grande do Sul. Científica, Jaboticabal, v. 48, n. 4, p. 374-385, 2020.| Biblioteca(s): Embrapa Clima Temperado. |
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| 26. | | CORRÊA-FERREIRA, B. S.; NUNES, M. C.; PERES, W. A. A.; CORSO, I. C.; AZEVEDO, J. de; CHOUCINO, R. Utilização do controle biológico por parasitóides no Manejo Integrado de Pragas da Soja (04.2000.330-02). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: entomologia. Londrina: Embrapa Soja, 2003. p. 31-43. (Embrapa Soja. Documentos, 212).| Biblioteca(s): Embrapa Soja. |
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| 27. | | VIANA, D. S.; CARVALHO, L. M. J. de; PEREIRA, E. J.; CARVALHO, J. L. V. de; NUTTI, M. R.; ROCHA, M. M.; NUNES, M. C. Poliphenols content in raw cowpea (Vigna unguiculata L. Walp.). In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: abstracts. Foz do Iguaçu: IUFoST: ALACCTA, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 28. | | CLEMENTE, Z.; SILVA, G. H. da; NUNES, M. C. S.; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. Exploring the mechanisms of graphene oxide behavioral and morphological changes in zebrafish. Environmental Science and Pollution Research, v. 26, n. 29, p. 30508-30523, 2019.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 29. | | CLEMENTE, Z.; SILVA, G. H. da; MARTINEZ, D. S. T.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; THOMAZ, A. A.; CASTRO, V. L. S. S. de. Exploring the mechanisms of graphene oxide toxicity to zebrafish embryo. Applied Research in Toxicology, v. 2, p. 56, 2017. Suppl. 1. Abstracts of International Symposium on Toxicity Assesment, 18., 2017, Limeira. Ref. ISTA18-705454.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 32. | | CARVALHO, L. M. J. DE.; VIANA, D. S.; PEREIRA, E. J.; CONTE, C.; CARVALHO, J. L. V. de; ROCHA, M. M.; NUNES, M. C.; FREITAS, S. C. de; NUTTI, M. R. Phytic acid in different cowpea (Vigna Unguiculata L. Walp.) cultivars. In: INTERNATIONAL CONFERENCE ON FOOD INOVATION , 3., 2014, Entre Riós. Libro resúmenes. Concordia: Universidad Nacional de Entre Ríos, 2014. p. 359. Ref. A957. 20 al 23 de octubre de 2014.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 33. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Avaliação do conteúdo de carotenoides totais, alfa e beta-caroteno e isômeros cis em abóboras cruas (Cucurbita moschata Duch.). In: REUNIÃO DE BIOFORTIFICAÇÃO NO BRASIL, 4., 2011, Teresina. Palestras e resumos... Rio de Janeiro: Embrapa Agroindústria de Alimentos; Teresina: Embrapa Meio-Norte, 2011. 1 CD-ROM.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 34. | | CARVALHO, M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPCAO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total carotenoids, a and B-carotene and, cis B-carotene isomers in pumpkin (Cucurbita moschata Duch.) cooked with sugar addition. In: INTERNATIONAL ISEKI FOOD CONFERENSCE, 2., 2011, Milan. Abtracts... Milan: ISEKI Food Association, 2011. Resumo em anais.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 35. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPÇÃO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. S. S.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total carotenoids, a and B-carotene and, cis B-carotene isomers in pumpkin (Cucurbita moschata Duch.) cooked with sugar addition. In: INTERNATIONAL ISEKI_FOOD CONFERENCE, 2., 2011, Milan. Bridging training and research for industry and the wider community: book of abstracts. Vienna: ISEKI-Food Association, 2011. Ref. P178.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 36. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPCAO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. R. R.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total catotenoid, a and B-carotene and cis isomers in raw Cucurbita moschata Duch. In: INTERNATIONAL ISEKI FOOD CONFERENSCE, 2., 2011, Milan. Abtracts... Milan: ISEKI Food Association, 2011. Resumo em anais.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 37. | | CARVALHO, L. M. J. de; GODOY, R. L. de O.; PACHECO, S.; ASSUMPÇÃO, S.; GOMES, P. B.; FONTES, R. R.; NUNES, M. C.; GLOBA, M.; RIBEIRO, E. M. G.; RAMOS, S. S. S.; CARVALHO, J. L. V. de; NUTTI, M. R. Assessment of total catotenoid, a and B-carotene and cis isomers in raw Cucurbita moschata Duch. In: INTERNATIONAL ISEKI_FOOD CONFERENCE, 2., 2011, Milan. Bridging training and research for industry and the wider community: book of abstracts. Vienna: ISEKI-Food Association, 2011. Ref. P183.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 37 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/04/2017 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CLEMENTE, Z.; MARTINEZ, D. S. T.; OLIVEIRA, C. R. M.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; CASTRO, V. L. S. S. de. |
|
Afiliação: |
Z. CLEMENTE; D. S. T. MARTINEZ; C. R. M. OLIVEIRA; C. V. MAURER-MORELLI; M. C. S. NUNES; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA. |
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Título: |
Zebrafish exposure to graphene oxide is related to behavior changes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: WORKSHOP IN ENVIRONMENTAL NANOTECHNOLOGY, 2., 2016. Book of Abstracts... Sorocaba: Unesp, 2016. |
|
Páginas: |
p. 27. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Graphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length and yolk sac were measured through a stereomicroscope. The mortality in all groups was below 10% and no malformation was observed. The statistical analysis (Two-way ANOVA followed by Bonferroni test) showed that, independently of HA presence, larvae exposed to GO dispended more time in movement, swam a greater distance, and showed higher swimming velocity than controls. The larvae exposed to GO, with or without HA, were about 3.8% shorter than control. There was no difference among groups related to yolk sac. The effect can be related to GO adsorption to larvae body that can act as tactile stimuli. Our previous study supports the hypothesis that GO can cause impairment in neuromuscular development. Further experiments are being performed to clarify these observations. MenosGraphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length an... Mostrar Tudo |
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Palavras-Chave: |
Nanotoxicology. |
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Thesaurus NAL: |
Ecotoxicology; Embryotoxicity; Nanomaterials. |
|
Categoria do assunto: |
W Química e Física |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161628/1/clemente-zebrafish.pdf
|
|
Marc: |
LEADER 02991nam a2200229 a 4500
001 2068076
005 2017-07-10
008 2016 bl uuuu u00u1 u #d
100 1 $aCLEMENTE, Z.
245 $aZebrafish exposure to graphene oxide is related to behavior changes.$h[electronic resource]
260 $aIn: WORKSHOP IN ENVIRONMENTAL NANOTECHNOLOGY, 2., 2016. Book of Abstracts... Sorocaba: Unesp$c2016
300 $ap. 27.
520 $aGraphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length and yolk sac were measured through a stereomicroscope. The mortality in all groups was below 10% and no malformation was observed. The statistical analysis (Two-way ANOVA followed by Bonferroni test) showed that, independently of HA presence, larvae exposed to GO dispended more time in movement, swam a greater distance, and showed higher swimming velocity than controls. The larvae exposed to GO, with or without HA, were about 3.8% shorter than control. There was no difference among groups related to yolk sac. The effect can be related to GO adsorption to larvae body that can act as tactile stimuli. Our previous study supports the hypothesis that GO can cause impairment in neuromuscular development. Further experiments are being performed to clarify these observations.
650 $aEcotoxicology
650 $aEmbryotoxicity
650 $aNanomaterials
653 $aNanotoxicology
700 1 $aMARTINEZ, D. S. T.
700 1 $aOLIVEIRA, C. R. M.
700 1 $aMAURER-MORELLI, C. V.
700 1 $aNUNES, M. C. S.
700 1 $aCASTRO, V. L. S. S. de
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