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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/07/2015 |
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Data da última atualização: |
03/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARMO, C. D.; SANTOS, D. B.; ALVES, L. B.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de. |
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Afiliação: |
C. D. CARMO, UFRB; D. B. SANTOS, UFRB; L. B. ALVES, UFRB; G. A. F. OLIVEIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporte, 18 April 2015. |
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Páginas: |
14P. |
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Idioma: |
Inglês |
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Conteúdo: |
Cassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers. MenosCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as... Mostrar Tudo |
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Palavras-Chave: |
Marcador molelular. |
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Thesagro: |
Mandioca; Marcador genético. |
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Thesaurus Nal: |
Cassava; Genetic markers. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02362naa a2200241 a 4500
001 2020697
005 2023-03-03
008 2015 bl uuuu u00u1 u #d
100 1 $aCARMO, C. D.
245 $aDevelopment of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava.$h[electronic resource]
260 $c2015
300 $a14P.
520 $aCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers.
650 $aCassava
650 $aGenetic markers
650 $aMandioca
650 $aMarcador genético
653 $aMarcador molelular
700 1 $aSANTOS, D. B.
700 1 $aALVES, L. B.
700 1 $aOLIVEIRA, G. A. F.
700 1 $aOLIVEIRA, E. J. de
773 $tPlant Molecular Biology Reporte, 18 April 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/02/2013 |
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Data da última atualização: |
01/09/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NUNES, J. F.; SIMPLICIO, A. A.; OLIVEIRA, E. R. de; BELLAVER, C.; PORTELLA, J. da S.; MORAES, E. A. de. |
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Afiliação: |
JOSÉ FERREIRA NUNES, CNPC; AURINO ALVES SIMPLÍCIO, CNCP; EDERLON RIBEIRO DE OLIVEIRA, CNPC; CNPC; CNPC; CNPC. |
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Título: |
Comportamento produtivo e reprodutivo de cabras submetidas a duas estações de monta. |
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Ano de publicação: |
1979 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 16., 1979, Curitiba. Anais... Curitiba: SBZ, 1979. v. 1. p. 90. |
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Idioma: |
Português |
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Palavras-Chave: |
Comportamento produtivo; Comportamento reprodutivo; Raça SRD; Sexual behaviour. |
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Thesagro: |
Acasalamento; Cabra; Caprino; Eficiência reprodutiva; Reprodução animal. |
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Thesaurus NAL: |
Animal reproduction; Goats; Reproductive performance. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 00976nam a2200301 a 4500
001 1948285
005 2021-09-01
008 1979 bl uuuu u01u1 u #d
100 1 $aNUNES, J. F.
245 $aComportamento produtivo e reprodutivo de cabras submetidas a duas estações de monta.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 16., 1979, Curitiba. Anais... Curitiba: SBZ, 1979. v. 1. p. 90.$c1979
650 $aAnimal reproduction
650 $aGoats
650 $aReproductive performance
650 $aAcasalamento
650 $aCabra
650 $aCaprino
650 $aEficiência reprodutiva
650 $aReprodução animal
653 $aComportamento produtivo
653 $aComportamento reprodutivo
653 $aRaça SRD
653 $aSexual behaviour
700 1 $aSIMPLICIO, A. A.
700 1 $aOLIVEIRA, E. R. de
700 1 $aBELLAVER, C.
700 1 $aPORTELLA, J. da S.
700 1 $aMORAES, E. A. de
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Embrapa Caprinos e Ovinos (CNPC) |
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