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Registros recuperados : 9 | |
8. | | ARAUJO, R.; MIGLIORANZA, E.; SOUZA, J. R. P. de; ENDO, R. M.; QUINTANA, J. M.; NIENHUIS, J. Concentracao de calcio em frutos de diferentes cultivares de feijao-vagem . Horticultura Brasileira, Brasilia, v.18, p.672-673, jul. 2000. Suplemento. Trabalho apresentado no 40. Congresso Brasileiro de Olericultura, 2000. Biblioteca(s): Embrapa Hortaliças. |
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9. | | BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W. Molecular microbial diversity of an agricultural soil in Wisconsin. Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996. Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 9 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
01/12/1997 |
Data da última atualização: |
01/12/1997 |
Autoria: |
BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W. |
Título: |
Molecular microbial diversity of an agricultural soil in Wisconsin. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996. |
Idioma: |
Inglês |
Conteúdo: |
A culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots. MenosA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were ... Mostrar Tudo |
Thesagro: |
Ecologia; Microrganismo; Solo. |
Thesaurus NAL: |
microbial ecology; soil; Wisconsin. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02390naa a2200277 a 4500 001 1620945 005 1997-12-01 008 1996 bl uuuu u00u1 u #d 100 1 $aBORNEMAN, J. 245 $aMolecular microbial diversity of an agricultural soil in Wisconsin. 260 $c1996 520 $aA culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots. 650 $amicrobial ecology 650 $asoil 650 $aWisconsin 650 $aEcologia 650 $aMicrorganismo 650 $aSolo 700 1 $aSKROCH, P. W. 700 1 $aO'SULLIVAN, K. M. 700 1 $aPALUS, J. A. 700 1 $aRUMJANEK, N. G. 700 1 $aJANSEN, J. L. 700 1 $aNIENHUIS, J. 700 1 $aTRIPLETT, E. W. 773 $tApplied and Environmental Microbiology, Wisconsin$gv. 62, n. 6, p. 1935-1943, 1996.
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